The
coronavirus-induced
disease
19
(COVID-19)
pandemic,
caused
by
severe
acute
respiratory
syndrome
coronavirus-2
(SARS-CoV-2)
infections,
has
had
a
devastating
impact
on
millions
of
lives
globally,
with
mortality
rates
and
catastrophic
social
implications.
Developing
tools
for
effective
vaccine
strategies
platforms
is
essential
controlling
preventing
the
recurrence
such
pandemics.
Moreover,
molecular
virology
that
facilitate
study
viral
pathogens,
mutations,
interactions
various
host
proteins
are
essential.
Viral
replicon-
virus-like
particle
(VLP)-based
systems
excellent
examples
tools.
This
review
outlines
importance,
advantages,
disadvantages
both
VLP-based
have
been
developed
SARS-CoV-2
helped
scientific
community
in
dimming
intensity
COVID-19
pandemic.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 14, 2025
Raman
spectroscopy
is
an
analytical
method
capable
of
detecting
various
microorganisms
and
small
particles.
Here,
we
used
25-1000
nm
polystyrene
particles
in
aqueous
suspensions,
which
are
comparable
size
to
viral
aggregates,
determine
the
limit
detection
(LOD)
a
confocal
microscope.
We
collected
spectra
using
785
wavelength
laser
with
power
300
mW
10
s
exposure
time
5×
objective
lens.
detected
most
prominent
peak
at
1001
cm-1,
corresponding
ring
breathing
mode.
established
minimum
maximum
LOD
(LODmin
LODmax)
Kernel
partial
least-squares
model.
The
smallest
50
was
identified
as
1.80
×
1012-8.31
1012
particle/mL,
for
largest
1000
nm,
5.11
108-2.53
109
particle/mL.
demonstrated
that
nondestructive
under
these
conditions
by
comparing
particle
before
after
collecting
dynamic
light
scattering.
Due
their
similarity
this
systematic
characterization
provides
detailed
information
on
spectral
signatures
suspensions.
These
findings
establish
foundation
suspensions
highlight
its
potential
tool
real-time
monitoring
vaccine
manufacturing.
