Dynamic profiling of BMSC-dECM reveals accumulation of core matrisome proteins suppresses osteogenic differentiation and bone regeneration DOI Creative Commons
Mei Li,

Weilai Zhu,

Mingyu Hu

и другие.

Journal of Advanced Research, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

The extracellular matrix (ECM) of bone mesenchymal stem cells (BMSCs) plays a critical role in tissue development and regeneration. Rather than being inert, the ECM exhibits dynamic structure that determines cell fate. aim this study is to investigate composition, functional properties, underlying mechanisms BMSC-ECM during osteogenic differentiation. We propose alterations BMSC-ECM, particularly proteins, are essential regulate differentiation Dynamic from BMSCs was collected at different time points culture with or without induction, followed by decellularization. A mouse tibial defect model introduced assess regeneration vivo. Proteomics used analyze protein composition pattern, while comparative transcriptomic analysis further determined impact BMSC-dECM on cellular mRNA profile. Decellularized ECMs (dECMs) late noninduced exhibited distinct properties compared other groups. While early noninduced, (Os)-induced Os-induced dECMs promoted regeneration, dECM dramatically inhibited process. dECMs, rather total content, key factor determining their function. Accumulation core matrisome resulted inhibition its Consistently, gene expression profiles replanted noninduced/Os-induced were similar, leaving separate. Moreover, external negatively regulated intracellular expression. Versican (VCAN) Asporin (ASPN) might be proteins influencing noninduction led solidification inactivation Targeting effectively avoid drawbacks dECM, providing novel strategies for developing highly bioactive cell-derived dECM.

Язык: Английский

Dynamic profiling of BMSC-dECM reveals accumulation of core matrisome proteins suppresses osteogenic differentiation and bone regeneration DOI Creative Commons
Mei Li,

Weilai Zhu,

Mingyu Hu

и другие.

Journal of Advanced Research, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

The extracellular matrix (ECM) of bone mesenchymal stem cells (BMSCs) plays a critical role in tissue development and regeneration. Rather than being inert, the ECM exhibits dynamic structure that determines cell fate. aim this study is to investigate composition, functional properties, underlying mechanisms BMSC-ECM during osteogenic differentiation. We propose alterations BMSC-ECM, particularly proteins, are essential regulate differentiation Dynamic from BMSCs was collected at different time points culture with or without induction, followed by decellularization. A mouse tibial defect model introduced assess regeneration vivo. Proteomics used analyze protein composition pattern, while comparative transcriptomic analysis further determined impact BMSC-dECM on cellular mRNA profile. Decellularized ECMs (dECMs) late noninduced exhibited distinct properties compared other groups. While early noninduced, (Os)-induced Os-induced dECMs promoted regeneration, dECM dramatically inhibited process. dECMs, rather total content, key factor determining their function. Accumulation core matrisome resulted inhibition its Consistently, gene expression profiles replanted noninduced/Os-induced were similar, leaving separate. Moreover, external negatively regulated intracellular expression. Versican (VCAN) Asporin (ASPN) might be proteins influencing noninduction led solidification inactivation Targeting effectively avoid drawbacks dECM, providing novel strategies for developing highly bioactive cell-derived dECM.

Язык: Английский

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