The role of bacterial metabolism in antimicrobial resistance

Nature Reviews Microbiology, Год журнала: 2025, Номер unknown

Опубликована: Фев. 20, 2025

Язык: Английский

Metabolic remodeling of microorganisms by obligate intracellular parasites alters mutualistic community composition

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 2, 2025

ABSTRACT Bacteria carry many types of obligate intracellular parasites, including plasmids and bacteriophage. During infection, these parasites redirect resources away from bacterial processes toward parasite production. Because parasite-induced metabolic changes influence host traits such as growth rate, nutrient uptake, waste excretion, parasitic infection should alter how microbes contribute to important community ecosystem functions. Yet there are few empirical tests shapes metabolically-mediated interactions between non-host species. Here, we integrated a genome-scale modeling approach with an in vitro cross-feeding system investigate the consequences two Escherichia coli : conjugative plasmid F128 filamentous phage M13. We examined impact on bacteria multispecies composed E. , Salmonella enterica, Methylobacterium extorquens . Modeling predicted that have for rate secretion reuptake carbon byproducts. These theoretical results aligned broadly experiments, where found changed excretion profile inducing net externalization lactate. also parasite-driven metabolism increased density species composition were generalizable across some genotypes. Our work emphasizes infected by different metabolisms than uninfected cells demonstrates shifts can significant microbial structure function. IMPORTANCE The shape function communities variety ways, killing their hosts or transferring genetic material. This study uses combination flux balance analysis consisting enterica M13, change contributions via remodeling. Flux suggests demand processes, leading identities concentrations compounds externalize into environment. finding is supported experimental results, which additionally show induce production findings extend our understanding bacteriophage communities.

Язык: Английский

An industrial available platform for high-yield, plasmid-free recombinant protein production in E. coli based on advanced cSAT scheme

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 142208 - 142208

Опубликована: Март 1, 2025

Язык: Английский