Tuning Almond Lipase Features by Using Different Immobilization Supports

Catalysts, Год журнала: 2024, Номер 14(2), С. 115 - 115

Опубликована: Янв. 31, 2024

The lipase from Prunus dulcis almonds has been immobilized for the first time. For this purpose, two different supports, an octadecyl methacrylate particulate support, and aminated agarose (monoaminoethyl-N-aminoethyl) have utilized. Both biocatalysts show improved enzyme stability, but great changes in specificity were detected. via ion exchange maintained its activity intact versus p-nitrophenyl butyrate, while on hydrophobic support fully lost substrate, which was confirmed to be due substrate adsorption support. However, biocatalyst much more active triacetin (more than 10-fold), R- or S- methyl mandelate at pH 7. At 9, a strong effect of using phosphate bicarbonate as reaction buffers Using bicarbonate, interfacially presented no R-isomer, it very S-isomer triacetin. buffer during reaction, all compounds recognized substrates. significantly phosphate; fact, inactive both isomers. This paper shows time interaction between effects immobilization protocol used enantiospecificity lipases.

Язык: Английский

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Green synthesis of tetra-substituted 2H-pyrans catalyzed by lipase in aqueous medium

Biocatalysis and Biotransformation, Год журнала: 2025, Номер unknown, С. 1 - 8

Опубликована: Янв. 15, 2025

An efficient and environmental method was employed to synthesize multi-substituted 2H-pyran from methyl coumalate β-keto esters or diketones. Porcine pancreas lipase (PPL) can catalyze this transformation in high yields (74–96%) aqueous medium (H2O: DMSO = 2:1) under mild conditions (60 °C). The reported strategy exhibits functional group compatibility broad substrate scope. In addition, it provides access a variety of tetra-substituted 2H-pyrans which are considered privileged scaffolds with diverse biological activities.

Язык: Английский

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Enzyme-Controlled Bidirectional Enantioselectivity in Asymmetric Decarboxylative Mannich Reaction for Synthesizing β-Sulfonamide Ketones

ACS Catalysis, Год журнала: 2025, Номер unknown, С. 4784 - 4797

Опубликована: Март 6, 2025

Язык: Английский

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Chemoenzymatic synthesis

Communications Chemistry, Год журнала: 2025, Номер 8(1)

Опубликована: Март 13, 2025

Communications Chemistry is pleased to introduce a Collection of research works focused on recent developments within the interdisciplinary field chemoenzymatic synthesis. Here, Guest Editors highlight key themes and look towards future this field.

Язык: Английский

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A Urease-Catalyzed Three-Component Reaction for the Efficient and Sustainable Synthesis of Highly Substituted 4H-Pyrans in Water as the Solvent

Synthesis, Год журнала: 2024, Номер unknown

Опубликована: Сен. 25, 2024

Abstract An efficient urease-catalyzed approach for the synthesis of highly substituted 6-amino-4H-pyran-3-carbonitriles based on formation three bonds in one step is developed. This unprecedented three-component reaction between molecule an aromatic aldehyde and two molecules aroylacetonitrile proceeds by employing commercially available urease from jack bean (Canavalia ensiformis) as catalyst water at 65 °C to deliver desired 4H-pyrans yields up 92%. The transformation proposed occur via a domino Knoevenagel condensation/1,4-addition/O-cyclization/tautomerization sequence, providing practical sustainable substrates. Full unambiguous structural elucidation all products achieved means NMR spectroscopy X-ray crystal structure analysis.

Язык: Английский

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Hemoglobin: An efficient oxidase for the green synthesis of quinazoline derivatives by biocatalytic domino strategy

Molecular Catalysis, Год журнала: 2024, Номер 570, С. 114680 - 114680

Опубликована: Ноя. 21, 2024

Язык: Английский

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