Conformational dynamics in specialized C₂H₂ zinc finger domains enable zinc‐responsive gene repression in S. pombe

Protein Science, Год журнала: 2025, Номер 34(2)

Опубликована: Янв. 25, 2025

Abstract Loz1 is a zinc‐responsive transcription factor in fission yeast that maintains cellular zinc homeostasis by repressing the expression of genes required for uptake high conditions. Previous deletion analysis found region containing two tandem C 2 H zinc‐fingers and an upstream “accessory domain” rich histidine, lysine, arginine residues to be sufficient zinc‐dependent DNA binding gene repression. Here we report unexpected biophysical properties this pair seemingly classical fingers. Isothermal titration calorimetry NMR spectroscopy reveal distinct events localized spectra complex dynamic behavior spanning time scales from fast 10 −12 –10 −10 slow >10 0 s. Slow exchange due cis‐trans isomerization TGERP linker results doubling many signals protein. Conformational on −3 s timescale throughout first finger distinguishes it second linked weaker affinity zinc. These findings mechanism sensing illuminate how protein's rough free‐energy landscape enables sensing, regulated expression.

Язык: Английский

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Genome-wide analysis of the C2H2-type zinc finger protein family in rice (Oryza sativa) and the role of OsC2H2.35 in cold stress response

Plant Stress, Год журнала: 2025, Номер 15, С. 100772 - 100772

Опубликована: Фев. 15, 2025

Язык: Английский

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The Arthropoda-specific Tramtrack group BTB protein domains use previously unknown interface to form hexamers

eLife, Год журнала: 2024, Номер 13

Опубликована: Сен. 2, 2024

BTB (bric-a-brack, Tramtrack, and broad complex) is a diverse group of protein-protein interaction domains found within metazoan proteins. Transcription factors contain dimerizing subtype with characteristic N-terminal extension. The Tramtrack (TTK) distinct type domain, which can multimerize. Single-particle cryo-EM microscopy revealed that the TTK-type assemble into hexameric structure consisting three canonical dimers connected through previously uncharacterized interface. We demonstrated are only in Arthropods have undergone lineage-specific expansion modern insects. Drosophila genome encodes 24 transcription domains, whereas four non-TTK-type domains. Yeast two-hybrid analysis an unusually potential for heteromeric associations presumably dimer-dimer Thus, structurally functionally protein specific to Arthropodan factors.

Язык: Английский

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Conformational dynamics in specialized C₂H₂zinc finger domains enable zinc-responsive gene repression inS. pombe

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 21, 2024

Abstract Loz1 is a zinc-responsive transcription factor in fission yeast that maintains cellular zinc homeostasis by repressing the expression of genes required for uptake high conditions. Previous deletion analysis found region containing two tandem C 2 H zinc-fingers and an upstream “accessory domain” rich histidine, lysine, arginine residues to be sufficient zinc-dependent DNA binding gene repression. Here we report unexpected biophysical properties this pair seemingly classical fingers. Isothermal titration calorimetry NMR spectroscopy reveal distinct events localized spectra complex dynamic behavior responsive spanning time scales from fast 10 −12 -10 −10 slow > 0 sec. Slow exchange due cis-trans isomerization TGERP linker results doubling many signals protein. Conformational on −3 s timescale throughout first finger distinguishes it second linked weaker affinity zinc. These findings mechanism sensing illuminate how protein’s rough free-energy landscape enable sensing, regulated expression.

Язык: Английский

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Genome-Wide Analysis of C2H2 Zinc Finger Gene Family and Exploration of Genes Related to Prickle Development in Zanthoxylum armatum

Horticulturae, Год журнала: 2024, Номер 10(12), С. 1371 - 1371

Опубликована: Дек. 20, 2024

Zanthoxylum armatum (Z. armatum) is a significant economic tree species known for its medicinal and edible properties. However, the presence of prickles on Z. poses considerable challenge to advancement industry. Numerous studies have indicated that C2H2 zinc finger protein (C2H2-ZFPs) families are crucial in development plant trichomes or prickles. This study identified 78 ZaC2H2 genes from genome, categorizing them into three groups analyzing their physicochemical properties, chromosomal locations, conserved domains, gene structures. The evolutionary analysis indicates amplification primarily results whole-genome duplication segmental duplication, these undergone strong purifying selection pressure throughout history. cis-acting elements revealed they contain various hormone response elements, such as ABRE, AuxRR, CGTCA motif, GARE motifs, TCA which responsive ABA, IAA, MeJA, GA, SA signals. RT-qPCR was employed assess expression levels candidate ZaC2H2-45, ZaC2H2-46, ZaC2H2-49, ZaC2H2-55 under treatment five hormones. ZaC2H2-46 were significantly up-regulated NAA, SA, MeJA treatments. These will help further understand characteristics family provide theoretical basis studying

Язык: Английский

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Genome-Wide Analysis of the C2h2-Type Zinc Finger Protein Family in Rice (Oryza Sativa) and the Role of Osc2h2.35 in Cold Stress Response

Опубликована: Янв. 1, 2024

Язык: Английский

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