Biotechnology and Applied Biochemistry,
Год журнала:
2023,
Номер
71(1), С. 17 - 27
Опубликована: Сен. 25, 2023
Abstract
Elevation
of
one
or
more
plasma
lipids,
such
as
phospholipids,
cholesterol
esters,
cholesterol,
and
triglycerides,
is
known
hyperlipidemia.
In
humans
experimental
animals,
bromelain,
the
primary
active
ingredient
isolated
from
pineapple
stems,
has
several
positive
effects,
including
anti‐tumor
growth,
anticoagulation,
anti‐inflammation.
Hence,
purpose
this
study
was
to
determine
possible
protective
impact
bromelain
on
some
metabolic
enzymes
(paraoxonase‐1,
glutathione
S
‐transferase,
reductase,
sorbitol
dehydrogenase
[SDH],
aldose
reductase
[AR],
butyrylcholinesterase
[BChE],
acetylcholinesterase
[AChE]),
activity
in
heart,
kidney,
liver
rats
with
tyloxapol‐induced
Rats
were
divided
into
three
groups:
control
group,
HL‐control
group
(tyloxapol
400
mg/kg,
i.p.
administered
group),
HL+bromelain
(group
receiving
250
mg/kg/o.d.
prior
administration
tyloxapol
i.p.).
BChE,
SDH,
AR
enzyme
activities
significantly
increased
all
tissues
compared
control,
whereas
other
studied
decreased.
Bromelain
had
a
regulatory
effect
activities.
conclusion,
these
results
prove
that
new
mediator
decreases
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Янв. 2, 2024
Abstract
Xanthine
oxidase
(XO)
is
a
crucial
enzyme
in
the
development
of
hyperuricemia
and
gout.
This
study
focuses
on
LWM
ALPM,
two
food-derived
inhibitors
XO.
We
used
molecular
docking
to
obtain
three
systems
then
conducted
200
ns
dynamics
simulations
for
Apo,
LWM,
ALPM
systems.
The
results
reveal
stronger
binding
affinity
peptide
XO,
potentially
due
increased
hydrogen
bond
formation.
Notable
changes
were
observed
XO
tunnel
upon
inhibitor
binding,
particularly
with
which
showed
thinner,
longer,
more
twisted
configuration
compared
ALPM.
highlights
importance
residue
F914
allosteric
pathway.
Methodologically,
we
utilized
perturbed
response
scan
(PRS)
based
Python,
enhancing
tools
MD
analysis.
These
findings
deepen
our
understanding
anti-XO
could
inform
food-based
therapeutics
reducing
uric
acid
levels
minimal
side
effects.
Chemistry & Biodiversity,
Год журнала:
2022,
Номер
19(8)
Опубликована: Июль 7, 2022
Abstract
In
the
current
study,
some
phenolic
compounds,
including
acteoside,
isoacteoside,
echinacoside,
and
arenarioside
purified
characterized
from
Plantago
subulata
.
These
compounds
were
tested
for
its
antioxidant
potential,
Fe
3+
Cu
2+
reductive
ability
chelating
effects.
The
inhibitory
effects
of
isolated
towards
human
carbonic
anhydrase
I
II
isoenzymes
(hCA
hCA
II),
butyrylcholinesterase
(BChE)
acetylcholinesterase
(AChE),
aldose
reductase
(AR)
α‐glycosidase
(α‐gly).
K
i
values
found
these
in
range
0.24±0.05–1.38±0.34
μM
against
I,
0.194±0.018–1.03±0.06
II,
0.043±0.01–0.154±0.02
AChE,
3.92±1.08–11.93±4.45
BChE,
0.082±0.0008–1.68±0.42
AR,
6.93±2.74–17.17±6.70
α‐glycosidase.
As
a
result,
displayed
inhibition
studied
all
metabolic
enzymes.
They
are
promising
candidates
treating
disorders
like
Alzheimer's
disease,
diabetes
mellitus,
glaucoma,
leukemia,
epilepsy.
Archiv der Pharmazie,
Год журнала:
2022,
Номер
356(4)
Опубликована: Дек. 27, 2022
New
Schiff
base-bearing
thiosemicarbazones
(1-13)
were
obtained
from
4-hydroxy-3,5-dimethoxy
benzaldehyde
and
various
isocyanates.
The
structures
of
the
synthesized
molecules
elucidated
in
detail.
Density
functional
theory
calculations
also
performed
to
determine
spectroscopic
properties
compounds.
Moreover,
enzyme
inhibition
activities
these
compounds
investigated.
They
showed
highly
potent
effects
on
acetylcholinesterase
(AChE)
human
carbonic
anhydrases
(hCAs)
(KI
values
are
range
51.11
±
6.01
278.10
40.55
nM,
60.32
9.78
300.00
77.41
64.21
9.99
307.70
61.35
nM
for
AChE,
hCA
I,
II,
respectively).
In
addition,
molecular
docking
studies
performed,
confirmed
by
binding
affinities
most
derivatives.
