A Novel Afterglow Molecular Probe for Monitoring of pH and Viscosity in Infected Wounds with Two-Dimensional Signal

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Май 13, 2025

Organic afterglow materials have shown tremendous potential in the field of biomedical imaging. However, reports on small-molecule probes, particularly those with multitarget detection capabilities, remain limited. Here, we report a novel molecule probe (Hcy-Br-SO) that effectively responds to changes pH and viscosity during wound infection, based two-dimensional (2D) signal. In this design, enhancement molecular performance was achieved through engineering, underlying mechanism emission derived. Additionally, synergistic intensity Hcy-Br-SO by increase confirmed. Besides, observed could retard photoreaction process, thereby extending duration emission. Based phenomenon, transformed traditional time-dependent characteristics into measurable parameter for monitoring changes. It is noteworthy introduction time dimension not only facilitates separation signal sources but also explores application probes. To best our knowledge, first uses 2D signals (intensity half-life) monitor binocular targets. Furthermore, successfully used distinguish between normal infected wounds. This work may be useful unravel pathological mechanisms chronic wounds provide guidance intervention.

Язык: Английский

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Sterically Controlled Cyclobutane-Dioxetane Ultrabright Afterglow Nanosystem for Cyclic Therapy of Choroidal Neovascularization in Mice

Journal of the American Chemical Society, Год журнала: 2025, Номер unknown

Опубликована: Май 20, 2025

Afterglow occurring after light excitation ceases offers a safer source to the laser-activated verteporfin therapy approved by FDA for choroidal neovascularization (CNV). However, conventional afterglow molecules, especially adamantane-dioxetanes with high steric hindrance, exhibit limited chemiexcitation, restricting electron transfer and diminishing therapeutic effects. Here, we constructed ultrabright nanosystems integrating low-hindrance cyclobutane moieties into dioxetane framework. Among these substituents, benzyl oxocyclobutane-dioxetane is brightest molecule due its lowest showing 35.7 times faster relative chemiexcitation rate 59 higher intensity than adamantane-dioxetane, alongside three-order-of-magnitude increase in total emission. Consequently, at equivalent concentration, oxocyclobutane-dioxetane-based nanosystem produces nearly five more singlet oxygen free verteporfin. In CNV mouse model, cyclic treatment our reduced lesion areas 64.9%, outperforming 39.3% reduction achieved counterpart. By eliminating need laser activation, this strategy minimizes ocular damage, providing safe effective other retinal disorders.

Язык: Английский

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Enabling robust near-infrared afterglow polymers through cascade energy transfer

Chemical Engineering Journal, Год журнала: 2024, Номер 499, С. 155836 - 155836

Опубликована: Сен. 20, 2024

Язык: Английский

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Islet cell spheroids produced by a thermally sensitive scaffold: a new diabetes treatment

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Окт. 26, 2024

The primary issues in treating type 1 diabetes mellitus (T1DM) through the transplantation of healthy islets or islet β-cells are graft rejection and a lack available donors. Currently, majority approaches use cell encapsulation technology transplant replacement cells that can release insulin to address donor shortages. However, existing materials merely serve as carriers for growth. A new treatment approach T1DM could be developed by creating smart responsive material encourages formation spheroids replicate their 3D connections vivo controls aggregates. In this study, we used microfluidics create thermally sensitive porous scaffolds made poly(N-isopropyl acrylamide)/graphene oxide (PNIPAM/GO). was carefully shrunk under near-infrared light, enriched with mouse insulinoma pancreatic β (β-TC-6 cells), encapsulated, cultivated form spheroids. controlled contraction scaffold regulated from spheroids, demonstrated using glucose-stimulated assay (GSIS), enzyme-linked immunosorbent (ELISA), immunofluorescence assay. Eventually, implantation into C57BL/6 N diabetic mice enhanced therapeutic effect, potentially offering novel management T1DM.

Язык: Английский

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Single-Molecule Detection of a Terrylenediimide-Based Near-Infrared Emitter

Chemical Science, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Design and single-molecule level scrutiny of a novel NIR absorbing emitting terrylenediimide-based chromophore, TDI-TPA 4 , is reported. The energy gap law dielectric dependence are validated at ensemble domains.

Язык: Английский

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