Active droplets through enzyme-free, dynamic phosphorylation
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Май 17, 2024
Abstract
Life
continuously
transduces
energy
to
perform
critical
functions
using
stored
in
reactive
molecules
like
ATP
or
NADH.
dynamically
phosphorylates
active
sites
on
proteins
and
thereby
regulates
their
function.
Inspired
by
such
machinery,
regulating
supramolecular
has
gained
traction.
Enzyme-free,
synthetic
systems
that
use
dynamic
phosphorylation
regulate
processes
have
not
yet
been
reported,
our
knowledge.
Here,
we
show
an
enzyme-free
reaction
cycle
consumes
the
phosphorylating
agent
monoamidophosphate
transiently
histidine
histidine-containing
peptides.
The
phosphorylated
species
are
labile
deactivate
through
hydrolysis.
exhibits
versatility
tunability,
allowing
for
of
multiple
precursors
with
a
tunable
half-life.
Notably,
resulting
products
can
peptide’s
phase
separation,
leading
droplets
require
continuous
conversion
fuel
sustain.
will
be
valuable
as
model
biological
but
also
offer
insights
into
protocell
formation.
Язык: Английский
Non‐Equilibrium Dissipative Assembly with Switchable Biological Functions
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
63(48)
Опубликована: Авг. 22, 2024
Abstract
Natural
dissipative
assembly
(DSA)
often
exhibit
energy‐driven
shifts
in
natural
functions.
However,
creating
man‐made
DSA
that
can
mimic
such
biological
activities
transformation
remains
relatively
rare.
Herein,
we
introduce
a
cytomembrane‐like
system
based
on
chiral
supramolecules.
This
employs
benzoyl
cysteine
an
out
of
equilibrium
manner,
enabling
the
biofunctions
while
minimizing
material
use.
Specifically,
aroyl‐cystine
derivatives
primarily
assemble
into
stable
M‐helix
nanofibers
under
conditions.
These
enhance
fibroblast
adhesion
and
proliferation
through
stereospecific
interactions
with
cellular
membranes.
Upon
addition
chemical
fuels,
these
functional
temporarily
transform
non‐equilibrium
nanospheres,
facilitating
efficient
drug
delivery.
Subsequently,
nanospheres
revert
to
their
original
nanofiber
state,
effectively
recycling
drug.
The
programmable
function‐shifting
ability
this
establishes
it
as
novel,
fuel‐driven
delivery
vehicle.
And
bioactive
not
only
addresses
gap
synthetic
DSAs
within
applications
but
also
sets
stage
for
innovative
designs
′living′
materials.
Язык: Английский
Non‐Equilibrium Dissipative Assembly with Switchable Biological Functions
Angewandte Chemie,
Год журнала:
2024,
Номер
136(48)
Опубликована: Авг. 22, 2024
Abstract
Natural
dissipative
assembly
(DSA)
often
exhibit
energy‐driven
shifts
in
natural
functions.
However,
creating
man‐made
DSA
that
can
mimic
such
biological
activities
transformation
remains
relatively
rare.
Herein,
we
introduce
a
cytomembrane‐like
system
based
on
chiral
supramolecules.
This
employs
benzoyl
cysteine
an
out
of
equilibrium
manner,
enabling
the
biofunctions
while
minimizing
material
use.
Specifically,
aroyl‐cystine
derivatives
primarily
assemble
into
stable
M‐helix
nanofibers
under
conditions.
These
enhance
fibroblast
adhesion
and
proliferation
through
stereospecific
interactions
with
cellular
membranes.
Upon
addition
chemical
fuels,
these
functional
temporarily
transform
non‐equilibrium
nanospheres,
facilitating
efficient
drug
delivery.
Subsequently,
nanospheres
revert
to
their
original
nanofiber
state,
effectively
recycling
drug.
The
programmable
function‐shifting
ability
this
establishes
it
as
novel,
fuel‐driven
delivery
vehicle.
And
bioactive
not
only
addresses
gap
synthetic
DSAs
within
applications
but
also
sets
stage
for
innovative
designs
′living′
materials.
Язык: Английский