Spatially Selective Imaging of Cell–Matrix and Cell–Cell Junctions by Electrochemiluminescence

Angewandte Chemie International Edition, Год журнала: 2021, Номер 60(21), С. 11769 - 11773

Опубликована: Март 12, 2021

Abstract Cell junctions are protein structures located at specific cell membrane domains that determine key processes in multicellular development. Here we report spatially selective imaging of by electrochemiluminescence (ECL) microscopy. By regulating the concentrations luminophore and/or co‐reactant, thickness ECL layer can be controlled to match with spatial location different junctions. At a low concentration luminophore, generation is confined electrode surface, thus revealing only cell–matrix adhesions bottom cells. While high remarkably extended decreasing co‐reactant concentration, allowing sequential and cell–cell near apical surface cells, respectively. This strategy not provides new insights into mechanisms but also promises wide applications microscopy bioimaging.

Язык: Английский

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Electrogenerated chemiluminescence detection of single entities

Chemical Science, Год журнала: 2021, Номер 12(16), С. 5720 - 5736

Опубликована: Янв. 1, 2021

Electrogenerated chemiluminescence, also known as electrochemiluminescence (ECL), is an electrochemically induced production of light by excited luminophores generated during redox reactions. It can be used to sense the charge transfer and related processes at electrodes via a simple visual readout; hence, ECL outstanding tool in analytical sensing. The traditional approach measures averaged electrochemical quantities large ensemble individual entities, including molecules, microstructures ions. However, real system usually heterogeneous, study single entities holds great potential elucidating new truths nature which are out assays or hidden complex systems. We would like review development intensity imaging based entity detection place emphasis on small micro/nanoparticles cells. current challenges for perspectives discussed.

Язык: Английский

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The New Era of High-Throughput Nanoelectrochemistry

Analytical Chemistry, Год журнала: 2023, Номер 95(1), С. 319 - 356

Опубликована: Янв. 10, 2023

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTThe New Era of High-Throughput NanoelectrochemistryXiangdong XuXiangdong XuDepartment Chemistry, University Warwick, Coventry CV4 7AL, U.K.More by Xiangdong XuView Biographyhttps://orcid.org/0000-0002-1120-4661, Dimitrios ValavanisDimitrios ValavanisDepartment ValavanisView Biographyhttps://orcid.org/0000-0002-8777-664X, Paolo CiocciPaolo CiocciUniversité Paris Cité, ITODYS, CNRS, F-75013 Paris, FranceMore CiocciView Biography, Samuel ConfederatSamuel ConfederatSchool Electronic and Electrical Engineering Pollard Institute, Leeds, Leeds LS2 9JT, U.K.Bragg Centre for Materials Research, ConfederatView Fabio MarcuccioFabio MarcuccioSchool U.K.Faculty Medicine, Imperial College London, London SW7 2AZ, United KingdomMore MarcuccioView Biographyhttps://orcid.org/0000-0003-4816-2896, Jean-François LemineurJean-François LemineurUniversité LemineurView Actis*Paolo ActisSchool U.K.*[email protected]More ActisView Biographyhttps://orcid.org/0000-0002-7146-1854, Frédéric Kanoufi*Frédéric KanoufiUniversité France*[email KanoufiView Biographyhttps://orcid.org/0000-0002-9784-2380, Patrick R. Unwin*Patrick UnwinDepartment UnwinView Biographyhttps://orcid.org/0000-0003-3106-2178Cite this: Anal. Chem. 2023, 95, 1, 319–356Publication Date (Web):January 10, 2023Publication History Received15 November 2022Published online10 January 2023Published inissue 10 2023https://doi.org/10.1021/acs.analchem.2c05105Copyright © 2023 The Authors. Published American Chemical SocietyRIGHTS & PERMISSIONSACS AuthorChoiceCC: Creative CommonsBY: Credit must be given to the creatorArticle Views1582Altmetric-Citations1LEARN ABOUT THESE METRICSArticle Views are COUNTER-compliant sum full text article downloads since 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InReddit (5 MB) Get e-AlertsSUBJECTS:Ions,Nanoparticles,Nanopores,Probes,Surface charge e-Alerts

Язык: Английский

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Single Cell Imaging of Electrochemiluminescence‐Driven Photodynamic Therapy

Angewandte Chemie International Edition, Год журнала: 2022, Номер 61(16)

Опубликована: Фев. 15, 2022

Abstract We report a photodynamic therapy driven by electrochemiluminescence (ECL). The luminescence generated Ru(bpy) 3 2+ and co‐reactant tripropylamine (TPA) pair acts as both optical readout for ECL imaging, light source the excitation of photosensitizer to produce reactive oxygen species (ROS) in (PDT) system. ECL‐driven PDT (ECL‐PDT) relies on effective energy transfer from emission chlorin e6 (Ce6), which sensitizes surrounding O 2 into ROS. dynamic process gradual morphological changes, variation cell‐matrix adhesions, well increase cell membrane permeability ECL‐PDT were monitored under microscopy (ECLM) with good spatiotemporal resolution. Combining real‐time imaging ECL‐PDT, this new strategy provides not only insights cellular processes, but also promising potential clinical applications.

Язык: Английский

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Ultrasensitive Imaging of Cells and Sub‐Cellular Entities by Electrochemiluminescence

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(16)

Опубликована: Фев. 22, 2023

Here we report on a label-free electrochemiluminescence (ECL) microscopy using exceptionally low concentrations of the [Ru(bpy)3 ]2+ luminophore. This work addresses central point minimal concentration ECL luminophore required to image single entities. We demonstrate possibility record images cells and mitochondria at down nM pM. is 7 orders magnitude lower than classically-used corresponds few hundreds luminophores diffusing around biological Yet, it produces remarkably sharp negative optical contrast images, as demonstrated by structural similarity index metric analyses supported predictions covering time. Finally, show that reported approach simple, fast, highly sensitive method, which opens new avenues for ultrasensitive imaging reactivity molecule level.

Язык: Английский

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Photochemical Strategies toward Precision Targeting against Multidrug-Resistant Bacterial Infections

ACS Nano, Год журнала: 2024, Номер 18(22), С. 14085 - 14122

Опубликована: Май 22, 2024

Infectious diseases pose a serious threat and substantial economic burden on global human public health security, especially with the frequent emergence of multidrug-resistant (MDR) bacteria in clinical settings. In response to this urgent need, various photobased anti-infectious therapies have been reported lately. This Review explores discusses several photochemical targeted antibacterial therapeutic strategies for addressing bacterial infections regardless their antibiotic susceptibility. contrast conventional therapies, these approaches facilitate precise targeting pathogenic and/or infectious microenvironments, effectively minimizing toxicity mammalian cells surrounding healthy tissues. The highlighted include photodynamic therapy, photocatalytic photothermal endogenous pigments-based photobleaching polyphenols-based photo-oxidation therapy. comprehensive exploration aims offer updated information development effective, convenient, safe, alternative counter growing MDR future.

Язык: Английский

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Electrochemiluminescence Microscopy

Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(29)

Опубликована: Май 14, 2024

Electrochemiluminescence (ECL) is rapidly evolving from an analytical method into optical microscopy. The orthogonality of the electrochemical trigger and readout distinguishes it classic microscopy techniques, owing to its near-zero background, remarkable sensitivity, absence photobleaching phototoxicity. In this minireview, we summarize recent advances in ECL imaging technology, emphasizing original configurations which enable biological entities improvement properties by increasing complexity multiplexing bioassays. Additionally, mapping (electro)chemical reactivity space provides valuable information on nanomaterials facilitates deciphering mechanisms for improving their performances diagnostics (electro)catalysis. Finally, highlight achievements at ultimate limits single molecules, photons or chemical reactions, current challenges translate other fields such as material science, catalysis biology.

Язык: Английский

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Shadow Electrochemiluminescence Microscopy of Single Mitochondria

Angewandte Chemie International Edition, Год журнала: 2021, Номер 60(34), С. 18742 - 18749

Опубликована: Июнь 11, 2021

Mitochondria are the subcellular bioenergetic organelles. The analysis of their morphology and topology is essential to provide useful information on activity metabolism. Herein, we report a label-free shadow electrochemiluminescence (ECL) microscopy based spatial confinement ECL-emitting reactive layer image single living mitochondria deposited electrode surface. ECL mechanism freely-diffusing [Ru(bpy)3 ]2+ dye with sacrificial tri-n-propylamine coreactant restrains light-emitting region micrometric thickness allowing visualize individual remarkable sharp negative optical contrast. imaging approach named "shadow ECL" (SECL) reflects imprint local diffusional hindrance reagents by each mitochondrion. statistical colocalization spots functional revealed classical fluorescent biomarkers, MitoTracker Deep Red endogenous intramitochondrial NADH, validates reported methodology. versatility extreme sensitivity further demonstrated visualizing mitochondria, which remain hardly detectable usual biomarkers. Finally, alleviating problems photobleaching phototoxicity associated conventional methods, SECL should find promising applications in structures.

Язык: Английский

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Recent advances in electrochemiluminescence imaging analysis

eScience, Год журнала: 2022, Номер 2(6), С. 591 - 605

Опубликована: Ноя. 1, 2022

Electrochemiluminescence (ECL) has been widely applied in imaging owing to features that distinguish it from other microscopic techniques and electrochemical methods, including its high signal-to-noise ratio, remarkable sensitivity, wide linear range, spatiotemporal resolution, near-zero background light. Imaging technology based on ECL used the fields of immunosensing, pathological cell detection, drug analysis. Additionally, simple operation ability detect dynamic processes catalytic sites strengthen potential for research material surfaces interfaces, vivo biological analysis, visualization. At same time, emergence a variety nanomaterials new analysis equipment further promoted development high-resolution technology. This paper introduces mechanisms main systems. It then describes various forms reviews progress single-particle imaging, fingerprint structure single-cell imaging. Finally, authors offer their views about prospects

Язык: Английский

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Emerging Optical Microscopy Techniques for Electrochemistry

Annual Review of Analytical Chemistry, Год журнала: 2022, Номер 15(1), С. 57 - 82

Опубликована: Фев. 26, 2022

An optical microscope is probably the most intuitive, simple and commonly used instrument to observe objects discuss behaviors through images. Although idea of imaging electrochemical processes operando by microscopy was initiated 40 years ago, it not until significant progress made in last two decades advanced or plasmonics that could become a mainstream electroanalytical strategy. This review illustrates potential different microscopies visualize quantify local with unprecedented temporal spatial resolution (below diffraction limit), up single object level subnanoparticle molecule sensitivity. Developed optically electrochemically active model systems, now shifting materials configurations focused on real-world applications.

Язык: Английский

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