Investigation of Probiotic Properties of Lacticaseibacillus casei 4 N‐6 Strain Isolated From Cow Milk
Food Science & Nutrition,
Год журнала:
2025,
Номер
13(4)
Опубликована: Апрель 1, 2025
ABSTRACT
The
aim
of
the
present
study
was
to
characterize
probiotic
potential
Lacticaseibacillus
casei
4
N‐6
strain
isolated
from
cow's
milk.
For
this
purpose,
acid,
bile
salt,
pancreatin,
pepsin,
phenol,
and
lysozyme
tolerance,
co‐aggregation
auto‐aggregation
properties,
phytase
production,
antibiotic
resistance,
antibacterial
properties
were
analyzed
in
vitro.
had
relatively
poor
pepsin
tolerance.
However,
showed
a
high
lysozyme,
phenol
In
addition,
it
exhibited
moderate
with
E.
coli
good
autoaggregation.
Furthermore,
cell‐free
supernatant
Lb
.
antimicrobial
activity
against
Bacillus
cereus
(N32),
Salmonella
enteritidis
(RK‐485),
Enterococcus
faecalis
(RK‐487).
resistant
vancomycin,
teicoplanin,
gentamicin,
ceflazidime.
did
not
show
hemolytic
activity.
found
be
able
produce
phytase.
All
findings
obtained
indicate
that
is
promising
as
candidate
has
superior
can
evaluated
future.
further
research
vivo
studies
are
needed
fully
understand
its
mechanism
action
optimize
use
probiotic.
Язык: Английский
Exploring the potential of immobilized phytase to enhance phosphorus uptake by Catharanthus roseus
South African Journal of Botany,
Год журнала:
2023,
Номер
163, С. 715 - 728
Опубликована: Ноя. 22, 2023
Язык: Английский
Taze Kaşar Peynirinden İzole Edilen Lactobacillus brevis'ten Fitaz Enziminin Saflaştırılması ve Biyokimyasal Karakterizasyonu
Türk doğa ve fen dergisi :/Türk doğa ve fen dergisi,
Год журнала:
2024,
Номер
13(1), С. 123 - 127
Опубликована: Март 26, 2024
In
the
last
20
years,
phytase
enzyme
has
attracted
attention
of
scientists
in
fields
environmental
protection,
nutrition
and
biotechnology.
Myo-inositol
hexaphosphate
phosphohydrolase
(phytase),
which
is
a
type
phosphatase
enzyme,
catalyzes
hydrolysis
phytate
into
less
phosphorylated
inorganic
phosphates
phytate.
Phytases
microbial
origin
are
widely
used
biotechnological
applications
(paper
industry,
feed
food
industry
soil
improvement).
present
study,
was
partially
purified
from
Lactobacillus
brevis
NM-34
strain
isolated
fresh
kashar
cheese
pH
temperature
values
at
showed
optimum
activity
were
determined.
L.
243.80
U/mL
as
result
ammonium
sulfate
precipitation.
range
(40-60%),
where
highest
observed,
protein
concentration
measured
0.989
mg/mL.
Km
Vmax
determined
0.0146
mM
1.6
µmol/min,
respectively.
The
found
to
be
5
50
°C,
Based
on
findings
our
research,
this
bacterium
have
unique
properties
that
make
it
suitable
for
use
industrial
applications.
Язык: Английский
Biochemical properties of phytase immobilized and its effect on growth parameters of tomato
Journal of Plant Nutrition and Soil Science,
Год журнала:
2024,
Номер
187(4), С. 533 - 544
Опубликована: Июнь 17, 2024
Abstract
Background
Phosphorus
(P)
is
one
of
the
nonrenewable
resources
critical
importance
in
agricultural
production.
P
present
soil
organic
and
inorganic
forms.
Phytate
constitutes
majority
soil.
binds
strongly
to
solid
phase
becomes
unavailable
for
use
by
plants.
Therefore,
soluble
phytate‐P
ratio
mostly
at
very
low
levels.
Plants
associated
microorganisms
secrete
acids
hydrolyzing
enzymes
such
as
phytase
dissolve
phytate
Both
solubility
activity
are
limiting
properties
uptake
Aims
Our
aim
was
evaluate
effects
immobilized
on
zinc
oxide
nanoparticles
(ZnO
Np)
tomato
plant
(
Solanum
lycopersicum
)
growth
parameters.
In
this
study,
seedling
period
analyzed.
Methods
13
different
bacteria
investigated,
purified
from
Lactobacillus
kefiri
,
showing
highest
activity,
its
biochemical
were
determined.
Phytase
(ZnO)
characterized
X‐ray
diffraction,
Fourier
transform
infrared
spectroscopy,
scanning
electron
microscopes
analysis.
The
ZnONps,
phytase,
free
parameters
investigated.
Tomato
seeds
soaked
with
30
min
room
temperature
sown
pots
containing
suitable
growing
medium.
Vegetative
development
plant,
height,
number
lateral
branches,
main
stem
diameter,
distance
between
nodes,
root,
shoot
length
Results
partially
7.60%
recovery
specific
1758.5
(EU
mg
−1
protein).
Molecular
mass
approx.72
kD,
optimum
pH
values
determined
5.0
70–80°C,
respectively.
Immobilized
caused
a
significant
increase
41.1%
64.1%
36.1%
plants
compared
control.
addition,
observed
side
vegetative
plant.
Conclusions
results
showed
that
enzyme
has
positive
effect
can
be
used
cultivation
future.
Язык: Английский
Impact of a novel caseinolytic protease single mutation on Lactiplantibacillus pentosus growth performance
Process Biochemistry,
Год журнала:
2024,
Номер
145, С. 145 - 152
Опубликована: Июнь 28, 2024
Язык: Английский
Evaluating Enterococcus faecium9 N‐2 as a probiotic candidate from traditional village white cheese
Food Science & Nutrition,
Год журнала:
2023,
Номер
12(3), С. 1847 - 1856
Опубликована: Ноя. 28, 2023
Abstract
In
this
study,
various
functional
and
probiotic
attributes
of
the
Enterococcus
faecium
9
N‐2
strain
isolated
from
village‐style
white
cheese
were
characterized,
while
also
assessing
its
safety.
To
achieve
this,
we
conducted
an
in
vitro
analysis
several
key
properties
exhibited
by
strain.
Notably,
demonstrated
robust
resilience
to
low
pH,
high
bile
salt
concentrations,
lysozyme,
pepsin,
pancreatin,
phenol.
Furthermore,
displayed
exceptional
auto‐aggregation
capabilities
moderate
co‐aggregation
tendencies
when
interacting
with
Escherichia
coli
.
The
cell‐free
supernatant
derived
significant
antimicrobial
activity
against
tested
pathogens.
resistance
gentamicin,
meropenem,
bacitracin,
remaining
susceptible
vancomycin
other
antibiotics.
it
was
found
that
E.
possessed
capacity
produce
phytase
enzyme.
When
all
results
study
are
evaluated,
is
thought
has
superior
properties,
therefore
can
be
used
as
food,
medicine,
animal
feed
future.
addition,
further
vivo
tests
should
performed
fully
understand
effects
mechanisms
action
confirm
safety
effects.
Further
research
clinical
trials
needed
identify
new
strains
potential
properties.
Язык: Английский