A Metal–Organic Hybrid Composed of Dual Quenching Cofactors as a Nanoquencher for the Fluorescent Determination of Protease Caspase-3 DOI Creative Commons

Fengli Gao,

Lin Liu, Cheng-Ru He

и другие.

Biosensors, Год журнала: 2025, Номер 15(6), С. 354 - 354

Опубликована: Июнь 4, 2025

Nanoquenchers with a single quenching cofactor exhibit limited fluorescence efficiency. In this work, metal–organic hybrid dual cofactors (Cu2+ and pyrroloquinoline quinone or PQQ) was prepared by metal-coordinated assembly used as nanoquencher for protease assay enhanced The peptide substrate an oligohistidine (His6) tag labeled fluorophore. Caspase-3 determined example. attached onto the surface of Cu-PQQ metal coordination interaction between unsaturated Cu2+ on nanoparticle His6 in peptide. cleavage enzymatic hydrolysis led to release fluorophore-conjugated segment from surface, thus turning fluorescence. nanoprobe determine caspase-3 linear range 0.01–5 ng/mL detection limit 7 pg/mL. Furthermore, method evaluate inhibition efficiency monitor drug-induced cell apoptosis. contrast other means immobilization, such physical adsorption covalent coupling, strategy based is simple powerful, thereby achieving assays activity lysates satisfactory result. work should be valuable design nanoquenchers multiple development novel biosensors.

Язык: Английский

NIR-Triggered Programmable Nanomotor with H2S and NO Generation for Cascading Oncotherapy by Three-Pronged Reinforcing ICD DOI Creative Commons
Jinlong Zhang, Quan Jing,

L. Yuan

и другие.

Materials Today Bio, Год журнала: 2025, Номер 31, С. 101540 - 101540

Опубликована: Фев. 3, 2025

Язык: Английский

Процитировано

1
Magnetic Microbubbles Combined with ICG-Loaded Liposomes for Synergistic Mild-Photothermal and Ferroptosis-Enhanced Photodynamic Therapy of Melanoma DOI Creative Commons

Kaifen Xiong,

Guanghong Luo,

Wei Zeng

и другие.

International Journal of Nanomedicine, Год журнала: 2025, Номер Volume 20, С. 2901 - 2921

Опубликована: Март 1, 2025

Background: Melanoma poses a significant threat to human health due the lack of effective treatment options. Previous studies have demonstrated that combination photothermal therapy (PTT) and photodynamic (PDT) can enhance therapeutic efficacy. However, conventional PTT/PDT strategies face various challenges, including complex preparation processes, potential damage healthy tissues, insufficient generation reactive oxygen species (ROS). This study aims design rational efficient strategy for melanoma explore its underlying mechanisms. Methods: We first synthesized two target materials, indocyanine green-targeted liposomes (ICG-Lips) magnetic microbubbles (MMBs), using thin-film hydration method, followed by characterization performance evaluation both materials. Subsequently, we evaluated synergistic effects mechanisms ICG-Lips combined with MMBs in through vitro experiments cellular models vivo animal models. Results: Herein, developed multifunctional system comprising MMBs. targeted delivery specific binding S100B protein on cells, while MMBs, via ultrasound (US)-induced cavitation effects, shorten uptake time cells improve efficiency. Furthermore, induces (ROS) generation. Under 808 nm laser irradiation, accumulation achieves mild (mPTT) PDT effects. The elevated temperature excessive ROS generated during these processes result glutathione (GSH) depletion, ultimately triggering ferroptosis. occurrence ferroptosis further amplifies efficacy, creating effect effectively suppresses growth. Additionally, demonstrates excellent biosafety. Conclusion: In summary, this presents novel straightforward integrates mPTT, PDT, synergistically combat melanoma, thereby laying solid foundation improving outcomes. Keywords: microbubbles, ferroptosis, mild-temperature therapy,

Язык: Английский

Процитировано

0
A cyanine fluorophore for imaging-guided tumor photodynamic and photothermal therapy under NIR-II light activation DOI
Wenkai Liu,

Chengyuan Lv,

Yanxian Hou

и другие.

Science China Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Язык: Английский

Процитировано

0
Nano-immunomodulator amplifies STING activation in tumor-associated macrophages for cancer immunotherapy DOI

Shuang Liang,

Haiyan Ma, Yue Liu

и другие.

Journal of Controlled Release, Год журнала: 2025, Номер unknown, С. 113846 - 113846

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
Harnessing Photo‐Energy Conversion in Nanomaterials for Precision Theranostics DOI Creative Commons
Jingyu Shi, Yadi Fan, Qin Zhang

и другие.

Advanced Materials, Год журнала: 2025, Номер unknown

Опубликована: Май 16, 2025

Abstract The rapidly advancing field of theranostics aims to integrate therapeutic and diagnostic functionalities into a single platform for precision medicine, enabling the simultaneous treatment monitoring diseases. Photo‐energy conversion‐based nanomaterials have emerged as versatile that utilizes unique properties light activate with high spatial temporal precision. This review provides comprehensive overview recent developments in photo‐energy conversion using nanomaterials, highlighting their applications disease theranostics. discussion begins by exploring fundamental principles including types materials used various light‐triggered mechanisms, such photoluminescence, photothermal, photoelectric, photoacoustic, photo‐triggered SERS, photodynamic processes. Following this, delves broad spectrum emphasizing role diagnosis major diseases, cancer, neurodegenerative disorders, retinal degeneration, osteoarthritis. Finally, challenges opportunities technologies are discussed, aiming advance personalized medicine.

Язык: Английский

Процитировано

0
Dendrimer-Entrapped CuPt Bimetallic Nanozymes for Tumor Microenvironment-Regulated Photothermal/Catalytic Therapy DOI

Suojun Lu,

Aojie Li,

Haoyu Huang

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Май 19, 2025

Noble metal nanozymes have attracted extensive attention in tumor therapy due to their multiple enzyme-like activities for catalytic and unique optical properties photothermal (PTT). However, therapeutic efficiency may be compromised by the intricate microenvironment (TME). Herein, copper-platinum (CuPt) bimetallic were synthesized entrapped poly(amidoamine) dendrimers, with p-carboxybenzenesulfonamide (BS, a carbonic anhydrase IX (CA IX) inhibitor) modified on surface lonidamine (Lon) loaded inside regulate TME enhanced PTT. The formed CPL@G5-BS could specifically target CA IX-overexpressed cells activate cascade reaction continuous •OH generation peroxidase-like property, O2 supply catalase-like H2O2 replenishing superoxide dismutase-like thereby alleviating hypoxia achieving chemodynamic (CDT). In TME, BS-mediated inhibition would normalize extracellular pH suppress metastasis while reducing intracellular boost efficiency, Lon-mediated mitochondrial respiration energy metabolic disruption elevate oxygen accumulation downregulate heat shock protein (HSP) expression, further enhancing PTT efficacy. Meanwhile, excellent performance of amplify multienzyme activities, Cu2+-mediated glutathione depletion improved CDT efficiency. Overall, can efficiently inhibit growth TME-regulated

Язык: Английский

Процитировано

0
Polydopamine‐Integrated Nanomedicine through Dual Metabolic Intervention with Enhanced Photothermal Therapy against Triple‐Negative Breast Cancer DOI

Chenlu Xu,

Yujuan Gao, Xiaoyu Zhang

и другие.

Advanced Therapeutics, Год журнала: 2025, Номер unknown

Опубликована: Май 22, 2025

Abstract Combining metabolic therapy with photothermal (PTT) shows promise for the treatment of triple‐negative breast cancer (TNBC). However, a strategy is still required to effectively overcome adaptation TNBC thermotolerance during thermotherapy, thereby enhancing therapeutic outcomes. In this study, polydopamine‐integrated nanomedicine designed, glucose oxidase (GOx) chemically conjugated its surface and curcumin (Cur)‐loaded calcium phosphate (CaP) shell. The outer CaP layer disintegrates in response acidic environment tumor cells, releasing ions efflux inhibitor Cur, which causes mitochondrial functional disruption by inducing overload. Concurrently, GOx consumes intracellular inhibit glycolysis. dual intervention metabolism glycolysis serves counteract adaptations TNBC, blocking pathways energy supply, results 56.61% reduction ATP. addition, inhibition ATP production regulation can downregulate expression heat shock proteins (HSPs) sensitized PTT, HSP70 level HSP90 are downregulated 25.68% 41.89%, respectively. This study provides new insights into combining PTT TNBC.

Язык: Английский

Процитировано

0
A Metal–Organic Hybrid Composed of Dual Quenching Cofactors as a Nanoquencher for the Fluorescent Determination of Protease Caspase-3 DOI Creative Commons

Fengli Gao,

Lin Liu, Cheng-Ru He

и другие.

Biosensors, Год журнала: 2025, Номер 15(6), С. 354 - 354

Опубликована: Июнь 4, 2025

Nanoquenchers with a single quenching cofactor exhibit limited fluorescence efficiency. In this work, metal–organic hybrid dual cofactors (Cu2+ and pyrroloquinoline quinone or PQQ) was prepared by metal-coordinated assembly used as nanoquencher for protease assay enhanced The peptide substrate an oligohistidine (His6) tag labeled fluorophore. Caspase-3 determined example. attached onto the surface of Cu-PQQ metal coordination interaction between unsaturated Cu2+ on nanoparticle His6 in peptide. cleavage enzymatic hydrolysis led to release fluorophore-conjugated segment from surface, thus turning fluorescence. nanoprobe determine caspase-3 linear range 0.01–5 ng/mL detection limit 7 pg/mL. Furthermore, method evaluate inhibition efficiency monitor drug-induced cell apoptosis. contrast other means immobilization, such physical adsorption covalent coupling, strategy based is simple powerful, thereby achieving assays activity lysates satisfactory result. work should be valuable design nanoquenchers multiple development novel biosensors.

Язык: Английский

Процитировано

0