High‐Throughput Colorimetric Detection and Quantification of Indoles and Pyrroloindoles for Enzymatic Activity Determination DOI Creative Commons
Diana A. Amariei, Mona Haase, Moritz K. T. Klischan

и другие.

ChemCatChem, Год журнала: 2024, Номер 16(11)

Опубликована: Фев. 20, 2024

Abstract Indoles and pyrroloindoles are structural motifs present in many biologically active natural products. Multiple classes of enzymes catalyze the transformation indoles into via group transfer followed by intramolecular cyclization, such as peroxydases, methyltransferases, prenyltransferases. Due to selective introduction a stereogenic center, these receive increasing attention catalytic tools for production pharmacologically relevant compounds. Two new colorimetric assays described this work, which allow quantification enzymatic reactions from perspective substrate product. For substrates, indole assay is based on modified version Ehrlich test, with use light driving force color formation. The pyrroloindole uses cerium sulfate reagent complementary both were successfully utilized activity determination C3‐indole methyltransferase. They can facilitate high‐throughput screening mutant libraries, offering support engineering enzymes, but also be used stand‐alone methods detection

Язык: Английский

High‐Throughput Colorimetric Detection and Quantification of Indoles and Pyrroloindoles for Enzymatic Activity Determination DOI Creative Commons
Diana A. Amariei, Mona Haase, Moritz K. T. Klischan

и другие.

ChemCatChem, Год журнала: 2024, Номер 16(11)

Опубликована: Фев. 20, 2024

Abstract Indoles and pyrroloindoles are structural motifs present in many biologically active natural products. Multiple classes of enzymes catalyze the transformation indoles into via group transfer followed by intramolecular cyclization, such as peroxydases, methyltransferases, prenyltransferases. Due to selective introduction a stereogenic center, these receive increasing attention catalytic tools for production pharmacologically relevant compounds. Two new colorimetric assays described this work, which allow quantification enzymatic reactions from perspective substrate product. For substrates, indole assay is based on modified version Ehrlich test, with use light driving force color formation. The pyrroloindole uses cerium sulfate reagent complementary both were successfully utilized activity determination C3‐indole methyltransferase. They can facilitate high‐throughput screening mutant libraries, offering support engineering enzymes, but also be used stand‐alone methods detection

Язык: Английский

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