Quantification of Pectolinarin in Rat Plasma Using UPLC‐MS/MS and Its Pharmacokinetic Analysis DOI Open Access

Mengmeng Shao,

Runrun Wang,

Congcong Wen

и другие.

Biomedical Chromatography, Год журнала: 2025, Номер 39(4)

Опубликована: Фев. 19, 2025

ABSTRACT Pectolinarin is a flavonoid compound known for its wound‐healing properties, including anti‐inflammatory and antibacterial effects. In this study, we employed UPLC‐MS/MS to quantify pectolinarin in rat plasma investigate pharmacokinetics. Plasma samples were processed using an acetonitrile precipitation method. Chromatographic separation was performed on UPLC BEH column with gradient mobile phase of acetonitrile‐water (containing 0.1% formic acid). Detection carried out electrospray ionization (ESI) tandem mass spectrometry multiple reaction monitoring (MRM) mode positive ionization, targeting transitions m / z 623.3 → 315.3 370.5 125.0 the IS. The results demonstrated that exhibited acceptable linearity within concentration range 1.2 2300 ng/mL ( r > 0.995). intraday interday precision, expressed as relative standard deviation (RSD), below 9.2%. Accuracy ranged from 97.3% 108.3%, average recovery exceeding 94.7%. matrix effect between 97.8% 105.3%. method successfully applied evaluate pharmacokinetics rats following both oral intravenous administration. absolute bioavailability determined be 0.28%.

Язык: Английский

Quantification of Pectolinarin in Rat Plasma Using UPLC‐MS/MS and Its Pharmacokinetic Analysis DOI Open Access

Mengmeng Shao,

Runrun Wang,

Congcong Wen

и другие.

Biomedical Chromatography, Год журнала: 2025, Номер 39(4)

Опубликована: Фев. 19, 2025

ABSTRACT Pectolinarin is a flavonoid compound known for its wound‐healing properties, including anti‐inflammatory and antibacterial effects. In this study, we employed UPLC‐MS/MS to quantify pectolinarin in rat plasma investigate pharmacokinetics. Plasma samples were processed using an acetonitrile precipitation method. Chromatographic separation was performed on UPLC BEH column with gradient mobile phase of acetonitrile‐water (containing 0.1% formic acid). Detection carried out electrospray ionization (ESI) tandem mass spectrometry multiple reaction monitoring (MRM) mode positive ionization, targeting transitions m / z 623.3 → 315.3 370.5 125.0 the IS. The results demonstrated that exhibited acceptable linearity within concentration range 1.2 2300 ng/mL ( r > 0.995). intraday interday precision, expressed as relative standard deviation (RSD), below 9.2%. Accuracy ranged from 97.3% 108.3%, average recovery exceeding 94.7%. matrix effect between 97.8% 105.3%. method successfully applied evaluate pharmacokinetics rats following both oral intravenous administration. absolute bioavailability determined be 0.28%.

Язык: Английский

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