An Enzyme-Triggered Au–Se Nanodevice for Precise Imaging of MicroRNA DOI
Ruyue Wei, Shuqi Wang,

Yingbo Pan

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 15, 2025

In situ imaging of microRNA (miRNA) in tumor cells is vital for clinical diagnosis and pathological research. However, achieving high-precision always limited by undesirable background signals. Herein, we introduced a gold-selenium-based nanodevice (AuSeND) high-fidelity miRNA via enzyme-triggered catalytic hairpin assembly (CHA). This system employs an enzyme-activatable CHA circuit, constructed extending short tail at the 3' end H1 with apurinic/apyrimidinic (AP) site. The circuit components are connected to surface AuNPs Au-Se bonds, forming nanodevice. It remains inactive normal cells, while activated endonuclease 1 (APE1) cytoplasm, generating fluorescence signal under stimulation imaging. developed AuSeND enables cancer cell-selective improves signal-to-noise ratio combining high stability bond specific regulation APE1 enzyme, offering strong potential diagnostic applications.

Язык: Английский

Emerging Trends in DNA Nanotechnology-Enabled Cell Surface Engineering DOI Creative Commons
Fan Xiao,

Xuechu Shen,

Wenqi Tang

и другие.

JACS Au, Год журнала: 2025, Номер 5(2), С. 550 - 570

Опубликована: Фев. 6, 2025

Cell surface engineering is a rapidly advancing field, pivotal for understanding cellular physiology and driving innovations in biomedical applications. In this regard, DNA nanotechnology offers unprecedented potential precisely manipulating functionalizing cell surfaces by virtue of its inherent programmability versatile functionalities. Herein, Perspective provides comprehensive overview emerging trends engineering, focusing on key nanostructure-based tools, their roles regulating physiological processes, We first discuss the strategies integrating molecules onto surfaces, including attachment oligonucleotides higher-order nanostructure. Second, we summarize impact DNA-based various such as membrane protein degradation, signaling transduction, intercellular communication, construction artificial components. Third, highlight applications DNA-engineered targeted therapies cancer inflammation, well capture/protection diagnostic detection. Finally, address challenges future directions nanotechnology-based engineering. This aims to provide valuable insights rational design contributing development precise personalized medicine.

Язык: Английский

Процитировано

1
NIR-Triggered Programmable Nanomotor with H2S and NO Generation for Cascading Oncotherapy by Three-Pronged Reinforcing ICD DOI Creative Commons
Jinlong Zhang, Quan Jing,

L. Yuan

и другие.

Materials Today Bio, Год журнала: 2025, Номер 31, С. 101540 - 101540

Опубликована: Фев. 3, 2025

Язык: Английский

Процитировано

0
Programmable Split DNAzyme Modulators via Allosteric Cooperative Activation for mRNA Electrochemiluminescence Biosensing DOI

Liu-Qing Tan,

Weijia Zeng,

Qiaolin Chen

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

DNAzymes, known for their programmability, stability, and cost-effectiveness, are powerful tools signal transduction in complex biological systems. However, application responding to target effectors is often hindered by limited catalytic efficiency susceptibility unintended activation. Here we propose an allosteric cooperative activation strategy program a split DNAzyme modulator (STATER) that enables sensitive accurate electrochemiluminescence (ECL) biosensing of interleukin-6 (IL-6) mRNA. Our design features STATER leverages DNA tetrahedron as central scaffold, equipped with two pairs T-shaped hairpin probes (TP) helper (HP). Specifically, the TP contains apurinic/apyrimidinic endonuclease 1 (APE1) recognition sites, IL-6 mRNA region, partzyme fragment, while HP corresponding paired fragment. Unlike conventional modulators rely on single effector activation, integrates mechanism, which ensures all preblocked components synergistically activated assembled within confined space, facilitating rapid specific reconstruction DNAzyme's active domain. Furthermore, upon APE1 mRNA, inactive partzymes undergo assembly via toehold exchange displacement reaction, switching cleavage reactivity STATER. This mechanism establishment threshold thereby minimizing nonspecific scenarios. studies demonstrate exhibits outstanding sensitivity selectivity detection using supramolecular gold nanoclusters network-based ECL platform. The biosensor provides linear span from × 10–13 10–7 M, limit low 3.26 10–14 highlighting STATER's potential detecting various analytes

Язык: Английский

Процитировано

0
An Enzyme-Triggered Au–Se Nanodevice for Precise Imaging of MicroRNA DOI
Ruyue Wei, Shuqi Wang,

Yingbo Pan

и другие.

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 15, 2025

In situ imaging of microRNA (miRNA) in tumor cells is vital for clinical diagnosis and pathological research. However, achieving high-precision always limited by undesirable background signals. Herein, we introduced a gold-selenium-based nanodevice (AuSeND) high-fidelity miRNA via enzyme-triggered catalytic hairpin assembly (CHA). This system employs an enzyme-activatable CHA circuit, constructed extending short tail at the 3' end H1 with apurinic/apyrimidinic (AP) site. The circuit components are connected to surface AuNPs Au-Se bonds, forming nanodevice. It remains inactive normal cells, while activated endonuclease 1 (APE1) cytoplasm, generating fluorescence signal under stimulation imaging. developed AuSeND enables cancer cell-selective improves signal-to-noise ratio combining high stability bond specific regulation APE1 enzyme, offering strong potential diagnostic applications.

Язык: Английский

Процитировано

0