Cancer-associated fibroblasts derived-exosomal circ_0076535 promotes esophageal squamous cell carcinoma progression DOI

Ningning Kang,

Wei Ge,

Jinxiu Hu

и другие.

Technology and Health Care, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Background Esophageal cancer (EC) is a common malignant tumor of the digestive tract and an important health-related problem in many developing countries. squamous cell carcinoma (ESCC) most subtype EC. The cancer-associated fibroblasts (CAFs) are major stromal cells ESCC microenvironment. They play role proliferation, metastasis, angiogenesis chemotherapy resistance through paracrine processes. However, roles circRNAs enriched CAF-derived exosmes have not been reported. Objective To explore mechanisms how CAF affects proliferation metastasis processes to investigate exosomes. Methods Exosomes were isolated from conditional medium using differential ultracentrifugation, then validated by Nanosight analysis. Exosome secretion inhibitor-GW4869 validates pro-carcinogenic qRT-PCR showed highest expression circ_0076535 exosomal CircRNA, knockdown it confirmed its function. Online bioinformatics tool was utilized predict potential target gene circ_0076535, captured miR-145-5p as with high predictive value. targeting association between further dual luciferase reporter experiment. stimulation tumour development EMT exosome vivo. Results In our research, we found that exosomes increased migration, invasion cells. Circ_0076535 highly CAF-exosomes transferred into directly depend on internalization CAF-exosomal level induced EMT. Mechanistic experiments revealed acted sponge absorb activated NF-κB signaling pathway. Conclusions Conclusively, promoted progression via miR-145-5p/NF-κB axis expected be biomarker for early diagnosis treatment ESCC.

Язык: Английский

Neoadjuvant Chemoradiotherapy vs Chemoimmunotherapy for Esophageal Squamous Cell Carcinoma DOI Creative Commons
Xufeng Guo, Chunji Chen, Jinbo Zhao

и другие.

JAMA Surgery, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

Importance The association of neoadjuvant chemoimmunotherapy (NCIT) vs chemoradiotherapy (NCRT) with tumor downstaging and survival in locally advanced esophageal squamous cell carcinoma (ESCC) remains unclear because limited evidence. Objective To compare the associations NCIT NCRT regression long-term patients ESCC. Design, Setting, Participants In this comparative effectiveness research study, from January 2016 to March 2023, ESCC who underwent esophagectomy following or were identified a prospective database 8 high-volume surgery centers China. Follow-up began on date continued until last recorded contact 2024, whichever occurred first. Data analyzed between April September 2024. Main Outcomes Measures primary end points 2-year overall (OS) disease-free (DFS). Secondary included major pathologic response (MPR) complete (pCR). Cox proportional hazard analysis was used investigate risk factors for OS DFS. Results study 1428 (median [IQR] age, 63 [57-68] years; 1184 men [82.9%]), 704 group 724 group. After propensity score matching, there 532 each (81.3% 71.3%; ratio, 1.57; 95% CI, 1.26-1.96; P < .001) DFS (73.9% 63.4%; 1.37; 1.11-1.69; rates significantly higher than had MPR rate that (71.8% 61.5%), whereas pCR similar (25.9% 22.9%). Multivariable demonstrated independently associated both exhibited lower recurrence (126 [23.7%] 190 [35.7%]) distant metastasis (72 [13.5%] 133 [25.0%]), although locoregional (98 [18.4%] 111 [20.9%]). Better obtained group, regardless whether adjuvant immunotherapy given. Conclusions Relevance Compared NCRT, receiving better decrease may be main reason, but further randomized clinical trials are needed verify finding.

Язык: Английский

Процитировано

2

Survival benefits of postoperative radiotherapy in esophageal cancer during the immunotherapy era:a retrospective cohort study based on the SEER database and a single-center registry in China DOI Creative Commons
Qian Zhang, Jia‐Tao Zhang, Jiaqi Gu

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Фев. 24, 2025

The aim of this study was to investigate the survival benefits postoperative radiotherapy (PORT) in patients with resectable esophageal cancer (EC) after neoadjuvant therapy Immunotherapy era. designed as a retrospective cohort study, which included total 733 EC from SEER database and single-center cohort. We used propensity score matching (PSM) equilibrate patient characteristics. investigation incorporated Kaplan-Meier analysis Cox proportional risk regression model assess outcomes. PORT did not significantly improve overall cohort, median 38 months (p=0.56) 39 (p=0.75) Chinese However, immunotherapy subgroup, demonstrated that combined improved (p=0.044).Multivariate aged 50-59 years (HR=5.93, 95% CI: 1.67-21.06) those ≥70 (HR=10.96, CI:3.04-39.56) had increased risks compared <50 years. Additionally, ypT3-4 stage exhibited higher than ypT1-2 (HR=2.12, 1.14-3.93, p=0.017).Similar trends were observed cT3-4 staging, R1/R2 no immunotherapy. Lymph node metastasis also showed progressive relationship risk, categorized ypN1 (HR=1.90), ypN2 (HR=4.24), ypN3 (HR=6.68) experiencing increasingly (p<0.05). collaborative effect potentially enhances outcomes for EC. further prospective research is essential confirm our results.

Язык: Английский

Процитировано

0

Impact of Adjuvant Immunotherapy on Prognosis in Esophageal Squamous Cell Carcinoma Patients Following Neoadjuvant Immunochemotherapy DOI

Jiayi Geng,

Bengang Hui,

Teng Mu

и другие.

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Regulatory mechanisms of m6A RNA methylation in esophageal cancer: a comprehensive review DOI Creative Commons
Long Wen, Jiang Fu, Zixu Wang

и другие.

Frontiers in Genetics, Год журнала: 2025, Номер 16

Опубликована: Апрель 22, 2025

Esophageal cancer is an aggressively malignant neoplasm characterized by a high mortality rate. Frequently diagnosed at advanced stage, it presents challenges for optimal therapeutic intervention due to its non-specific symptoms, resulting in lost opportunities effective treatment, such as surgery, radiotherapy, chemotherapy and target therapy. The N6-methyladenosine (m6A) modification represents the most critical post-transcriptional of eukaryotic messenger RNA (mRNA). reversible m6A mediated three regulatory factors: methyltransferases, demethylating enzymes, recognition proteins. These components identify bind specific methylation sites, thereby modulating essential biological functions processing, nuclear export, stability, translation degradation, which significantly influence tumorigenesis, invasion, metastasis. Given importance modification, this paper offers comprehensive examination mechanisms, functions, future implications context esophageal cancer.

Язык: Английский

Процитировано

0

Circulating Tumor DNA Dynamic Changes in Esophageal Squamous Cell Carcinoma Receiving Immunochemotherapy DOI Creative Commons
Yijia Guo, Wang Qu, Yun Liu

и другие.

Thoracic Cancer, Год журнала: 2025, Номер 16(9)

Опубликована: Май 1, 2025

ABSTRACT Background Immune checkpoint inhibitors have revolutionized the treatment strategy of esophageal squamous cell carcinoma (ESCC). The value ctDNA dynamic changes in ESCC patients treated with immunochemotherapy was not clear. Methods A retrospective analysis performed to analyze association efficacy locally advanced, metastatic, or recurrent and who received at Department Medical Oncology, National Cancer Center from June 2023 December 2024. Tumor mutation burden (TMB) PD‐L1 expression tumor tissue were also explored. Results 57 paired baseline during analyzed. We found that negative demonstrated a higher regression rate (96.8% vs. 73.1%; p = 0.018) cCR (45.2% 15.4%; 0.022). Additionally, continuously ( 0.033) experienced clearance 0.043) had compared those persistently positive ctDNA. Moreover, among TP53 mutations baseline, cleared showed (88.9% 54.5%; 0.031) (33.3% 0%; 0.038) persistent mutations. No correlation observed between TMB efficacy, while CPS ≥ 15 (63.6% 24.4%; 0.027). Conclusions potential predictive for ESCC. Further exploration through larger‐scale studies is necessary.

Язык: Английский

Процитировано

0

Neoadjuvant chemotherapy with or without PD-1/PD-L1 inhibitors in resectable esophageal squamous cell carcinoma: a meta-analysis based on randomized controlled trials DOI Creative Commons
Ye Zhang, Jie Chen,

Fenglian Yu

и другие.

BMC Gastroenterology, Год журнала: 2025, Номер 25(1)

Опубликована: Май 29, 2025

Neoadjuvant chemotherapy (NC) is a cornerstone in the management of resectable esophageal squamous cell carcinoma (ESCC). The integration PD-1/PD-L1 inhibitors into NC (NIC) regimens has shown promise; however, its efficacy and safety remain uncertain. This meta-analysis aims to compare potential risks clinical benefits NIC versus patients with ESCC based on randomized controlled trials (RCTs). A thorough search six databases was performed identify RCTs evaluating ESCC. Key outcomes analyzed included pathological complete response (pCR) rate major (MPR) rate. Other overall survival (OS), event-free (EFS), surgery rate, R0 resection adverse events (AEs). Four encompassing 605 were included. significantly improved pCR (risk ratio [RR]: 2.66 [1.63, 4.34], P < 0.0001) MPR (RR: 1.74 [1.02, 2.95], = 0.04) compared group. Only one phase III RCT reported outcomes, showing that group demonstrated OS (HR: 0.48 [0.24, 0.96], EFS 0.62 [0.39, 0.99], 0.05). Additionally, 1.11 [1.03, 1.20], 0.008) number resected lymph nodes (mean difference [MD]: 3.91 [0.60, 7.21], 0.02) also higher duration surgery, intraoperative blood loss comparable between groups. However, immune-related AEs (irAEs) 40.80 [5.67, 293.37], 0.0002) Similar surgical complications observed two demonstrates superior improving alone, may potentially provide benefits, although it associated risk irAEs.

Язык: Английский

Процитировано

0

Tertiary lymphoid structures in esophageal cancer: a novel target for immunotherapy DOI Creative Commons
Kefeng Zhai, Yuting Ma, Xueqin Gao

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Июнь 4, 2025

Esophageal cancer (EC), especially esophageal squamous cell carcinoma (ESCC), which is the most common subtype in China, one of aggressive gastrointestinal malignancies. Traditional treatments like surgery, radiotherapy, and chemotherapy have limited success, but study tertiary lymphoid structure (TLS) has opened new avenues for immunotherapy. TLS an ectopic immune cluster, including B cells, T dendritic cells (DCs), that forms chronic inflammation such as tumors. often found at tumor invasion margins linked to better prognosis EC patients, with higher density maturation associated prolonged survival. can also serve a biomarker assess immunotherapy outcomes. Further exploration molecular mechanisms could innovate treatments, by inducing formation improve therapy. This review summarizes characteristics EC, clinical findings, limitations directions current research, offering insights future strategies.

Язык: Английский

Процитировано

0

Association Between Treatment-Related Adverse Event Trajectory and Prognosis in Esophageal Cancer Receiving Neoadjuvant Immunochemotherapy DOI
Zijie Li, Shujie Huang, Rixin Chen

и другие.

Annals of Surgical Oncology, Год журнала: 2024, Номер unknown

Опубликована: Дек. 27, 2024

Язык: Английский

Процитировано

1

Cancer-associated fibroblasts derived-exosomal circ_0076535 promotes esophageal squamous cell carcinoma progression DOI

Ningning Kang,

Wei Ge,

Jinxiu Hu

и другие.

Technology and Health Care, Год журнала: 2024, Номер unknown

Опубликована: Дек. 1, 2024

Background Esophageal cancer (EC) is a common malignant tumor of the digestive tract and an important health-related problem in many developing countries. squamous cell carcinoma (ESCC) most subtype EC. The cancer-associated fibroblasts (CAFs) are major stromal cells ESCC microenvironment. They play role proliferation, metastasis, angiogenesis chemotherapy resistance through paracrine processes. However, roles circRNAs enriched CAF-derived exosmes have not been reported. Objective To explore mechanisms how CAF affects proliferation metastasis processes to investigate exosomes. Methods Exosomes were isolated from conditional medium using differential ultracentrifugation, then validated by Nanosight analysis. Exosome secretion inhibitor-GW4869 validates pro-carcinogenic qRT-PCR showed highest expression circ_0076535 exosomal CircRNA, knockdown it confirmed its function. Online bioinformatics tool was utilized predict potential target gene circ_0076535, captured miR-145-5p as with high predictive value. targeting association between further dual luciferase reporter experiment. stimulation tumour development EMT exosome vivo. Results In our research, we found that exosomes increased migration, invasion cells. Circ_0076535 highly CAF-exosomes transferred into directly depend on internalization CAF-exosomal level induced EMT. Mechanistic experiments revealed acted sponge absorb activated NF-κB signaling pathway. Conclusions Conclusively, promoted progression via miR-145-5p/NF-κB axis expected be biomarker for early diagnosis treatment ESCC.

Язык: Английский

Процитировано

0