Applied Microbiology and Biotechnology,
Год журнала:
2024,
Номер
108(1)
Опубликована: Июль 2, 2024
Abstract
Polyethylene
terephthalate
(PET)
is
a
major
component
of
plastic
waste.
Enzymatic
PET
hydrolysis
the
most
ecofriendly
recycling
technology.
The
biorecycling
waste
requires
complete
depolymerization
to
and
ethylene
glycol.
history
enzymatic
has
revealed
two
critical
issues
for
industrial
PET:
industrially
available
hydrolases
pretreatment
make
it
susceptible
full
hydrolysis.
As
none
wild-type
enzymes
can
satisfy
requirements
industrialization,
various
mutational
improvements
have
been
performed,
through
classical
technology
state-of-the-art
computational/machine-learning
Recent
engineering
studies
on
brought
new
insight
that
flexibility
substrate-binding
groove
may
improve
efficiency
while
maintaining
sufficient
thermostability,
although
previous
focused
only
thermostability
above
glass
transition
temperature
PET.
Industrial
scheduled
be
implemented,
using
micronized
amorphous
Next
stage
must
development
efficiently
degrade
crystalline
parts
expansion
target
materials,
not
bottles
but
also
textiles,
packages,
microplastics.
This
review
discusses
current
status
hydrolases,
their
potential
applications,
profespectal
goals.
Key
points
•
thermophilic,
operation
below
70
°C
Classical
approaches
are
useful
Enzyme
activity
expected
future
Graphical
Nature Communications,
Год журнала:
2024,
Номер
15(1)
Опубликована: Фев. 15, 2024
Abstract
Biotechnological
plastic
recycling
has
emerged
as
a
suitable
option
for
addressing
the
pollution
crisis.
A
major
breakthrough
in
biodegradation
of
poly(ethylene
terephthalate)
(PET)
is
achieved
by
using
LCC
variant,
which
permits
90%
conversion
at
an
industrial
level.
Despite
achievements,
its
applications
have
been
hampered
remaining
10%
nonbiodegradable
PET.
Herein,
we
address
current
challenges
employing
computational
strategy
to
engineer
hydrolase
from
bacterium
HR29.
The
redesigned
TurboPETase,
outperforms
other
well-known
PET
hydrolases.
Nearly
complete
depolymerization
accomplished
8
h
solids
loading
200
g
kg
−1
.
Kinetic
and
structural
analysis
suggest
that
improved
performance
may
be
attributed
more
flexible
PET-binding
groove
facilitates
targeting
specific
attack
sites.
Collectively,
our
results
constitute
significant
advance
understanding
engineering
industrially
applicable
polyester
hydrolases,
provide
guidance
further
efforts
on
polymer
types.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(1), С. 593 - 593
Опубликована: Янв. 2, 2024
Plastic
production
has
increased
dramatically,
leading
to
accumulated
plastic
waste
in
the
ocean.
Marine
plastics
can
be
broken
down
into
microplastics
(<5
mm)
by
sunlight,
machinery,
and
pressure.
The
accumulation
of
organisms
release
additives
adversely
affect
health
marine
organisms.
Biodegradation
is
one
way
address
pollution
an
environmentally
friendly
manner.
microorganisms
more
adapted
fluctuating
environmental
conditions
such
as
salinity,
temperature,
pH,
pressure
compared
with
terrestrial
microorganisms,
providing
new
opportunities
pollution.
Pseudomonadota
(Proteobacteria),
Bacteroidota
(Bacteroidetes),
Bacillota
(Firmicutes),
Cyanobacteria
were
frequently
found
on
biofilms
may
degrade
plastics.
Currently,
diverse
plastic-degrading
bacteria
are
being
isolated
from
environments
offshore
deep
oceanic
waters,
especially
Pseudomonas
spp.
Bacillus
Alcanivoras
Actinomycetes.
Some
fungi
algae
have
also
been
revealed
degraders.
In
this
review,
we
focused
advances
biodegradation
their
enzymes
(esterase,
cutinase,
laccase,
etc.)
involved
process
polyethylene
terephthalate
(PET),
polystyrene
(PS),
(PE),
polyvinyl
chloride
(PVC),
polypropylene
(PP)
highlighted
need
study
sea.
Nature Communications,
Год журнала:
2023,
Номер
14(1)
Опубликована: Июль 13, 2023
Abstract
Although
considerable
research
achievements
have
been
made
to
address
the
plastic
crisis
using
enzymes,
their
applications
are
limited
due
incomplete
degradation
and
low
efficiency.
Herein,
we
report
identification
subsequent
engineering
of
BHETases,
which
potential
improve
efficiency
PET
recycling
upcycling.
Two
BHETases
(ChryBHETase
BsEst)
identified
from
environment
via
enzyme
mining.
Subsequently,
mechanism-guided
barrier
is
employed
yield
two
robust
thermostable
ΔBHETases
with
up
3.5-fold
enhanced
k
cat
/K
M
than
wild-type,
followed
by
atomic
resolution
understanding.
Coupling
ΔBHETase
into
a
two-enzyme
system
overcomes
challenge
heterogeneous
product
formation
results
in
7.0-fold
improved
TPA
production
seven
state-of-the-art
hydrolases,
under
conditions
used
here.
Finally,
employ
ΔBHETase-joined
tandem
chemical-enzymatic
approach
valorize
21
commercial
post-consumed
plastics
virgin
an
example
chemical
(
p
-phthaloyl
chloride)
for
achieving
closed-loop
open-loop
ACS Catalysis,
Год журнала:
2024,
Номер
14(5), С. 3627 - 3639
Опубликована: Фев. 21, 2024
Poly(ethylene
terephthalate)
(PET)
is
the
most
abundant
polyester
plastic
and
causing
serious
environmental
pollution.
Rapid
biological
depolymerization
of
PET
waste
at
a
large
scale
requires
powerful
engineered
enzymes
with
excellent
performance.
Here,
we
designed
computational
strategy
to
analyze
ligand
affinity
energy
chains
by
molecular
docking
dynamic
protein
conformations,
named
analysis
based
on
(ADD).
After
three
rounds
engineering
assisted
ADD,
drastically
enhanced
PET-depolymerizing
activity
leaf-branch-compost
cutinase
(LCC).
The
best
variant
LCC-A2
depolymerized
>90%
pretreated,
postconsumer
into
corresponding
monomers
within
3.3
h
78
°C,
over
99%
products
was
terminal
(terephthalic
acid
ethylene
glycol),
representing
fastest
rate
reported
date
in
bioreactor
under
optimal
condition.
Structural
revealed
interesting
features
that
improved
catalytic
In
conclusion,
proposed
variants
represent
substantial
advancement
circular
economy
for
PET.
Catalysts,
Год журнала:
2025,
Номер
15(2), С. 147 - 147
Опубликована: Фев. 4, 2025
Protein
engineering
has
emerged
as
a
transformative
field
in
industrial
biotechnology,
enabling
the
optimization
of
enzymes
to
meet
stringent
demands
for
stability,
specificity,
and
efficiency.
This
review
explores
principles
methodologies
protein
engineering,
emphasizing
rational
design,
directed
evolution,
semi-rational
approaches,
recent
integration
machine
learning.
These
strategies
have
significantly
enhanced
enzyme
performance,
even
rendering
engineered
PETase
industrially
relevant.
Insights
from
PETases
underscore
potential
tackle
environmental
challenges,
such
advancing
sustainable
plastic
recycling,
paving
way
innovative
solutions
biocatalysis.
Future
directions
point
interdisciplinary
collaborations
emerging
learning
technologies
revolutionize
design.
Communications Chemistry,
Год журнала:
2023,
Номер
6(1)
Опубликована: Сен. 11, 2023
Polyethylene
terephthalate
(PET)
is
a
commodity
polymer
known
to
globally
contaminate
marine
and
terrestrial
environments.
Today,
around
80
bacterial
fungal
PET-active
enzymes
(PETases)
are
known,
originating
from
four
two
phyla.
In
contrast,
no
archaeal
enzyme
had
been
identified
degrade
PET.
Here
we
report
on
the
structural
biochemical
characterization
of
PET46
(RLI42440.1),
an
promiscuous
feruloyl
esterase
exhibiting
degradation
activity
semi-crystalline
PET
powder
comparable
IsPETase
LCC
(wildtypes),
higher
bis-,
mono-(2-hydroxyethyl)
(BHET
MHET).
The
enzyme,
found
by
sequence-based
metagenome
search,
derived
non-cultivated,
deep-sea
Candidatus
Bathyarchaeota
archaeon.
Biochemical
demonstrated
that
promiscuous,
heat-adapted
hydrolase.
Its
crystal
structure
was
solved
at
resolution
1.71
Å.
It
shares
core
alpha/beta-hydrolase
fold
with
PETases,
but
contains
unique
lid
common
in
esterases,
which
involved
substrate
binding.
Thus,
our
study
widens
currently
diversity
PET-hydrolyzing
enzymes,
demonstrating
depolymerization
plant
cell
wall-degrading
esterase.
Green Chemistry,
Год журнала:
2023,
Номер
25(18), С. 7041 - 7057
Опубликована: Янв. 1, 2023
Biocatalysts
raised
by
the
green
chemistry
and
circular
principles
can
constitute
most
important
efficient
strategy
for
achieving
many
of
17
Sustainable
Development
Goals
launched
UN.
