Enhancing lateral flow immunoassay performance for cardiac troponin I detection with pore-size tailored silica nanoparticles and smartphone-based “AdaptiScan” analysis

Frontiers in Bioengineering and Biotechnology, Год журнала: 2025, Номер 13

Опубликована: Март 26, 2025

The accurate and rapid detection of cardiac troponin I (cTnI) at the point care is crucial for timely diagnosis myocardial infarction (MI). This study introduces an advanced lateral flow immunoassay (LFIA) platform cTnI detection. We employed small-sized, large-pore dendritic mesoporous silica nanoparticles (DMSN-2) to encapsulate quantum dots (QDs), achieving enhanced QD loading capacity 1.427 g QD/g silica, compared 0.881 smaller pore counterparts (DMSN-1). nano-LFIA was further integrated with “AdaptiScan”, a smartphone-based system that uses adaptive algorithms automatically extract analyze fluorescence signals from LFIA strips. integration pore-size tailored DMSNs “AdaptiScan” resulted in limit 42.6 ng/L, which meets clinical diagnostic requirements. offers sensitive, cost-effective, portable solution MI, potentially transforming point-of-care testing resource-limited settings.

Язык: Английский

Ultrasensitive nanoenzymes-based lateral flow immunoassay platform for accurate detection of folic acid in human serum

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 163221 - 163221

Опубликована: Май 1, 2025

Язык: Английский