Computer-Aided Flexible Loops Engineering of Glutamate Dehydrogenase for Asymmetric Synthesis of Chiral Pesticides l-phosphinothricin DOI
Kai Yang,

Y C Huang,

Charles Amanze

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(44), С. 24643 - 24654

Опубликована: Окт. 22, 2024

The access to the enantiopure noncanonical amino acid l-phosphinothricin (l-PPT) by applying biocatalysts is highly appealing in organic chemistry. In this study, a NADH-dependent glutamate dehydrogenase from

Язык: Английский

Enzyme engineering for biocatalysis DOI Creative Commons
Caroline E. Paul, Ulf Hanefeld, Frank Hollmann

и другие.

Molecular Catalysis, Год журнала: 2024, Номер 555, С. 113874 - 113874

Опубликована: Янв. 31, 2024

Contemporary Biocatalysis heavily relies on enzyme engineering as natural enzymes frequently lack the requisite attributes for effective organic synthesis. The inherent limitations in stability, catalytic activity, and selectivity of wild-type often hinder their suitability chemical Over past 25 years, there has been an unprecedented advancement protein tools, empowering enzymologists to customise precisely meet demands In this discussion, we delineate some most crucial techniques significance facilitating

Язык: Английский

Процитировано

11
Ground-state flavin-dependent enzymes catalyzed enantioselective radical trifluoromethylation DOI Creative Commons

Xinyu Duan,

Dong Cui,

Mengdi Wang

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 31, 2025

Язык: Английский

Процитировано

1
Decarboxylation in Natural Products Biosynthesis DOI Creative Commons
Nguyet A. Nguyen, Jacob H. Forstater, John A. McIntosh

и другие.

JACS Au, Год журнала: 2024, Номер 4(8), С. 2715 - 2745

Опубликована: Июль 25, 2024

Decarboxylation reactions are frequently found in the biosynthesis of primary and secondary metabolites. Decarboxylase enzymes responsible for these transformations operate via diverse mechanisms act on a large variety substrates, making them appealing terms biotechnological applications. This Perspective focuses occurrence decarboxylation natural product provides perspective their applications biocatalysis fine chemicals pharmaceuticals.

Язык: Английский

Процитировано

3
Reversing the Enantioselectivity of Enzymatic Dynamic Kinetic Asymmetric Transformations in the Synthesis of Fused Lactones DOI

Mingliang Shi,

Yao Yao, Xinyue Fan

и другие.

ACS Catalysis, Год журнала: 2024, Номер unknown, С. 17480 - 17488

Опубликована: Ноя. 12, 2024

The rational design of one ketoreductase into stereocomplementary variants for controlling the stereoselectivity bulky chiral molecules bearing contiguous stereocenters is highly desirable and challenging. Herein, we report protein engineering from Chryseobacterium sp. CA49 (ChKRED20) through targeted mutagenesis only two key residues (Y188 H145) located in enzyme pocket, achieving precise stereocontrol over synthesis tricyclic fused lactones (highest reversing enantioselectivity >99:1 e.r. to <1:99 e.r.). Notably, both kinetic resolution asymmetric reduction (KR-AR) dynamic transformation (DyKAT) were observed this system. In KR-AR process, ChKRED20 exclusively convert (R)- or (S)-keto esters corresponding enantio- diastereoenriched (R,S)- (S,R)-cis-lactones deliver leftover (S)- (R)-keto esters. On contrary, DyKAT unreactive configurations substrates undergo efficient equilibration via an enolization protonation–deprotonation enzymes. Computational studies are also conducted get insight origin enantioselectivity.

Язык: Английский

Процитировано

1
A chimeric peptide promotes immune surveillance of senescent cells in injury, fibrosis, tumorigenesis and aging DOI

Xinliang Ming,

Ze Yang,

Yuqiao Huang

и другие.

Nature Aging, Год журнала: 2024, Номер unknown

Опубликована: Дек. 2, 2024

Язык: Английский

Процитировано

1
Graphical Abstract: Angew. Chem. Int. Ed. 10/2024 DOI Open Access

Anna Hartmann,

Xiang Zhang, Hongbo Xu

и другие.

Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(10)

Опубликована: Фев. 25, 2024

In their Research Article (e202314112), Andreas Dreuw

Язык: Английский

Процитировано

0
Catalytic Promiscuity of Fatty Acid Photodecarboxylase Enables Stereoselective Synthesis of Chiral α‐Tetralones DOI

Weihua Xu,

Kaihao Mou,

Zhenyu Lu

и другие.

Angewandte Chemie, Год журнала: 2024, Номер unknown

Опубликована: Авг. 14, 2024

Abstract In the field of biocatalysis, discovering novel reactivity from known enzymes has been a longstanding challenge. Fatty acid photo‐decarboxylase Chlorella variabilis ( Cv FAP) drawn considerable attention as promising photoenzyme with potential green chemistry applications; however, its non‐natural rarely exploited to date. Herein we report reductive dehalogenation (deacetoxylation) FAP inspired by natural oxidative decarboxylation process, enabling stereoselective synthesis series chiral α‐substituted tetralones high yields (up 99 %) and e.r. values : 1). Mechanistic studies demonstrated that native catalyzed via mechanism involving oxidized state (FAD ox )/semiquinone sq ) redox pair an electron transfer (ET)/proton (PT) process radical termination, distinct previous reports. To our knowledge, this study represents new example promiscuity, thus expands repertoire highlights versatility in asymmetric synthesis.

Язык: Английский

Процитировано

0
Computer-Aided Flexible Loops Engineering of Glutamate Dehydrogenase for Asymmetric Synthesis of Chiral Pesticides l-phosphinothricin DOI
Kai Yang,

Y C Huang,

Charles Amanze

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(44), С. 24643 - 24654

Опубликована: Окт. 22, 2024

The access to the enantiopure noncanonical amino acid l-phosphinothricin (l-PPT) by applying biocatalysts is highly appealing in organic chemistry. In this study, a NADH-dependent glutamate dehydrogenase from

Язык: Английский

Процитировано

0