Mining coral-derived terpene synthases and mechanistic studies of the coral biflorane synthase
Science Advances,
Год журнала:
2025,
Номер
11(9)
Опубликована: Фев. 26, 2025
Biflorane
diterpenoids
are
unique
natural
products
often
seen
in
marine
animals.
Recent
studies
have
reported
a
small
number
of
biflorane
synthases.
However,
the
catalytic
mechanism
and
structural
basis
for
formation
remain
unclear.
To
address
these
issues,
we
conducted
genome
mining
terpene
synthases
from
sea
whip
coral
Paramuricea
clavata
,
resulting
discovery
synthase
Pc
TS1.
We
performed
series
isotope
labeling,
crystallography,
quantum
mechanics/molecular
mechanics
calculations,
mutagenesis
toward
TS1
to
investigate
mechanism.
Isotopic
labeling
studies,
together
with
elucidate
cascade
1,10-cyclization,
1,3-hydride
shift,
1,6-cyclization,
1,2-hydride
2,6-cyclization,
cyclopropane
ring
opening,
deprotonation
by
generated
pyrophosphate,
forming
scaffold.
Crystallography,
mechanics,
confirmed
produced
different
scaffolds.
Our
work
demonstrated
formation,
elucidated
second
crystal
structure
synthase,
realized
skeleton
expansion.
Язык: Английский
From Monocyclization to Pentacyclization: A Versatile Plant Cyclase Produces Diverse Sesterterpenes with Anti‐Liver Fibrosis Potential
Advanced Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 10, 2025
Abstract
A
prolific
multi‐product
sesterterpene
synthase
CbTPS1
is
characterized
from
the
medicinal
Brassicaceae
plant
Capsella
bursa‐pastoris
.
Twenty
different
sesterterpenes
including
16
undescribed
compounds,
possessing
10
mono‐/di‐/tri‐/tetra‐/penta‐carbocyclic
skeletons,
unique
15‐membered
macrocyclic
and
24(15→14)‐
abeo
‐capbuane
scaffolds,
are
isolated
structurally
elucidated
engineered
Escherichia
coli
strains
expressing
CbTPS1.
Site‐directed
mutagenesis
assisted
by
molecular
dynamics
simulations
resulted
in
variant
L354
M
with
up
to
13.2‐fold
increased
production.
These
diverse
products
suggest
a
comprehensive
cyclization
mechanism
for
provide
compelling
evidence
initial
of
geranylfarnesyl
diphosphate
via
crucial
monocyclic
carbocation.
The
activities
these
against
liver
fibrosis
inferred
inhibition
transforming
growth
factor‐β/Smad
signaling
pathway
collagen
synthesis.
findings
greatly
expand
chemical
space
biological
functions
new
insights
into
catalytic
terpene
synthases.
Язык: Английский
Review of the Structural Characteristics and Biological Activities of Tricholoma Secondary Metabolites (2018–2023)
Molecules,
Год журнала:
2024,
Номер
29(19), С. 4719 - 4719
Опубликована: Окт. 5, 2024
are
significant
medicinal
and
edible
mushrooms
within
Basidiomycota.
Known
for
their
various
properties
such
as
anti-tumor,
immune
regulation,
antioxidant
effects,
they
regarded
worldwide
health
foods
of
the
21st
century.
Язык: Английский
Hot off the press
Natural Product Reports,
Год журнала:
2024,
Номер
41(8), С. 1214 - 1218
Опубликована: Янв. 1, 2024
A
personal
selection
of
32
recent
papers
is
presented
covering
various
aspects
current
developments
in
bioorganic
chemistry
and
novel
natural
products
such
as
nitidane
from
Язык: Английский
Terpestacin and its derivatives: bioactivities and syntheses
Chemistry & Biodiversity,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 25, 2024
Terpestacin
(1),
fusaproliferin
(2),
and
their
derivatives
are
a
class
of
sesterterpenes
featured
by
trans-fused
5/15-membered
ring
skeleton.
There
45
natural
products
(1,
2,
4-27,
65-83)
isolated
from
various
wild
fungi
(Fusarium
sp.,
Bipolaris
sorokiniana,
Arthrinium
etc.)
or
genetic
mutants
via
biosynthetic
gene
clusters
mining,
37
(28-64)
produced
semi-synthetic
modifications.
These
compounds
show
diverse
range
important
bioactivities
such
as
antivirus,
antimicrobial,
cytotoxic,
phytotoxic,
anti-flammatory,
brine
shrimp
lethal
activities.
To
date,
two
racemic
five
enantioselective
chemical
total
syntheses
1
(including
2
isomers)
have
been
developed.
Over
the
past
decade,
number
mutants,
along
with
encoding
enzymes
responsible
for
producing
terpestacin
its
derivatives,
also
identified.
This
review
covers
literature
year
1993,
when
was
firstly
discovered,
to
May
2024,
focusing
on
isomers.
Язык: Английский