Single molecule–driven nanomotors reveal the dynamic-disordered chemomechanical transduction of active enzymes
Science Advances,
Год журнала:
2025,
Номер
11(5)
Опубликована: Янв. 31, 2025
Enzymes
facilitate
the
conversion
of
chemical
energy
into
mechanical
work
during
biochemical
reactions,
thereby
regulating
dynamic
metabolic
activity
living
systems.
However,
directly
observing
release
facilitated
by
fluctuating
individual
enzymes
remains
a
challenge,
leading
to
contentious
debate
regarding
underlying
reasons
for
this
phenomenon.
Here,
we
aim
overcome
challenge
developing
an
oscillating
nanomotor
powered
single-molecule
enzyme,
which
allows
real-time
tracking
transduction
in
enzymatic
reactions.
Through
analysis
shifts
free
profiles
within
nanomotors,
our
results
unveil
not
only
heterogeneous
patterns
enzyme
molecules
but
also
disorder
particular
over
extended
monitoring
periods.
By
exploring
six
distinct
types
provide
direct
evidence
supporting
argument
that
reaction
enthalpy
governs
release.
This
approach
has
implications
understanding
mechanism
catalysis
and
highly
efficient
nanomotors.
Язык: Английский
Mesoporous silica micro/nanomotors: A new horizon in biomedical applications and precision medicine
Farah M. El-Makaty,
Ma.Ellyza Andrea J. Ona,
Xiaomin Li
и другие.
Applied Materials Today,
Год журнала:
2025,
Номер
43, С. 102673 - 102673
Опубликована: Март 13, 2025
Micro/Nanorobots for Advanced Light‐Based Biosensing and Imaging
Advanced Functional Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 6, 2024
Abstract
Sensing
and
imaging
of
biomolecules
are
crucial
to
disease
diagnosis,
prognosis,
therapy
where
optical
techniques
have
essential
utility.
Untethered
remotely
controlled
micro/nanorobots
shown
promising
sensing
capabilities,
especially
in
complex
biological
environments.
In
this
review,
how
used
for
biosensing
while
highlighting
the
significant
developments
field
is
discussed.
Starting
done
by
exploring
colorimetric
methods
enabled
micro/nanorobots.
Significant
advancements
surface‐enhanced
Raman
spectroscopy‐integrated
reviewed.
Further,
state‐of‐the‐art
bio‐imaging
applications
at
vitro
intracellular
level
highlighted.
Novel
vivo
assisted
micro/nanorobot
sensors
examined.
Furthermore,
innovations
assessed
motion
augmentation
as
a
detection
mechanism,
with
point‐of‐care
molecular
diagnostics.
Finally,
challenges
associated
micro/nanorobots‐assisted
advanced
discussing
insights
about
potential
research
directions
rapidly
progressing
summarized.
Язык: Английский
Urease-powered nanomotor probe for PCA3 ultrasensitive detection in human urine and controllable imaging in live cells
Sensors and Actuators B Chemical,
Год журнала:
2024,
Номер
unknown, С. 137051 - 137051
Опубликована: Ноя. 1, 2024
Язык: Английский
Recent advances in the development of tumor microenvironment-activatable nanomotors for deep tumor penetration
Qianyang Jiang,
Jiahuan He,
Hairui Zhang
и другие.
Materials Today Bio,
Год журнала:
2024,
Номер
27, С. 101119 - 101119
Опубликована: Июнь 11, 2024
Cancer
represents
a
significant
threat
to
human
health,
with
the
use
of
traditional
chemotherapy
drugs
being
limited
by
their
harsh
side
effects.
Tumor-targeted
nanocarriers
have
emerged
as
promising
solution
this
problem,
they
can
deliver
directly
tumor
site,
improving
drug
effectiveness
and
reducing
adverse
However,
efficacy
most
nanomedicines
is
hindered
poor
penetration
into
solid
tumors.
Nanomotors,
capable
converting
various
forms
energy
mechanical
for
self-propelled
movement,
offer
potential
enhancing
delivery
deep
regions.
External
force-driven
nanomotors,
such
those
powered
magnetic
fields
or
ultrasound,
provide
precise
control
but
often
necessitate
bulky
costly
external
equipment.
Bio-driven
propelled
sperm,
macrophages,
bacteria,
utilize
biological
molecules
self-propulsion
are
well-suited
physiological
environment.
constrained
lifespan,
inadequate
speed,
immune
responses.
To
address
these
issues,
nanomotors
been
engineered
propel
themselves
forward
catalyzing
intrinsic
"fuel"
in
microenvironment.
This
mechanism
facilitates
through
barriers,
allowing
them
reach
regions
targeted
delivery.
In
regard,
article
provides
review
microenvironment-activatable
(fueled
hydrogen
peroxide,
urea,
arginine),
discusses
prospects
challenges
clinical
translation,
aiming
new
insights
safe,
efficient,
treatment
cancer
therapy.
Язык: Английский
Probing Phoretic Transport of Oxidative Enzyme-Bound Zn(II)-Metallomicelle in Adenosine Triphosphate Gradient via a Spatially Relocated Biocatalytic Zone
Langmuir,
Год журнала:
2024,
Номер
40(36), С. 18906 - 18916
Опубликована: Авг. 27, 2024
Although
cellular
transport
machinery
is
mostly
ATP-driven
and
ATPase-dependent,
there
has
been
a
recent
surge
in
understanding
colloidal
processes
relying
on
nonspecific
physical
interaction
with
biologically
significant
small
molecules.
Herein,
we
probe
the
phoretic
behavior
of
biocolloid
[composed
Zn(II)-coordinated
metallomicelle
enzymes
horseradish
peroxidase
(HRP)
glucose
oxidase
(GOx)]
when
exposed
to
concentration
gradient
ATP
under
microfluidic
conditions.
Simultaneously,
demonstrate
that
an
ATP-independent
oxidative
biocatalytic
product
formation
zone
can
be
modulated
presence
(glucose
+
ATP)
gradient.
We
report
both
directionality
extent
tuned
by
changing
This
diffusiophoretic
mobility
submicrometer
biocolloidal
object
for
spatial
transposition
signifies
ATP-mediated
functional
transportation
without
involvement
ATPase.
Additionally,
ability
analyze
channels
using
enzymatic
fluorescent
product-forming
reaction
could
new
nanobiotechnological
tool
catalytic
patterning
processes.
believe
this
result
will
inspire
further
studies
realization
elusive
biological
target-specific
delivery
vehicles,
considering
omnipresence
ATP-gradient
across
cell.
Язык: Английский