
Plants, Год журнала: 2025, Номер 14(8), С. 1236 - 1236
Опубликована: Апрель 18, 2025
Drought stress is one of the key environmental factors restricting germination alfalfa seeds (Medicago sativa L.). Nanopriming an innovative seed-priming technology able to meet economic, agronomic, and needs in agriculture. However, use conventional nanomaterials hampered by high costs, risks, biotoxicity. In this study, we synthesized iron oxide nanoparticles (Fe3O4NPs) using seasonal Ginkgo biloba leaf extracts (collected from August November) obtained via enzymatic ultrasonic-assisted method. The Fe3O4NPs were characterized SEM, EDS, DLS, FTIR, UV-Vis, XRD. To investigate effects Fe3O4NP priming on seed under drought stress, pot experiments conducted with five concentrations (unprimed, 0, 20, 40, 60 mg/L) three PEG-6000 (0%, 10%, 15%) simulate normal, moderate, severe conditions. results showed that collected November exhibited highest flavonoid content (12.8 mg/g), successfully yielding bioactive-capped spherical a particle size 369.5 ± 100.6 nm. Germination revealed (15% PEG-6000), 40 mg/L treatment most effectively enhanced vigor, increasing rate, vigor index, α-amylase activity 22.1%, 189.4%, 35.5% (p < 0.05), respectively, compared controls. Under moderate (10% 20 optimally improved traits, rate 25.5% seedling elongation 115.6%. demonstrated morphological adaptations seedlings: significantly increased lateral root numbers, while surface area 20.5% preserved roots’ structural integrity These findings highlight ultrasonic methods exhibit promising agricultural potential. optimal resistance modulating morphology, concentration-specific efficacy varying intensities.
Язык: Английский