Novel Ratiometric Surface-Enhanced Raman Scattering (SERS) Biosensor for Ultrasensitive Quantitative Monitoring of Human Carboxylesterase-1 in Hepatocellular Carcinoma Cells Using Ag–Au Nanoflowers as SERS Substrate
Analytical Chemistry,
Год журнала:
2024,
Номер
96(46), С. 18555 - 18563
Опубликована: Ноя. 5, 2024
In
this
study,
we
developed
ratiometric
surface-enhanced
Raman
scattering
(SERS)
biosensors
using
Ag-Au
alloy
nanoflowers
as
SERS
substrates,
molecules
having
amide
bonds
and
alkyne
groups
(Tag
A)
reporters,
sodium
thiocyanate
an
internal
standard
molecule
B)
for
the
sensitive
detection
of
human
carboxylesterase-1
(hCE1)
in
HepG-2
cells.
The
correlation
between
cell
damage
hCE1
activity
levels
was
investigated.
Both
Tag
A's
group
B's
cyanide
produced
characteristic
signals
Raman-silent
region
(
Язык: Английский
Nanostructured Bubbles‐Enhanced Fluorescence for Ultrasensitive Portable MicroRNA Detection
Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 16, 2024
Abstract
The
unmet
on‐site
clinical
diagnostics
are
increasingly
demanding
portable
bioassays,
but
compromised
by
sensitivity
and
throughput.
Herein,
a
nanostructured
bubbles‐enhanced
fluorescence
(nBEF)
assay
is
reported
interfacing
with
smartphone
imaging
system
to
enable
portable,
ultrasensitive,
specific
miRNA
detection
in
parallel.
In
nBEF,
the
high‐curvature
bubbles
allow
physically
enhancing
optical
propagation,
chemically
fluorescence,
assembling
more
DNA
probes,
undergoing
faster
self‐suspension
efficient
self‐aggregation
enhancement
relative
their
smooth
counterparts.
By
coupling
bubble‐driven
signal
amplification
plug‐and‐play
concatemer,
system,
this
nBEF
achieves
ultrasensitive
fluorescent
of
at
femtomolar
level
one‐base
resolution
single
test,
capable
monitoring
drug‐induced
dynamic
expression
accurate
cancer
diagnosis
(AUC
=
1)
through
profiling
40
plasma
samples
after
RNA
extraction.
This
further
integrates
an
R‐value
analysis
RGB
module
realize
automated,
readout.
Such
high‐performance
provides
low‐cost,
highly
sensitive
tool
diagnosis.
Язык: Английский
Tetrahedral DNA frameworks directed hybridization chain reaction controlled self-assembly
Dongdong He,
Pengyao Wei,
Lin Li
и другие.
Nanoscale Advances,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Tetrahedral
DNA
frameworks
as
restricted
nanotriggers
control
HCR
self-assembled
living
polymerization,
thus
achieving
homogeneous
product
control.
Язык: Английский
Utilizing pillararenes as capping agents to stabilize copper nanoparticles for cost-effective and high-performance SERS application
Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy,
Год журнала:
2025,
Номер
334, С. 125919 - 125919
Опубликована: Фев. 18, 2025
Язык: Английский
Innovative Applications and Perspectives of Surface‐Enhanced Raman Spectroscopy Technology in Biomedicine
Lei Xu,
Yujiao Xie,
Aochi Liu
и другие.
Small,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 28, 2024
Abstract
Surface‐enhanced
Raman
spectroscopy
(SERS)
has
become
a
revolutionary
technique
in
the
biomedical
field,
providing
unparalleled
sensitivity
for
detection
and
characterization
of
biological
samples.
In
this
review,
recent
SERS
innovations
are
comprehensively
discussed,
including
advanced
substrate
materials,
different
strategies,
multimodal
approaches
that
combine
with
other
biotechnologies.
Among
them,
role
accurate
diagnosis
tumors
is
highlighted,
which
promoted
molecular
analysis
real‐time
monitoring
treatment
effects.
addition,
growing
potential
chronic
diseases
such
as
cardiovascular
disease,
diabetes,
neurodegenerative
discussed.
Moreover,
integration
microfluidic
chip
systems
precise
single‐cell
presented.
To
give
forward‐looking
view,
key
challenges
faced
by
technology
also
proposed,
possible
solutions
to
overcome
these
obstacles
provided.
Язык: Английский