Diagnostic accuracy of bone marrow blood evaluation in Hemophagocytic Lymphohistiocytosis pediatric patients
Annals of Clinical Biochemistry International Journal of Laboratory Medicine,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 17, 2024
Introduction
Haemophagocytic
lymphohistiocytosis
(HLH)
is
a
rare
and
serious
immunological
syndrome
that
involves
strong
activation
of
cytotoxic
T
lymphocytes
macrophages.
HLH
determines
cytokine-mediated
tissue
injury
with
contemporary
multi-organ
failure
high
fatality
rate.
Material
methods
A
retrospective
study
was
performed
considering
the
medical
records
paediatric
patients
who
underwent
bone
marrow
aspirate
for
suspect
HLH.
The
biomarkers
evaluated
were
among
those
included
in
HLH-2004.
Lactate
dehydrogenase
(LD)
also
evaluated.
Haemophagocytosis
blood
smear
slides.
Results
Enrolled
11
group
8
as
controls.
Haemoglobin
fibrinogen
resulted
lower
than
controls,
while
triglycerides,
serum
ferritin
LD
increased.
Blood
triglycerides
discriminated
cases
perfectly,
sensitivity
specificity
100%.
Ferritin
had
100%
83%
(cut
off
≥3,721
µg/L)
73%
(the
cut
≥1,903
U/L).
found
to
have
75%
≤
96
g/L).
Total
haemophagocytes
cell
counts
not
different
between
Only
increased
number
phagocytized
nucleated
red
cells
(NRBC)
be
significantly
patients.
Erythrocytes
phagocytosis
(≥4/1,000
cells)
only
tended
towards
significance.
Conclusions
showed
better
diagnostic
performance
morphological
evaluation.
Among
lineages
engulfed
by
haemophagocytes,
best
obtained
phagocytosed
mature
erythrocytes
immature
erythrocytes.
Язык: Английский
Increased calcification by erythrophagocytosis in aortic valvular interstitial cells
ESC Heart Failure,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 27, 2024
Abstract
Background
Calcific
aortic
valve
disease
(CAVD)
progresses
over
time
to
severe
stenosis
and
eventually
heart
failure.
Recent
evidence
indicates
that
intraleaflet
haemorrhage
(ILH)
strongly
promotes
CAVD
progression.
However,
it
remains
poorly
understood
how
mechanistically
contributes
valvular
calcification.
Method
ILH
was
identified
as
iron
deposition
by
morphological
analysis.
To
elucidate
the
underlying
mechanism,
human
interstitial
cells
(VIC)
were
cultured
in
presence
of
fresh
or
senescent
red
blood
(RBC),
simulating
vivo
conditions.
Result
common
valves
derived
from
patients
with
stenosis.
VIC
undergo
erythrophagocytosis
RBC,
leading
intracellular
accumulation
analogous
observed
following
exposure
extracellular
iron.
The
RBC
significantly
intensified
calcification,
which
mitigated
ferroptosis
inhibition.
Conclusions
Our
results
identify
VIC,
enhanced
calcification
through
ferroptosis.
This
may
be
a
crucial
component
pathophysiological
mechanisms
links
accelerated
Язык: Английский
A potent proresolving mediator 17R-resolvin D2 from human macrophages, monocytes, and saliva
Science Advances,
Год журнала:
2024,
Номер
10(47)
Опубликована: Ноя. 20, 2024
Production
of
specialized
proresolving
mediators
(SPMs)
during
the
resolution
phase
in
acute
inflammatory
response
is
key
to
orchestrating
complete
resolution.
Here,
we
uncovered
a
trihydroxy
resolvin
fresh
human
saliva.
We
identified
and
determined
its
stereochemistry
as
7S,16R,17R-trihydroxy-4Z,8E,10Z,12E,14E,19Z-docosahexaenoic
acid
(17R-RvD2)
using
total
organic
synthesis
matching
physical
properties.
The
17R-RvD2
was
produced
by
activated
M2-like
macrophages,
M1-like
peripheral
blood
monocytes.
displayed
potent
functions
(picomolar
nanomolar).
Topical
application
on
mouse
ear
skin
reduced
neutrophilic
infiltration
(~50%).
increased
M2
markers
CD206
CD163
monocyte–derived
macrophages
enhanced
efferocytosis
senescent
red
cells
(EC
50
~
2.6
×
10
−14
M).
In
addition,
RvD2
receptor
equipotent
epimer
RvD2.
also
significantly
phagocytosis
Escherichia
coli
neutrophils.
Together,
these
results
establish
previously
unknown
17R-RvD2.
Язык: Английский