Cataract induction in an arthropod reveals how lens crystallins contribute to the formation of biological glass DOI Creative Commons
Amartya T. Mitra, Shubham Rathore,

Augusta Jester

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 14, 2024

Summary Lenses are vital components of eyes, crafted through the precise arrangement proteins to achieve transparency and focusing power, placed appropriately within eye for retina receive sharp, focused images. Maintaining these states is challenging due substantial post-embryonic lens growth. Here, we gain insights into required processes by exploring consequences silencing Lens3, a key protein, during growth in Thermonectus marmoratus sunburst diving beetle larvae. We show that lens3RNAi leads opaque aggregates reminiscent vertebrate ‘cataracts,’ causing projection degraded images which impact behaviour low light. Our findings uncover how complex biological lenses constructed further support visual input inconsequential coordinated this clade. highlight utility insect as tractable model system investigate aetiology cataracts refractive errors sophisticated camera-type eye. Highlights ● Crystallin protein knockdown induces central an Cataractous project blurry but have unchanged focal length Resulting image degradation impacts light conditions Contrary vertebrates, vision does not affect state

Язык: Английский

Heterochrony in orthodenticle expression is associated with ommatidial size variation between Drosophila species DOI Creative Commons
Montserrat Torres-Oliva, Elisa Buchberger, Alexandra D. Buffry

и другие.

BMC Biology, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 4, 2025

Abstract Background The compound eyes of insects exhibit extensive variation in ommatidia number and size, which affects how they see underlies adaptations their vision to different environments lifestyles. However, very little is known about the genetic developmental bases differences eye size. We previously showed that larger Drosophila mauritiana compared D. simulans are generally caused by size rather than number. Furthermore, we identified an X-linked chromosomal region results when introgressed into . Results Here, used a combination fine-scale mapping gene expression analysis further investigate positional candidate genes on X chromosome. found earlier orthodenticle (otd) during ommatidial maturation , show this required for correct organisation melanogaster discovered activity otd enhancer consistent with difference between species, sequence driving When driven prematurely development, grow larger, supporting possible role timing regulating also potential direct targets Otd differentially expressed maturation. Conclusions Taken together, our suggest differential may contribute natural provides new insights mechanisms underlying regulation evolution insects.

Язык: Английский

Процитировано

0
Ultrastructural and light/dark adaptational characteristics of the compound eyes in the fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae) DOI
Han Ying,

Qing‐Xiao Chen

Arthropod Structure & Development, Год журнала: 2025, Номер 86, С. 101449 - 101449

Опубликована: Апрель 10, 2025

Язык: Английский

Процитировано

0
Heterochrony inorthodenticleexpression is associated with ommatidial size variation betweenDrosophilaspecies DOI Creative Commons
Montserrat Torres-Oliva, Elisa Buchberger, Alexandra D. Buffry

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2021, Номер unknown

Опубликована: Март 17, 2021

Abstract The compound eyes of insects exhibit extensive variation in ommatidia number and size, which affects how they see underlies adaptations their vision to different environments lifestyles. However, very little is known about the genetic developmental bases differences eye size. We previously showed that larger Drosophila mauritiana compared D. simulans generally caused by size rather than number. Furthermore, we identified an X-linked chromosomal region results when introgressed into . Here, used a combination fine-scale mapping gene expression analysis further investigate positional candidate genes on X chromosome. found earlier orthodenticle (otd) during ommatidial maturation third instar larvae , show this required for correct organisation melanogaster discovered activity otd enhancer consistent with difference between species, sequence driving also potential direct targets Otd are differentially expressed maturation. Taken together, our suggest differential timing contributes natural simulans, provides new insights mechanisms underlying regulation evolution insects.

Язык: Английский

Процитировано

4
Cataract induction in an arthropod reveals how lens crystallins contribute to the formation of biological glass DOI Creative Commons
Amartya T. Mitra, Shubham Rathore,

Augusta Jester

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 14, 2024

Summary Lenses are vital components of eyes, crafted through the precise arrangement proteins to achieve transparency and focusing power, placed appropriately within eye for retina receive sharp, focused images. Maintaining these states is challenging due substantial post-embryonic lens growth. Here, we gain insights into required processes by exploring consequences silencing Lens3, a key protein, during growth in Thermonectus marmoratus sunburst diving beetle larvae. We show that lens3RNAi leads opaque aggregates reminiscent vertebrate ‘cataracts,’ causing projection degraded images which impact behaviour low light. Our findings uncover how complex biological lenses constructed further support visual input inconsequential coordinated this clade. highlight utility insect as tractable model system investigate aetiology cataracts refractive errors sophisticated camera-type eye. Highlights ● Crystallin protein knockdown induces central an Cataractous project blurry but have unchanged focal length Resulting image degradation impacts light conditions Contrary vertebrates, vision does not affect state

Язык: Английский

Процитировано

0