The importance of species addition ‘versus’ replacement varies over succession in plant communities after glacier retreat

Nature Plants, Год журнала: 2024, Номер 10(2), С. 256 - 267

Опубликована: Янв. 17, 2024

Язык: Английский

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Environmental DNA methods for biosecurity and invasion biology in terrestrial ecosystems: Progress, pitfalls, and prospects

The Science of The Total Environment, Год журнала: 2024, Номер 926, С. 171810 - 171810

Опубликована: Март 19, 2024

Язык: Английский

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Geographic and Biological Drivers Shape Anthropogenic Extinctions in the Macaronesian Vascular Flora

Global Change Biology, Год журнала: 2025, Номер 31(2)

Опубликована: Фев. 1, 2025

ABSTRACT Whether species extinctions have accelerated during the Anthropocene and extent to which certain are more susceptible extinction due their ecological preferences intrinsic biological traits among most pressing questions in conservation biology. Assessing rates is, however, challenging, as best exemplified by phenomenon of ‘ dark ’: loss that disappear before they even formally described. These issues particularly problematic oceanic islands, where exhibit high endemism unique but also vulnerable extinction. Here, we document plant since Linnaeus' Species Plantarum Macaronesia, a biogeographic region comprised five hyperdiverse archipelagos, identify key drivers behind these extinctions. We compiled 168 records covering 126 taxa, identifying 13 global 155 local events. Significantly higher were observed compared expected background rate. uncovered differentiated patterns along altitudinal gradients, highlighting recent coastal hotspot linked socioeconomic changes Macaronesian archipelagos from 1960s onwards. Key factors influencing include island age, elevation, introduced herbivorous mammals, human population size. Trait‐based analyses across floras Azores Canary Islands revealed endemicity, pollination vertebrates, nitrogen‐fixing capacity, woodiness, zoochory consistently tended increase risk. Our findings emphasize critical role geography traits, alongside anthropogenic impacts, shaping dynamics on islands. Enhancing our knowledge life‐history within is crucial for accurately predicting mitigating future risks, underscoring urgent need comprehensive biodiversity assessments ecosystems.

Язык: Английский

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Investigation of host plant contact with Diaphorina citri by detecting D. citri‐derived environmental DNA

Entomologia Experimentalis et Applicata, Год журнала: 2025, Номер unknown

Опубликована: Фев. 6, 2025

Abstract Citrus greening (Huanglongbing) disease has serious impacts on citrus production. Field monitoring of the Asian psyllid Diaphorina citri (Hemiptera: Psyllidae), a vector disease, is essential to prevent invasion and spread this disease. This study reports new method for determining presence D. traces contact with host plants by collecting leaves from survey area detecting environmental DNA (eDNA) derived . The results show that applied can be determined. As ‐derived eDNA contained in solutions history , we succeeded not only genes conserved mitochondria but also symbiont organisms consisting Wolbachia phage, Candidatus Carsonella sp., Profftella sp. could detected even after 10 min still 6 months contact. technology potential trace their without individual detection expected greatly contribute early warning future.

Язык: Английский

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DNA of the three elements: development of molecular genetic techniques in environmental research

Priroda, Год журнала: 2025, Номер 2(1314), С. 3 - 3

Опубликована: Янв. 1, 2025

Environmental DNA (eDNA) refers to genetic material extracted directly from natural substrates, such as soil, water, or air, rather than a living organism. This innovative approach simplifies the identification of elusive and rare species that are often challenging detect using traditional environmental monitoring methods. For instance, instead deploying camera trap capture nocturnal visitors watering place, eDNA can be analyzed footprints in sand. The origins analysis date back 1980s. Like many transformative ideas, method builds on combination techniques long employed by biologists. Over past four decades, advancements methodology technology have revolutionized field, enabling rapid precise within complex ecological communities.

Язык: Английский

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Soil biome variation of Lupinus nipomensis in wet‐cool vs. dry‐warm microhabitats and greenhouse

American Journal of Botany, Год журнала: 2025, Номер unknown

Опубликована: Март 21, 2025

Environmental DNA (eDNA) can be used to determine the composition of soil biome community, revealing beneficial and antagonistic microbes invertebrates associated with plants. eDNA analyses complement traditional community studies, offering more comprehensive information for conservation practitioners. Studies are also needed examine differences between field greenhouse biomes because greenhouse-grown plants often transplanted in during restoration efforts. We multilocus metabarcoding test how federally state-endangered species, Lupinus nipomensis, differed wet-cool dry-warm microhabitats. At Arroyo Grande, California, 20 experimental plots were sampled, representing a factorial combination vs. that did or not contain L. nipomensis. In simultaneous study, nipomensis was grown drought well-watered conditions compare communities greenhouse. A diversity carbon-cycling microorganisms but nitrogen-fixers overrepresented field, nitrogen-fixing bacteria some treatments. The microbial soils species-rich evenly distributed than communities. plots, microhabitats significantly influenced beta diversity, while without had no significant alpha diversity. Our study shows utility analysis elucidating highlights influence plant on microbe associations.

Язык: Английский

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Influence of homogenization methods on lichen species detection from environmental DNA metabarcoding

Metabarcoding and Metagenomics, Год журнала: 2025, Номер 9

Опубликована: Март 28, 2025

Environmental DNA (eDNA) techniques are increasingly employed in biodiversity monitoring of aquatic and terrestrial animals, plants, fungi, holding great potential to revolutionize assessments. However, sampling basic laboratory protocols still require refinement optimize eDNA metabarcoding performance. Homogenization as a pre-treatment for extraction is known enhance the concentration quality extracted some groups organisms. We previously developed simple efficient method capturing arboreal using stemflow source eDNA; however, its performance with or without homogenization had not yet been compared. In this study, we evaluated two different methods qPCR assays. Metabarcoding analyses revealed that detected fewest species, while nearly identical higher numbers species were samples subjected bead-beating frozen homogenization. Similarly, yielded lowest concentration, yields observed These findings suggest that, considering cost effort, freezing most advantageous.

Язык: Английский

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Biodiversity and Chromosomal Evolution

Опубликована: Янв. 1, 2025

Язык: Английский

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Comparative Analysis of Pasture Composition: DNA Metabarcoding Versus Quadrat‐Based Botanical Surveys in Experimental Grassland Plots

Ecology and Evolution, Год журнала: 2025, Номер 15(4)

Опубликована: Март 27, 2025

ABSTRACT DNA metabarcoding provides a scalable alternative to traditional botanical surveys, which are often time‐consuming and reliant on taxonomic expertise. Here, we compare with quadrat‐based surveys assess plant species composition in experimental grassland plots under four defoliation regimes (continuous grazing, rotational frequent cutting conservation cutting). Botanical identified 16 taxa, while detected 25 including the dominant Holcus lanatus Lolium perenne . Despite detecting more there were some discrepancies identification, sequence data only able resolve taxa at genus level (e.g., Agrostis spp. instead of capillaris ) potential misidentifications Cardaminopsis helleri vs. Cardamine flexuosa ). However, both methods provided comparable results revealed statistically significant differences between treatments, higher diversity cut versus grazed plots. The semi‐quantitative nature limits its capacity accurately reflect abundance, posing challenges for ecological interpretations where precise quantification is required. it broader view biodiversity can complement methods, offering new opportunities efficient monitoring. findings support integration into assessments, particularly when used alongside techniques. Further refinement bioinformatics tools reference databases will enhance their accuracy reliability, enabling effective monitoring sustainable management practices. This study highlights as valuable tool understanding community responses interventions.

Язык: Английский

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Unraveling the potential of environmental DNA for deciphering recent advances in plant–animal interactions: a systematic review

Planta, Год журнала: 2023, Номер 258(6)

Опубликована: Ноя. 13, 2023

Язык: Английский

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