Polypeptide-based
liquid-liquid
phase
separation
(LLPS)
has
received
considerable
attention
as
it
governs
the
formation
of
membraneless
organelles
in
cells.
However,
detailed
mechanistic
understanding
how
one
most
prevalent
cationic
amino
acids
proteins,
arginine,
interacts
with
various
biomolecules
to
induce
and
undergo
morphogenesis
remains
be
resolved.
Herein,
we
report
behavior
transformation
arginine-rich
coacervates
into
vesicular
structures
upon
introducing
polyphosphates.
Transformation
vesicles
was
shown
occur
independent
initial
anionic
counterparts
driven
by
salt-bridge
interactions
between
guanidinium
groups
arginine
residues
phosphates.
We
also
investigate
role
intermolecular
forces
ionic
effects
on
morphological
further
exploit
their
potential
assembly
artificial
tissue-like
constructs.
Overall,
our
findings
underpin
a
unifying
principle
for
vesicle
from
potency
reconstituting
hierarchical
biological
microcompartments.
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(38)
Опубликована: Апрель 29, 2024
Abstract
Adhesives
play
a
crucial
role
in
modern
everyday
applications,
prompting
growing
environmental
consciousness
regarding
their
source,
composition,
utilization,
and
disposal.
Sustainability
concerns
are
frequently
generated
due
to
the
complex
or
energy‐intensive
manufacturing
processes
production
of
conventional
water‐based
adhesives.
Here,
coacervate‐based
snail‐inspired
solvent‐free
adhesive
(SFA)
is
engineered
by
strategically
modulating
electrostatic
interactions
molecular
entanglement
two
polypeptides
under
environmentally
friendly
conditions.
The
SFA
has
record‐breaking
adhesion
strength
28
MPa
on
ceramics,
setting
new
standard
for
bio‐based
demonstrates
robust
performance
across
wide
temperature
ranging
from
−150
300
°C
allows
reusability
without
compromising
properties.
Moreover,
it
can
be
easily
removed
using
non‐toxic
alkaline
solution.
study
presents
promising
opportunity
developing
high‐performance,
reusable
adhesives
made
renewable
materials
suitable
industrial
relics
repair,
even
spacecraft
processing.
Polymers,
Год журнала:
2025,
Номер
17(4), С. 498 - 498
Опубликована: Фев. 14, 2025
Xyloglucan
from
Tamarindus
indica
seeds
(TISs)
is
a
polysaccharide
widely
used
in
the
food,
biomedical,
and
pharmaceutical
sectors.
Nevertheless,
challenge
future
research
for
food
processing
industry
to
provide
adequate
knowledge
regarding
natural
product
extraction,
chemical
modifications,
interactions,
potential
applications
according
sustainability
issues.
The
goal
of
this
work
was
implement
sustainable
method
xyloglucan
extraction
TISs
at
semi-industrial
scale
carry
out
characterization
hydrocolloid,
compare
effect
technique
decorticating
on
composition
physicochemical
properties
xyloglucan.
were
decorticated
using
soaking
(DS)
roasting
(DR)
methods,
and,
then,
extracted
applying
mechanical
separation
process.
Subsequently,
yield,
content,
Fourier
transform
infrared
analysis,
color,
morphology,
molecular
weight
(MW),
viscosity,
texture,
Z
potential,
particle
size,
thermal
evaluated.
demonstrated
first
time.
yield
by
DR
(44.04%)
significantly
higher
(p
<
0.05)
compared
with
DS
(41.42%),
while
efficiency
similar
both
methods
(~97%).
Significant
differences
fat,
ashes,
crude
fiber,
calcium,
total
phenolic
antioxidant
capacity
samples
observed
DR.
promoted
changes
MW
size
samples,
which
reflected
texture
attributes,
These
results
show
that
an
important
issue
be
considered
resultant
tamarind
seeds,
suitable
industries.
Comprehensive Reviews in Food Science and Food Safety,
Год журнала:
2025,
Номер
24(2)
Опубликована: Март 1, 2025
Abstract
Pickering
emulsions
(PEs)
have
attracted
considerable
interest
as
platforms
for
encapsulating
and
controlling
the
release
of
bioactive
compounds.
Recent
studies
emphasize
potential
soybean
protein
nanoparticles
to
improve
PE‐based
carriers,
enhancing
stability
bioavailability
these
compounds
through
unique
self‐assembly
behaviors.
This
review
analyzes
recent
advancements
in
use
nanoparticle‐stabilized
PEs
carriers
Various
fabrication
techniques,
including
physical,
chemical,
biological
methods,
are
explored.
The
effectiveness
nanoparticles,
both
individually
combination
with
polysaccharides
or
polyphenols,
is
evaluated,
highlighting
their
roles
stabilizing
functionality.
Findings
indicate
that
effective
stabilizers
a
wide
range
PE
structures,
oil‐in‐water,
water‐in‐oil,
high
internal
phase
PEs,
emulgels.
Fabrication
properties
particles,
processing
parameters,
formulations
significantly
influence
interfacial
behavior,
structure,
functionality
PEs.
Additionally,
innovative
applications
future
developments
protein–based
discussed,
emphasizing
plant‐based
substitutes
advanced
materials.
Despite
extensive
discussions
on
various
food
forms,
research
into
techno‐functional
flavor
mechanisms
remains
limited.
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Ноя. 21, 2024
Phase-separating
peptides
(PSPs)
self-assembling
into
coacervate
microdroplets
(CMs)
are
a
promising
class
of
intracellular
delivery
vehicles
that
can
release
macromolecular
modalities
deployed
in
wide
range
therapeutic
treatments.
However,
the
molecular
grammar
governing
uptake
and
kinetics
CMs
remains
elusive.
Here,
we
systematically
manipulate
sequence
PSPs
to
unravel
relationships
between
their
structure,
physical
properties
resulting
CMs,
efficacy.
We
show
few
amino
acid
alterations
sufficient
modulate
viscoelastic
towards
either
gel-like
or
liquid-like
state
as
well
binding
interaction
with
cellular
membranes,
collectively
enabling
tune
cargo
release.
also
demonstrate
optimized
display
excellent
transfection
efficiency
hard-to-transfect
cells
such
primary
fibroblasts
immune
cells.
Our
findings
provide
guidelines
precisely
program
material
PSP
achieve
tunable
depending
on
modality,
broad
implications
for
applications
protein,
gene,
cell
therapies.
peptide
(PSP)
have
emerged
vectors,
but
is
authors
establish
designing
optimizing
CM-based
systems.
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(9), С. 4757 - 4764
Опубликована: Фев. 21, 2024
Caseinophosphopeptides
have
shown
great
potential
to
increase
zinc
bioavailability
from
phytate-rich
diets,
but
the
mechanism
of
action
remains
unclear.
Here,
caseinophosphopeptides
a
sodium
caseinate
hydrolysate
dose-dependently
retained
in
solution
against
calcium
phytate
coprecipitation
under
physiologically
relevant
conditions.
The
3
kDa
ultrafiltration
separation
unveiled
no
added
low-molecular-weight
chelates
and
by
caseinophosphopeptides.
Tyndall
effect,
dynamic
light
scattering
measurements,
transmission
electron
microscopy
observation,
diffraction
pattern,
X-ray
spectrum,
energy-dispersive
analysis
demonstrated
caseinophosphopeptides-mediated
formation
single-crystal
zinc/calcium
nanocomplexes
(Zn/CaPA-NCs)
with
size
ζ-potential
10-30
nm
-25
mV,
respectively.
Caseinophosphopeptides-stabilized
Zn/CaPA-NCs
were
found
deliver
bioavailable
nanoparticulate
mouse
jejunal
loop
