Encapsulating
living
cells
within
nanoshells
offers
an
important
approach
to
enhance
their
stability
against
environmental
stressors
and
broaden
application
scope.
However,
this
often
leads
impaired
mass
transfer
at
the
cell
biointerface.
Strengthening
protective
shell
with
well-defined,
ordered
transport
channels
is
crucial
regulating
molecular
maintaining
viability
biofunctionality.
Herein,
we
report
construction
of
covalent
organic
framework
(COF)
mesoporous
shells
for
single-cell
nanoencapsulation,
providing
selective
permeability
comprehensive
protection
microbial
cells.
The
COF
ensure
nutrient
uptake
while
blocking
large
harmful
molecules
UV-C
radiation,
thereby
preserving
metabolic
activity.
Integration
such
crystalline
porous
genetically
modified
factories
production
further
investigated,
revealing
no
adverse
effects,
as
demonstrated
by
riboflavin
production.
Moreover,
effectively
shields
cells,
ensuring
efficient
bioproduction
even
after
being
treated
under
harsh
conditions.
This
versatile
encapsulation
applicable
different
types,
a
robust
platform
surface
engineering.
Materials Chemistry Frontiers,
Год журнала:
2023,
Номер
7(16), С. 3298 - 3331
Опубликована: Янв. 1, 2023
This
review
provides
an
overview
of
the
recent
advances
in
heterogeneous
catalysis
using
COFs,
primarily
from
2020
to
2023,
covering
fundamentals,
advantages,
current
challenges,
and
future
perspectives.
Journal of the American Chemical Society,
Год журнала:
2023,
Номер
145(24), С. 13469 - 13475
Опубликована: Июнь 7, 2023
Immobilization
of
fragile
enzymes
in
crystalline
porous
materials
offers
new
opportunities
to
expand
the
applications
biocatalysts.
However,
limited
by
pore
size
and/or
harsh
synthesis
conditions
hosts,
often
suffer
from
dimension
limitation
or
denaturation
during
immobilization
process.
Taking
advantage
dynamic
covalent
chemistry
feature
organic
frameworks
(COFs),
herein,
we
report
a
preprotection
strategy
encapsulate
COFs
self-repairing
and
crystallization
Enzymes
were
first
loaded
low-crystalline
polymer
networks
with
mesopores
formed
at
initial
growth
stage,
which
could
offer
effective
protection
for
reaction
conditions,
subsequently
encapsulation
proceeded
disordered
into
framework.
Impressively,
biological
activity
can
be
well-maintained
after
encapsulation,
obtained
enzyme@COFs
also
show
superior
stability.
Furthermore,
circumvents
enzymes,
its
versatility
was
verified
different
sizes
surface
charges,
as
well
two-enzyme
cascade
system.
This
study
universal
design
idea
robust
supports
holds
promise
developing
high-performance
immobilized
Angewandte Chemie International Edition,
Год журнала:
2023,
Номер
62(23)
Опубликована: Апрель 6, 2023
Abstract
Selecting
a
suitable
support
material
for
enzyme
immobilization
with
excellent
biocatalytic
activity
and
stability
is
critical
aspect
in
the
development
of
functional
biosystems.
The
highly
stable
metal‐free
properties
covalent‐organic
frameworks
(COFs)
make
them
ideal
supports
immobilization.
Herein,
we
constructed
three
kinds
COFs
via
biofriendly
one‐pot
synthetic
strategy
at
room
temperature
aqueous
solution.
Among
developed
(COF‐LZU1,
RT‐COF‐1
ACOF‐1),
horseradish
peroxidase
(HRP)‐incorporated
COF‐LZU1
found
to
retain
highest
activity.
Structural
analysis
reveals
that
weakest
interaction
between
hydrated
COF‐LZU1,
an
easiest
accessibility
by
substrate,
as
well
optimal
conformation
together
promote
bioactivity
HRP‐COF‐LZU1.
Furthermore,
revealed
be
versatile
nanoplatform
encapsulating
multiple
enzymes.
also
offers
superior
protection
immobilized
enzymes
under
harsh
conditions
during
recycling.
comprehensive
understanding
interfacial
interactions
COF
host
guest,
substrate
diffusion,
alteration
within
matrices
represents
opportunity
design
biocatalysts
opens
broad
range
applications
these
nanosystems.
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Июнь 20, 2023
Abstract
Rational
integration
of
native
enzymes
and
nanoscaffold
is
an
efficient
means
to
access
robust
biocatalyst,
yet
remains
on-going
challenges
due
the
trade-off
between
fragile
harsh
assembling
conditions.
Here,
we
report
a
supramolecular
strategy
enabling
in
situ
fusion
into
porous
crystal.
A
c2-symmetric
pyrene
tecton
with
four
formic
acid
arms
utilized
as
building
block
engineer
this
hybrid
biocatalyst.
The
decorated
afford
tectons
high
dispersibility
minute
amount
organic
solvent,
permit
hydrogen-bonded
linkage
discrete
extended
network
around
enzyme
almost
solvent-free
aqueous
solution.
This
biocatalyst
covered
by
long-range
ordered
pore
channels,
which
can
serve
gating
sieve
catalytic
substrate
thus
enhance
biocatalytic
selectivity.
Given
structural
integration,
biocatalyst-based
electrochemical
immunosensor
developed,
pg/mL
detection
cancer
biomarker.
Chemistry of Materials,
Год журнала:
2023,
Номер
35(20), С. 8353 - 8370
Опубликована: Окт. 12, 2023
Covalent
organic
frameworks
(COFs)
make
up
an
emerging
class
of
crystalline
porous
materials
mainly
composed
light
elements
in
the
form
dynamic
covalent
bonds.
Owing
to
their
two-
or
three-dimensional
network
structures
and
ideal
properties,
including
low
density,
large
specific
surface
area,
high
chemical
stability,
good
biocompatibility,
COFs
have
shown
a
wide
range
applications
optoelectronic
devices,
energy
conversion
storage,
adsorption
separation,
sensing,
catalysis,
biomedicine.
This
review
provides
overview
recent
advances
functional
COF-based
nanoplatforms
for
biological
diagnosis
treatment,
such
as
enzyme
protein
drug
delivery,
photodynamic/photothermal
therapy,
synergistic
treatment.
Challenges
future
directions
developing
biomedicine
related
are
also
discussed.
We
envisage
that
this
will
inspire
scientists,
chemists,
biologists,
clinical
doctors
working
fields
work
closely
move
field
toward
trials
human
healthcare.
