Organoids with a Type 1 Collagen Scaffold to Model Bacterial Cancer Therapy DOI Creative Commons
Lydia Farrell, Cleo Bonnet, Alethea Tang

и другие.

Cells, Год журнала: 2025, Номер 14(7), С. 524 - 524

Опубликована: Апрель 1, 2025

Bacterial cancer therapy (BCT) is emerging as an important option for the treatment of solid tumours, with promising outcomes in preclinical trials. Further progress hampered by incomplete understanding how oncotropic bacteria, such attenuated strains Salmonella enterica serovar Typhimurium, colonise tumours and responses both bacteria tumour cells to this colonisation. To model this, we developed organoids that are permissive bacterial colonisation, replacing conventional commercially available extracellular matrix (e.g., Matrigel) a type I collagen scaffold. A comparison two matrices indicated 1 permitted initial infection efficiency more than 5-times greater Matrigel. In addition, subsequent growth within expanded cell numbers over 10-fold 4 days infection. These allow visualisation chemoattraction, invasion population interior lumen, will permit future optimisation BCT. establishing patient-derived organoids, demonstrate platform developing personalised treatments exploiting

Язык: Английский

Organoids with a Type 1 Collagen Scaffold to Model Bacterial Cancer Therapy DOI Creative Commons
Lydia Farrell, Cleo Bonnet, Alethea Tang

и другие.

Cells, Год журнала: 2025, Номер 14(7), С. 524 - 524

Опубликована: Апрель 1, 2025

Bacterial cancer therapy (BCT) is emerging as an important option for the treatment of solid tumours, with promising outcomes in preclinical trials. Further progress hampered by incomplete understanding how oncotropic bacteria, such attenuated strains Salmonella enterica serovar Typhimurium, colonise tumours and responses both bacteria tumour cells to this colonisation. To model this, we developed organoids that are permissive bacterial colonisation, replacing conventional commercially available extracellular matrix (e.g., Matrigel) a type I collagen scaffold. A comparison two matrices indicated 1 permitted initial infection efficiency more than 5-times greater Matrigel. In addition, subsequent growth within expanded cell numbers over 10-fold 4 days infection. These allow visualisation chemoattraction, invasion population interior lumen, will permit future optimisation BCT. establishing patient-derived organoids, demonstrate platform developing personalised treatments exploiting

Язык: Английский

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