Angewandte Chemie,
Год журнала:
2022,
Номер
134(20)
Опубликована: Фев. 23, 2022
Abstract
Simultaneous
imaging
of
multiple
microRNAs
(miRNAs)
in
individual
living
cells
is
challenging
due
to
the
lack
spectrally
distinct
encoded
fluorophores
and
non‐cytotoxic
methods.
We
describe
a
multiplexed
error‐robust
combinatorial
fluorescent
label‐encoding
method,
termed
error‐corrected
labels
(FluoELs),
enabling
miRNA
with
error‐correcting
capability.
The
FluoELs
comprise
proportional
dual
for
encoding
constant
quantitative
single
fluorophore
quantification.
Both
are
embedded
260
nm
core–shell
silica
nanoparticles
modified
molecular
beacon
detection
probes.
low
cytotoxic
could
accurately
quantify
spatially
resolve
nine
breast‐cancer‐related
miRNAs
evaluate
their
coordination.
enabled
single‐cell
analysis
platform
expression
profiles
mechanisms
underlying
miRNA‐associated
diseases.
Angewandte Chemie,
Год журнала:
2022,
Номер
134(45)
Опубликована: Июль 1, 2022
Abstract
The
wide
extracellular‐intracellular
distribution
of
microRNA
requires
the
on‐site,
robust
and
efficient
activation
catalytic
DNA
circuits
inside
live
cells.
Herein,
we
develop
an
non‐enzymatic
circuitry
strategy
to
realize
orthogonally
controlled
(CCD)
circuit
for
achieving
high‐fidelity
in
vivo
imaging
through
multiply
guaranteed
molecular
recognition
progressively
accelerated
signal
amplification.
For
predictable
on‐site
useful
efficiency,
dominating
fuel
strand
was
initially
split
into
inactive
subunits
that
were
grafted
auxiliary
circuit.
There,
in‐cell‐specific
mRNA
triggered
orthogonal
amplification
active
strands
sensitive
target
detection
chief
entropy‐driven
We
believe
method
can
contribute
clinical
diagnosis
prognosis.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(42), С. 16971 - 16977
Опубликована: Окт. 11, 2024
The
development
of
highly
sensitive
and
precise
imaging
techniques
capable
visualizing
crucial
molecules
at
the
subcellular
level
is
essential
for
elucidating
mitochondrial
functions
uncovering
novel
mechanisms
in
biological
processes.
However,
traditional
molecular
strategies
are
still
limited
by
off-mitochondria
signal
leakage
because
"always-active"
sensing
mode.
To
address
this
limitation,
we
have
developed
a
light-triggered
activation
sequence
activated
plasmonic
DNAzyme
walker
(PDW)
accurate
combination
an
organelle
localized
strategy,
upconversion
nanotechnology,
plasmon
enhanced
fluorescence
(PEF)
technique.
Exploiting
advantage
light
enables
control
over
when
where
to
activate
probe's
function,
effectively
reducing
as
validated
dynamic
monitoring
changes
signals
during
entry
process.
Furthermore,
leveraging
PEF
capability
triangular
gold
nanoprisms
(Au
NPRs),
intensity
can
be
approximately
11.9
times,
ensuring
imaging.
Angewandte Chemie,
Год журнала:
2022,
Номер
134(20)
Опубликована: Фев. 23, 2022
Abstract
Simultaneous
imaging
of
multiple
microRNAs
(miRNAs)
in
individual
living
cells
is
challenging
due
to
the
lack
spectrally
distinct
encoded
fluorophores
and
non‐cytotoxic
methods.
We
describe
a
multiplexed
error‐robust
combinatorial
fluorescent
label‐encoding
method,
termed
error‐corrected
labels
(FluoELs),
enabling
miRNA
with
error‐correcting
capability.
The
FluoELs
comprise
proportional
dual
for
encoding
constant
quantitative
single
fluorophore
quantification.
Both
are
embedded
260
nm
core–shell
silica
nanoparticles
modified
molecular
beacon
detection
probes.
low
cytotoxic
could
accurately
quantify
spatially
resolve
nine
breast‐cancer‐related
miRNAs
evaluate
their
coordination.
enabled
single‐cell
analysis
platform
expression
profiles
mechanisms
underlying
miRNA‐associated
diseases.
