CRISPR/Cas12a-based biosensors for environmental monitoring and diagnostics

Environmental Technology & Innovation, Год журнала: 2024, Номер 34, С. 103625 - 103625

Опубликована: Апрель 4, 2024

Contaminants, such as nucleic acids or toxic small molecules, threaten both human health and ecosystems when they infiltrate the environment. The precise highly sensitive identification of contaminants holds paramount importance across diverse domains, including safeguarding food integrity, facilitating clinical diagnostics, monitoring environmental conditions. Traditional methodologies, encompassing spectroscopy, chromatography, sequencing, metagenomics, have conventionally served pivotal roles in detection processes. Nevertheless, these methods encountered recurring challenges related to sensitivity, specificity, portability. This review focuses on groundbreaking CRISPR/Cas12-based biosensors. These biosensors leverage incredible precision programmability CRISPR/Cas system recognize specific targets. Here, we comprehensively assess fundamental mechanisms that enable detection, ranging from guide RNA design collateral cleavage. versatility CRISPR/Cas12 becomes evident through their applications. applications encompass medical safety, monitoring. transition conventional ultimately represents a significant milestone contaminant detection. By incorporating molecular biology, nanotechnology, bioinformatics, potential reshape landscape water CRIPSR-Cas diagnostics is transformative technology paves way for safer healthier future environment life.

Язык: Английский

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Applications of surface‐enhanced Raman spectroscopy in environmental detection

Analytical Science Advances, Год журнала: 2022, Номер 3(3-4), С. 113 - 145

Опубликована: Март 23, 2022

As the human population grows, anthropogenic impacts from various agricultural and industrial processes produce unwanted contaminants in environment. The accurate, sensitive rapid detection of such is vital for health safety. Surface-enhanced Raman spectroscopy (SERS) a valuable analytical tool with wide applications environmental contaminant monitoring. aim this review to summarize recent advancements within SERS research as it applies detection, focus on published or accessible January 2021 through December including early-access publications. Our goal provide breadth information that can be used background knowledge field, well inform encourage further development techniques protecting quality Specifically, we highlight characteristics effective nanosubstrates, explore methods inorganic, organic, biological heavy metals, pharmaceuticals, plastic particles, synthetic dyes, pesticides, viruses, bacteria mycotoxins. We also discuss current limitations technologies propose several avenues future investigation. researchers fill identified gaps so implemented real-world environment more effectively efficiently, ultimately providing reliable timely data help make science-based strategies policies protect safety public health.

Язык: Английский

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MXene-based aptasensor for the detection of aflatoxin in food and agricultural products

Environmental Pollution, Год журнала: 2022, Номер 316, С. 120695 - 120695

Опубликована: Ноя. 21, 2022

Язык: Английский

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Polyacrylonitrile/N-doped graphene quantum dots nanocomposite activity as SERS nanosensors for detection of methylene blue

Materials Today Communications, Год журнала: 2023, Номер 36, С. 106860 - 106860

Опубликована: Авг. 1, 2023

Язык: Английский

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Advanced visual sensing techniques for on-site detection of pesticide residue in water environments

Heliyon, Год журнала: 2023, Номер 9(3), С. e13986 - e13986

Опубликована: Фев. 24, 2023

Pesticide usage has increased to fulfil agricultural demand. Pesticides such as organophosphorus pesticides (OPPs) are ubiquitous in world food production. Their widespread unavoidable detrimental consequences for humans, wildlife, water, and soil environments. Hence, the development of more convenient efficient pesticide residue (PR) detection methods is paramount importance. Visual detecting approaches have acquired a lot interest among different sensing systems due inherent advantages terms simplicity, speed, sensitivity, eco-friendliness. Furthermore, various detections been proven enable real-life PR surveillance environment water. Fluorometric (FL), colourimetric (CL), enzyme-inhibition (EI) techniques emerged viable options. These technologies do not need complex operating processes or specialist equipment, simple colour change allows visual monitoring result. on-site water environments discussed this paper. This paper further reviews prior research on integration CL, FL, EI-based with nanoparticles (NPs), quantum dots (QDs), metal-organic frameworks (MOFs). Smartphone PRs also reviewed. Finally, conventional nanoparticle (NPs) based strategies compared.

Язык: Английский

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Machine Learning-Assisted Surface-Enhanced Raman Spectroscopy Detection for Environmental Applications: A Review

Environmental Science & Technology, Год журнала: 2024, Номер 58(47), С. 20830 - 20848

Опубликована: Ноя. 13, 2024

Surface-enhanced Raman spectroscopy (SERS) has gained significant attention for its ability to detect environmental contaminants with high sensitivity and specificity. The cost-effectiveness potential portability of the technique further enhance appeal widespread application. However, challenges such as management voluminous quantities high-dimensional data, capacity low-concentration targets in presence interferents, navigation complex relationships arising from overlapping spectral peaks have emerged. In response, there is a growing trend toward use machine learning (ML) approaches that encompass multivariate tools effective SERS data analysis. This comprehensive review delves into detailed steps needed be considered when applying ML techniques Additionally, we explored range applications where different were integrated detection pathogens (in)organic pollutants samples. We sought comprehend intricate considerations benefits associated these contexts. explores future synergizing real-world applications.

Язык: Английский

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Enhanced Charge Transfer and Coupled Resonance in Ni-doped sub-stoichiometric Tungsten Oxide Nanostructure for Plasmon-free SERS Sensing

Materials Today Nano, Год журнала: 2025, Номер unknown, С. 100593 - 100593

Опубликована: Фев. 1, 2025

Язык: Английский

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Graphene-based SERS-active substrates for environmental detection: nanoarchitectonics and applications

Journal of Materials Science, Год журнала: 2025, Номер unknown

Опубликована: Фев. 28, 2025

Язык: Английский

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Advanced microplastic monitoring using Raman spectroscopy with a combination of nanostructure-based substrates

Journal of nanostructure in chemistry, Год журнала: 2022, Номер 12(5), С. 865 - 888

Опубликована: Июнь 18, 2022

Язык: Английский

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Screening for pesticide residues in cocoa (Theobroma cacao L.) by portable infrared spectroscopy

Talanta, Год журнала: 2023, Номер 257, С. 124386 - 124386

Опубликована: Фев. 18, 2023

Язык: Английский

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