Naringin protects against paclitaxel‐induced toxicity in rat testicular tissues by regulating genes in pro‐inflammatory cytokines, oxidative stress, apoptosis, and JNK/MAPK signaling pathways

Journal of Biochemical and Molecular Toxicology, Год журнала: 2024, Номер 38(7)

Опубликована: Июнь 16, 2024

Paclitaxel (PTX), which is actively used in the treatment of many types cancer, has a toxic effect by causing increased oxidative stress testicular tissues. Naringin (NRG) natural flavonoid found plants, and its antioxidant properties are at forefront. This study aims to investigate protective feature NRG PTX-induced toxicity. Thirty-five male Sprague rats were divided into five groups: control, NRG, PTX, PTX + NRG50, NRG100. Rats administered (2 mg/kg, BW) intraperitoneally once daily for first 5 days. Then, between 6th 14th days, (50 100 mg/kg) was orally day. reduced lipid peroxidation tissue capacity (superoxide dismutase, catalase, glutathione peroxidase, glutathione). While reduces mRNA expression levels nuclear factor kappa B, tumor necrosis factor-alpha, interleukin-1 beta, cyclooxygenase-2, interleukin-6, inducible-nitric oxide synthase, mitogen-activated protein kinase 14 (MAPK)14, MAPK15, c-Jun N-terminal kinase, P53, Apaf1, Caspase3, Caspase6, Caspase9, Bax tissues; it caused an increase Nrf2, HO-1, NQO1 Bcl-2 levels. also improved structural functional integrity disrupted PTX. sperm damage alleviated NRG. showed alleviating toxicity increasing stress, inflammation, apoptosis, autophagy.

Язык: Английский

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Pharmacological approaches to enhance mitochondrial biogenesis: focus on PGC-1Α, AMPK, and SIRT1 in cellular health

Molecular Biology Reports, Год журнала: 2025, Номер 52(1)

Опубликована: Фев. 28, 2025

Язык: Английский

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Protective Effects of Syringic Acid Against Oxidative Damage, Apoptosis, Autophagy, Inflammation, Testicular Histopathologic Disorders, and Impaired Sperm Quality in the Testicular Tissue of Rats Induced by Mercuric Chloride

Environmental Toxicology, Год журнала: 2024, Номер 39(10), С. 4803 - 4814

Опубликована: Авг. 3, 2024

Mercury (Hg) is one of the most toxic heavy metals that damage testicular tissue. chloride (HgCl

Язык: Английский

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Exploration of Molecular Mechanisms and Potential Traditional Chinese Medicine Intervention in Acute Liver Injury

Natural Product Communications, Год журнала: 2025, Номер 20(1)

Опубликована: Янв. 1, 2025

Objective This study aimed to explore the potential targets and molecular mechanisms underlying progression of acute liver injury, as well predict traditional Chinese medicines associated with key targets. Methods Differentially expressed genes (DEGs) injury were first screened. Then immune infiltration analysis was performed using CIBERSORT algorithm, followed by Weighted Gene Co-Expression Network Analysis (WGCNA) analysis. Subsequently, Ontology (GO) Kyoto Encyclopedia Genomes (KEGG) analyses overlapping between DEG WGCNA DAVID database. The main active components predicted determined through network pharmacology In vitro experiment observe effects different concentrations quercetin on inflammatory response LPS-induced J774A.1 macrophages. Results results showed elevated M1 macrophages activated dendritic cells in injury. A total 40 DEGs obtained, which significantly enriched four pathways, including Toll-like receptor signaling pathway ( P < .05). Furthermore, eight identified, pharmacological revealed their ingredient. experimental that reduced mRNA levels IL-6, TNF-α, IL-1β, NF-κB protein IL-6 TNF-α Additionally, inhibited release NO Conclusion present provides further evidence potentially exert pro-inflammatory via toll-like pathways. Notably, demonstrates inhibitory properties this process, its mechanism action may be intricately linked TLR4-NF-κB pathway.

Язык: Английский

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Biological and Molecular Efficiency of Paracentrotus lividus Shell in vitro Study: Antioxidant and Angiogenesis Effects Against T47D Breast Cancer Cell Line Via Nrf2/HMOX-1/ and HIF-1α /VEGF Signaling Pathways

Cell Biochemistry and Biophysics, Год журнала: 2025, Номер unknown

Опубликована: Фев. 4, 2025

Язык: Английский

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Hesperidin counteracts chlorpyrifos-induced neurotoxicity by regulating oxidative stress, inflammation, and apoptosis in rats

Metabolic Brain Disease, Год журнала: 2023, Номер 39(4), С. 509 - 522

Опубликована: Дек. 18, 2023

Язык: Английский

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Polyphenols Modulate the miRNAs Expression that Involved in Glioblastoma

Mini-Reviews in Medicinal Chemistry, Год журнала: 2024, Номер 24(21), С. 1953 - 1969

Опубликована: Апрель 19, 2024

: Glioblastoma multiforme (GBM), a solid tumor that develops from astrocytes, is one of the most aggressive types brain cancer. While there have been improvements in efficacy treating GBM, many problems remain, especially with traditional therapy methods. Therefore, recent studies extensively focused on developing novel therapeutic agents for combating glioblastoma. Natural polyphenols studied their potential as chemopreventive and chemotherapeutic due to wide range positive qualities, including antioxidant, antiinflammatory, cytotoxic, antineoplastic, immunomodulatory activities. These natural compounds suggested act via modulated various macromolecules within cells, microRNAs (miRNAs), which play crucial role molecular milieu. In this article, we focus how may inhibit growth by influencing expression key miRNAs regulate oncogenes suppressor genes

Язык: Английский

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Bioinformatic analyses reveal lysosomal-associated protein transmembrane 5 as a potential therapeutic target in lipotoxicity-induced injury in diabetic kidney disease

Renal Failure, Год журнала: 2024, Номер 46(2)

Опубликована: Июнь 4, 2024

Emerging data have revealed that damage to tubular epithelial cell is a driving force in the progression of diabetic kidney disease (DKD). However, specific mechanisms by which lipotoxicity contributes injury these cells, thereby influencing development DKD, are yet be fully understood. Here, we analyzed GSE 30529 microarray datasets human tubulointerstitial tissue samples from Gene Expression Omnibus database (GEO). Concurrently, conducted RNA-sequencing on palmitic acid (PA)-treated renal proximal tubule cells (HK2 cells). After normalization, differentially expressed genes (DEGs) were screened R software and gene ontology (GO) enrichment analysis was conducted, lysosomal-associated protein transmembrane 5 (LAPTM5) finally selected. Our findings indicate expression LAPTM5 obviously increased DKD patients, correlation between LAPTM5, other clinical parameters using Spearman analysis. The potential as prognostic biomarker for further consolidated through receiver operating characteristic (ROC) To verify function established mouse or vitro systems mimicking DKD. results showed consistent upregulation also found linked with inflammatory mediators within context Additionally, silencing significantly downregulated mRNA factors PA-treated HK2 cells. These therapeutic treatment target This discovery paves way future research targeted interventions aimed at mitigating this prevalent condition.

Язык: Английский

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Effect of Mesembryanthemum crystallinum and its derived D‐pinitol on HMG‐CoA reductase and tyloxapol‐induced hyperlipedemia

eFood, Год журнала: 2024, Номер 5(6)

Опубликована: Ноя. 8, 2024

Abstract Several previous research indicate that treating Mesembryanthemum crystallinum may aid in reducing adipogenesis and triacylglycerol level improving hyperglycemia diabetes. Therefore, the present study was designed to evaluate effectiveness of M. extract (MCE) combating obesity lowering fat/lipid/cholesterol levels. The aimed investigate molecular docking model targeting 3‐hydroxy‐3‐methylglutaryl‐CoA reductase (HMGCR) using MCE‐derived d ‐pinitol or atorvastatin, an inhibitor HMGCR. In this study, histological alterations liver tyloxapol‐induced hyperlipidemia (TIH) model, hyperlipidemia‐related markers serum, HMGCR activity, cell viability HepG2 cells were analyzed. Our findings revealed tyloxapol treatment significantly led increased indicators cardiovascular‐associated risk indices. However, MCE effectively mitigated hepatic fat accumulation increase cholesterol This achieved through improvements metabolic parameters, ultimately ameliorating TIH. These beneficial results suggest be a strong potential for diseases.

Язык: Английский

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Histopathological and Antioxidant Effects of Bromelain on Kidney Tissue of Tyloxapol-Induced Hyperlipidemic Rats

Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Год журнала: 2024, Номер 29(2), С. 413 - 422

Опубликована: Май 25, 2024

This study was designed to investigate the nephroprotective effect of Bromelain against oxidative stress induced by Tyloxopol-induced hyperlipidemia in rats. Rats (n=6) were divided into three groups. I: Control (C) Group: No treatment given and only distilled water given; II: Tyloxapol (TX) Group receiving (400 mg/kg, i.p); III: + (TX+BR) i.p.) group (250 o.d.) for 18 days. As a result administration, parameters SOD CAT levels decreased, while MDA increased. In addition, histopathologic variations detected kidney sections. On other hand, lipid peroxidation decreased most studied improved with combined administration (TX+BR group). According results obtained, bromelain may be potent alternative agent protective further studies.

Язык: Английский

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