GTP-tubulin
is
preferentially
incorporated
at
growing
microtubule
ends,
but
the
biochemical
mechanism
by
which
bound
nucleotide
regulates
strength
of
tubulin:tubulin
interactions
debated.
The
'self-acting'
(cis)
model
posits
that
(GTP
or
GDP)
to
a
particular
tubulin
dictates
how
strongly
interacts,
whereas
'interface-acting'
(trans)
interface
two
dimers
determinant.
We
identified
testable
difference
between
these
mechanisms
using
mixed
simulations
elongation:
with
self-acting
nucleotide,
plus-
and
minus-end
growth
rates
decreased
in
same
proportion
amount
GDP-tubulin,
interface-acting
plus-end
disproportionately.
then
experimentally
measured
elongation
nucleotides
observed
disproportionate
effect
GDP-tubulin
on
rates.
Simulations
were
consistent
binding
'poisoning'
plus-ends
not
minus-ends.
Quantitative
agreement
experiments
required
exchange
terminal
subunits
mitigate
poisoning
there.
Our
results
indicate
interfacial
determines
interaction
strength,
thereby
settling
longstanding
debate
over
state
dynamics.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Май 4, 2023
Abstract
GTP-tubulin
is
preferentially
incorporated
at
growing
microtubule
ends,
but
the
biochemical
mechanism
by
which
bound
nucleotide
regulates
strength
of
tubulin:tubulin
interactions
debated.
The
‘self-acting’
(cis)
model
posits
that
(GTP
or
GDP)
to
a
particular
tubulin
dictates
how
strongly
interacts,
whereas
‘interface-acting’
(trans)
interface
two
dimers
determinant.
We
identified
testable
difference
between
these
mechanisms
using
mixed
simulations
elongation:
with
self-acting
nucleotide,
plus-
and
minus-end
growth
rates
decreased
in
same
proportion
amount
GDP-tubulin,
interface-acting
plus-end
disproportionately.
then
experimentally
measured
elongation
nucleotides
observed
disproportionate
effect
GDP-tubulin
on
rates.
Simulations
were
consistent
binding
‘poisoning’
plus-ends
not
minus-ends.
Quantitative
agreement
experiments
required
exchange
terminal
subunits
mitigate
poisoning
there.
Our
results
indicate
interfacial
determines
interaction
strength,
thereby
settling
longstanding
debate
over
state
dynamics.
Abstract
GTP-tubulin
is
preferentially
incorporated
at
growing
microtubule
ends,
but
the
biochemical
mechanism
by
which
bound
nucleotide
regulates
strength
of
tubulin:tubulin
interactions
debated.
The
‘self-acting’
(cis)
model
posits
that
(GTP
or
GDP)
to
a
particular
tubulin
dictates
how
strongly
interacts,
whereas
‘interface-acting’
(trans)
interface
two
dimers
determinant.
We
identified
testable
difference
between
these
mechanisms
using
mixed
simulations
elongation:
with
self-acting
plus-
and
minus-end
growth
rates
decreased
in
same
proportion
amount
GDP-tubulin,
interface-acting
plus-end
disproportionately.
then
experimentally
measured
elongation
nucleotides
observed
disproportionate
effect
GDP-tubulin
on
rates.
Simulations
were
consistent
binding
‘poisoning’
plus-ends
not
minus-ends.
Quantitative
agreement
experiments
required
exchange
terminal
subunits
mitigate
poisoning
there.
Our
results
indicate
interfacial
determines
interaction
strength,
thereby
settling
longstanding
debate
over
state
dynamics.
GTP-tubulin
is
preferentially
incorporated
at
growing
microtubule
ends,
but
the
biochemical
mechanism
by
which
bound
nucleotide
regulates
strength
of
tubulin:tubulin
interactions
debated.
The
‘self-acting’
(cis)
model
posits
that
(GTP
or
GDP)
to
a
particular
tubulin
dictates
how
strongly
interacts,
whereas
‘interface-acting’
(trans)
interface
two
dimers
determinant.
We
identified
testable
difference
between
these
mechanisms
using
mixed
simulations
elongation:
with
self-acting
nucleotide,
plus-
and
minus-end
growth
rates
decreased
in
same
proportion
amount
GDP-tubulin,
interface-acting
plus-end
disproportionately.
then
experimentally
measured
elongation
nucleotides
observed
disproportionate
effect
GDP-tubulin
on
rates.
Simulations
were
consistent
binding
‘poisoning’
plus-ends
not
minus-ends.
Quantitative
agreement
experiments
required
exchange
terminal
subunits
mitigate
poisoning
there.
Our
results
indicate
interfacial
determines
interaction
strength,
thereby
settling
longstanding
debate
over
state
dynamics.
GTP-tubulin
is
preferentially
incorporated
at
growing
microtubule
ends,
but
the
biochemical
mechanism
by
which
bound
nucleotide
regulates
strength
of
tubulin:tubulin
interactions
debated.
The
'self-acting'
(cis)
model
posits
that
(GTP
or
GDP)
to
a
particular
tubulin
dictates
how
strongly
interacts,
whereas
'interface-acting'
(trans)
interface
two
dimers
determinant.
We
identified
testable
difference
between
these
mechanisms
using
mixed
simulations
elongation:
with
self-acting
nucleotide,
plus-
and
minus-end
growth
rates
decreased
in
same
proportion
amount
GDP-tubulin,
interface-acting
plus-end
disproportionately.
then
experimentally
measured
elongation
nucleotides
observed
disproportionate
effect
GDP-tubulin
on
rates.
Simulations
were
consistent
binding
'poisoning'
plus-ends
not
minus-ends.
Quantitative
agreement
experiments
required
exchange
terminal
subunits
mitigate
poisoning
there.
Our
results
indicate
interfacial
determines
interaction
strength,
thereby
settling
longstanding
debate
over
state
dynamics.
GTP-tubulin
is
preferentially
incorporated
at
growing
microtubule
ends,
but
the
biochemical
mechanism
by
which
bound
nucleotide
regulates
strength
of
tubulin:tubulin
interactions
debated.
The
‘self-acting’
(cis)
model
posits
that
(GTP
or
GDP)
to
a
particular
tubulin
dictates
how
strongly
interacts,
whereas
‘interface-acting’
(trans)
interface
two
dimers
determinant.
We
identified
testable
difference
between
these
mechanisms
using
mixed
simulations
elongation:
with
self-acting
nucleotide,
plus-
and
minus-end
growth
rates
decreased
in
same
proportion
amount
GDP-tubulin,
interface-acting
plus-end
disproportionately.
then
experimentally
measured
elongation
nucleotides
observed
disproportionate
effect
GDP-tubulin
on
rates.
Simulations
were
consistent
binding
‘poisoning’
plus-ends
not
minus-ends.
Quantitative
agreement
experiments
required
exchange
terminal
subunits
mitigate
poisoning
there.
Our
results
indicate
interfacial
determines
interaction
strength,
thereby
settling
longstanding
debate
over
state
dynamics.
GTP-tubulin
is
preferentially
incorporated
at
growing
microtubule
ends,
but
the
biochemical
mechanism
by
which
bound
nucleotide
regulates
strength
of
tubulin:tubulin
interactions
debated.
The
'self-acting'
(cis)
model
posits
that
(GTP
or
GDP)
to
a
particular
tubulin
dictates
how
strongly
interacts,
whereas
'interface-acting'
(trans)
interface
two
dimers
determinant.
We
identified
testable
difference
between
these
mechanisms
using
mixed
simulations
elongation:
with
self-acting
nucleotide,
plus-
and
minus-end
growth
rates
decreased
in
same
proportion
amount
GDP-tubulin,
interface-acting
plus-end
disproportionately.
then
experimentally
measured
elongation
nucleotides
observed
disproportionate
effect
GDP-tubulin
on
rates.
Simulations
were
consistent
binding
'poisoning'
plus-ends
not
minus-ends.
Quantitative
agreement
experiments
required
exchange
terminal
subunits
mitigate
poisoning
there.
Our
results
indicate
interfacial
determines
interaction
strength,
thereby
settling
longstanding
debate
over
state
dynamics.