Simultaneously Achieving Lipid-Droplet-Location and Activity-Based Fluorogenic Sensing of Hydrogen Peroxide by Dicyanofuran-Fused Coumarin Derivatives
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 15, 2025
Activity-based
sensing
of
hydrogen
peroxide
(H2O2)
in
specific
organelles
interest
is
significant
for
deciphering
its
physiological
functions
at
the
subcellular
level.
However,
currently
reported
H2O2-responsive
moieties
lack
organelle
targetability,
necessitating
an
auxiliary
navigation
to
direct
probe
molecule
targeted
cellular
local
region.
Herein,
we
propose
economical
strategy
by
integrating
a
dual-functional
moiety
that
combines
molecular
recognition
and
targeting.
By
exploiting
oxidation
reaction
dicyanofuran,
two
novel
H2O2-activatable
probes
capable
targeting
lipid
droplets
(LDs)
were
rationally
designed
based
on
coumarin
scaffold.
Notably,
dicyanofuran-fused
derivative
FC1
could
effectively
image
endogenous
exogenous
H2O2
LDs
living
cells
has
been
successfully
utilized
distinguish
cancer
from
normal
leveraging
synergistic
combination
elevated
levels
LD
abundance.
Our
work
not
only
presents
new
activity-based
mechanism
but
also
provides
versatile
approach
development
fluorescent
future.
Язык: Английский
Phenyl Radical-Mediated Fluorogenic Cyclization for Specific Detection of Peroxynitrite
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 27, 2025
Peroxynitrite
(ONOO–),
a
biological
oxidizing
and
nitrating
species
responsible
for
post-translational
modification
of
cellular
proteins,
has
been
implicated
in
numerous
pathologies
carrying
an
inflammatory
component.
Specific
detection
ONOO–
systems
remains
challenge,
boronates
are
regarded
as
the
most
promising
class
probes
quantitation
ONOO–.
Oxidation
boronate
by
results
formation
minor
ONOO–-specific
products
via
pathway
involving
phenyl
radical-type
intermediate,
addition
to
major
phenolic
product.
Here,
we
report
fluorogenic
cyclization
phenyl-type
radical
formed
during
oxidation
probe
ONOO–,
with
production
fluorescent
product,
propose
new
approach
specific
based
on
this
observation.
We
characterized
kinetics
stoichiometry
reaction
benzophenone-2-boronic
acid
identified
2-hydroxybenzophenone
product
fluorenone
(FLN)
2-nitrobenzophenone
products.
Hydrogen
peroxide
neither
alone
nor
presence
myeloperoxidase
nitrite
produces
FLN
or
2-nitrobenzophenone.
can
be
selectively
detected
using
fluorescence
spectroscopy,
providing
chemical
principle
development
next-generation
noninvasive,
fluorescence-based
Fluorescence-based
monitoring
was
successfully
applied
generated
situ
from
decomposition
SIN-1,
thermal
source
superoxide
anion
nitric
oxide.
Язык: Английский
A novel activation-triggered-immobilizing fluorescent probe for real-time tracking of mitochondrial ONOOˉ with high fidelity
Sensors and Actuators B Chemical,
Год журнала:
2025,
Номер
unknown, С. 137774 - 137774
Опубликована: Апрель 1, 2025
Язык: Английский
Smartphone-Integrated Dual-Mode Fluorescence-Colorimetric Sensor Based on Aggregation-Induced Emission for Peroxynitrite Detection
Опубликована: Янв. 1, 2025
Язык: Английский
Screening Anti-Parkinson’s Disease Drugs in Living Mouse Brains via a Peroxynitrite-Activated Fluorescent Probe
Chemical & Biomedical Imaging,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 20, 2024
Screening
anti-Parkinson's
disease
(PD)
drugs
at
in
vivo
brain
level
is
imperative
for
managing
PD
yet
currently
remains
unaccomplished.
Peroxynitrite
(ONOO–)
has
been
implicated
progression.
Thus,
developing
ONOO–-based
imaging
tools
anti-PD
drug
screening
holds
promise
early
prognosis
and
treatment
of
PD.
Consequently,
a
near-infrared
(NIR)
fluorescence
probe,
BOB-Cl-PN,
with
high
specificity,
good
sensitivity
(LOD
=
24
nM),
rapid
response
(<60
s),
was
devised
to
investigate
ONOO–
relationships.
Utilizing
NIR
imaging,
BOB-Cl-PN
effectively
monitored
fluctuations
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
(MPTP)-induced
cell
models,
establishing
cellular
throughput
(cHTS)
system
drugs.
In
live
animal
BOB-Cl-PN's
ability
penetrate
the
blood–brain
barrier
enabled
flux
mouse
brains.
Moreover,
served
as
an
contrast
potential
traditional
Chinese
medicines
therapy,
identifying
fisetin
having
best
therapeutic
index
among
10
medicines.
This
study
constructs
sensitive,
efficient
monitoring
dynamics
brains
provides
valuable
platform
Язык: Английский