Detecting Environmental DNA From Crested and Smooth Newts—Not as Straight Forward as Filtering a Drop of Water DOI Open Access
Annette Taugbøl, Frode Fossøy, Børre Kind Dervo

и другие.

Aquatic Conservation Marine and Freshwater Ecosystems, Год журнала: 2025, Номер 35(3)

Опубликована: Март 1, 2025

ABSTRACT Environmental DNA methodologies are constantly being developed and optimized for specific purposes. This paper summarizes 3 years of water‐sampling filtering method development, with the aim to reliably detect quantify eDNA from great crested newts smooth established monitoring ponds, using species‐specific assays droplet digital PCR (ddPCR). Both newt species were caught in traps all ponds during three sampling years. For first year, water was collected separate positions within each pond filtered individually through a 0.45‐μm PES filter. Overall, replicates resulted 26% false negatives, including where only one‐third samples positive. Positive results sites also showed high variance concentration (the highest across filters nine ddPCRs: 1987 ± 1789). second subsamples different mixed before two filters. approach reduced overall variation batch variance: 7355 4147). However, few did still not (9%–10%). third test potential effects stratification seasonal on probability detection, both surface layer 30 cm below four five times main mating period. In addition, filter type changed 2.0‐μm glass fibre increase volume. Despite average 1 L more compared this produced higher number negatives. Optimizing methods season is needed applying managemental monitoring.

Язык: Английский

Detecting Environmental DNA From Crested and Smooth Newts—Not as Straight Forward as Filtering a Drop of Water DOI Open Access
Annette Taugbøl, Frode Fossøy, Børre Kind Dervo

и другие.

Aquatic Conservation Marine and Freshwater Ecosystems, Год журнала: 2025, Номер 35(3)

Опубликована: Март 1, 2025

ABSTRACT Environmental DNA methodologies are constantly being developed and optimized for specific purposes. This paper summarizes 3 years of water‐sampling filtering method development, with the aim to reliably detect quantify eDNA from great crested newts smooth established monitoring ponds, using species‐specific assays droplet digital PCR (ddPCR). Both newt species were caught in traps all ponds during three sampling years. For first year, water was collected separate positions within each pond filtered individually through a 0.45‐μm PES filter. Overall, replicates resulted 26% false negatives, including where only one‐third samples positive. Positive results sites also showed high variance concentration (the highest across filters nine ddPCRs: 1987 ± 1789). second subsamples different mixed before two filters. approach reduced overall variation batch variance: 7355 4147). However, few did still not (9%–10%). third test potential effects stratification seasonal on probability detection, both surface layer 30 cm below four five times main mating period. In addition, filter type changed 2.0‐μm glass fibre increase volume. Despite average 1 L more compared this produced higher number negatives. Optimizing methods season is needed applying managemental monitoring.

Язык: Английский

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