Colloids and Surfaces B Biointerfaces, Год журнала: 2025, Номер unknown, С. 114842 - 114842
Опубликована: Июнь 1, 2025
Язык: Английский
Talanta, Год журнала: 2025, Номер 292, С. 127881 - 127881
Опубликована: Март 11, 2025
microRNAs are small oligonucleotides involved in post-transcriptional gene regulation whose alteration is found several diseases, including cancer, and therefore their detection crucial for diagnosis, prognosis, treatment purposes. Field-Effect Transistor-based biosensors (bioFETs) represent a promising technology the clinical of microRNAs. However, one main challenges associated with this Debye screening, becoming significant at high ionic strengths required effective hybridization. We aimed detecting oncogenic microRNA-155 by using bioFET system as capture element complementary RNA probe (antimiR-155) combined introduction PEG molecules (20 kDa, PEG20), an strength 300 mM. optimized co-immobilization ratio between antimiR-155 PEG20 assessed its impact on interactions oligonucleotides. The kinetics can be well described Langmuir-Freundlich isotherm affinity constant within range typical nucleic acid interactions. significantly enhanced sensitivity miR-155 reaching level less than 200 pM, together excellent discrimination against other clinically relevant Our findings demonstrate that incorporation constitutes strategy to mitigate screening effects facilitates bioFET-based applications physiological strengths.
Язык: Английский
Процитировано
1
ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown
Опубликована: Март 11, 2025
There is an urgent need today for interface management with recognition layers composed of short receptor molecules, excellent specificity and affinity toward a target molecule, wide range sensing applications. The current work demonstrates specific detection G-type nerve agent, which based on nucleophilic substitution reaction between the surface-bound 4-amino-2-((dimethylamino)methyl)phenol (amino-2-DMAMP) receptors diethyl chlorophosphate (DCP) simulant. 2-DMAMP DCP are demonstrated 31P-nuclear magnetic resonance (NMR) electrospray ionization mass spectrometry (ESI-MS/MS). proposed layer utilized through design realization electronic chemosensor using meta-nanochannel field-effect transistor (MNC FET). SiO2 area MNC FET functionalized amino-2-DMAMP amine-based chemistry, response quantified. An demonstrated, coupled limit 1 pg/mL, dynamic 8 orders magnitude, linearity sensitivity. high grade pave way to specific, label-free, quantitative, low-cost, easy-to-operate, field-deployable sensors.
Язык: Английский
Процитировано
0
Advanced Healthcare Materials, Год журнала: 2025, Номер unknown
Опубликована: Апрель 10, 2025
Abstract Biological Field Effect Transistors (Bio‐FETs) are redefining the standard of biosensing by enabling label‐free, real‐time, and extremely sensitive detection biomolecules. At center this innovation is fundamental empowering role advanced materials, such as graphene, molybdenum disulfide, carbon nanotubes, silicon. These when harnessed with downstream biomolecular probes like aptamers, antibodies, enzymes, allow Bio‐FETs to offer unrivaled sensitivity precision. This review an exposition how advancements in materials science have permitted detect biomarkers low concentrations, from femtomolar attomolar levels, ensuring device stability reliability. Specifically, examines incorporation cutting‐edge architectures, flexible / stretchable multiplexed designs, expanding frontiers contributing development more adaptable user‐friendly Bio‐FET platforms. A key focus placed on synergy artificial intelligence (AI), Internet Things (IoT), sustainable approaches fast‐tracking toward transition research into practical healthcare applications. The also explores current challenges material reproducibility, operational durability, cost‐effectiveness. It outlines targeted strategies address these hurdles facilitate scalable manufacturing. By emphasizing transformative played their cementing position Bio‐FETs, positions a cornerstone technology for future solution precision would lead era where herald massive strides biomedical diagnostics subsume.
Язык: Английский
Процитировано
0
Next Nanotechnology, Год журнала: 2025, Номер 8, С. 100170 - 100170
Опубликована: Янв. 1, 2025
Язык: Английский
Процитировано
0
Advanced Sensor Research, Год журнала: 2025, Номер unknown
Опубликована: Май 6, 2025
Abstract The identification of biomarkers is key to the early detection physiological dysfunction. Nanoscale field‐effect transistors (FETs) modified with target‐specific receptors enable direct target sensing, offering enhanced sensitivity due nanoscale channel confinement. In this regard, single‐walled carbon nanotubes (SWCNTs) have emerged as strong candidates for development transistor‐based biosensors. Understanding structural parameters that affect sensing performance in such electrical platforms essential their reliable and controllable use. Here, investigated how different assembly strategies employed construction aptamer‐based SWCNT‐FET biosensors can dramatically signal generation, conductance increasing or decreasing same aptamer‐cortisol recognition event. a cortisol‐binding DNA aptamer exhibiting well‐characterized conformational behavior employed, model receptor explore influence surface functionalization on SWCNT‐based performance. Through combined optical characterization, elucidated conformation governs local electrostatic changes within Debye length, which turn modulates gating devices. This work offers insight into effective design functionalized electrostatically active molecular receptors.
Язык: Английский
Процитировано
0
Colloids and Surfaces B Biointerfaces, Год журнала: 2025, Номер unknown, С. 114842 - 114842
Опубликована: Июнь 1, 2025
Язык: Английский
Процитировано
0