1,8-Cineole Alleviates Hippocampal Oxidative Stress in CUMS Mice via the PI3K/Akt/Nrf2 Pathway DOI Open Access

Wenze Wu,

Dequan Wang,

Yuzhu Shi

и другие.

Nutrients, Год журнала: 2025, Номер 17(6), С. 1027 - 1027

Опубликована: Март 14, 2025

Background: This study investigates the neuroprotective effects of 1,8-cineole (1,8-CH), against hippocampal oxidative stress in a chronic unpredictable mild (CUMS) mice model depression, focusing on underlying molecular mechanisms. Methods: The CUMS exposure were assessed by measuring markers, antioxidant activity, and neuronal damage hippocampus using histopathology, network pharmacology, Western blot analysis, small interfering RNA (siRNA) knockdown experiments. Results: 1,8-CH significantly alleviated depression-like behaviors mice. induced hippocampus, evidenced elevated MDA levels, decreased damage. DHE staining revealed ROS accumulation. Treatment with reducing MDA, restoring lowering while improving structure. Network pharmacology identified PI3K/Akt/Nrf2 pathway as key mediator 1,8-CH’s neuroprotection, which was supported results, demonstrating PI3K/Akt activation potential enhancement Nrf2 nuclear translocation. Furthermore, corticosterone-induced PC12 cells, abolished inhibition siRNA knockdown, confirming Nrf2’s role. Conclusions: These findings suggest that alleviates CUMS-induced depression via pathway, highlighting its health supplement for managing depression.

Язык: Английский

Seseli foliosum (Somm. et Levier) Manden.—A Comprehensive Phytochemical and Biological Evaluation DOI Creative Commons
Mariam Nersezashvili, Dali Berashvili,

Malkhaz Jokhadze

и другие.

Molecules, Год журнала: 2025, Номер 30(3), С. 725 - 725

Опубликована: Фев. 5, 2025

The genus Seseli L. (Apiaceae family) is widespread across Europe and Asia, with ten species identified in Georgia. Among these, foliosum (Somm. et Levier) Manden., notable for its unique pharmacological properties. To our knowledge, comprehensive phytochemical biological investigations have not yet been conducted. primary aim of this research to explore the chemical properties S. foliosum, thereby enhancing potential applications medicine related fields. Different chromatographic techniques were utilized isolate individual compounds identify composition MeOH Et2O extracts from seeds roots. A battery assays (antimicrobial, antioxidant, enzymatic, anxiolytic, cytotoxic) employed assess extracts. results gas chromatography mass spectrometry (GC/MS) revealed that both contain a diverse array compounds, including monoterpenoids, sesquiterpenoids, phenolic compounds. Furanocoumarin edultin was isolated extract by liquid–liquid separation (LLS). exhibited important enzyme inhibitory, antimicrobial activities efficacy against Staphylococcus aureus (MIC 125 µg/mL) Candida glabrata 62.5 µg/mL). Underground showed advanced cytotoxic activity, particularly hypopharyngeal carcinoma cells (CC50 22.33 µg/mL 27.16 µg/mL, respectively). study provides wide-range analysis highlighting as source bioactive These findings contribute understanding therapeutic lay groundwork further clinical research.

Язык: Английский

Процитировано

1

1,8-Cineole Alleviates Hippocampal Oxidative Stress in CUMS Mice via the PI3K/Akt/Nrf2 Pathway DOI Open Access

Wenze Wu,

Dequan Wang,

Yuzhu Shi

и другие.

Nutrients, Год журнала: 2025, Номер 17(6), С. 1027 - 1027

Опубликована: Март 14, 2025

Background: This study investigates the neuroprotective effects of 1,8-cineole (1,8-CH), against hippocampal oxidative stress in a chronic unpredictable mild (CUMS) mice model depression, focusing on underlying molecular mechanisms. Methods: The CUMS exposure were assessed by measuring markers, antioxidant activity, and neuronal damage hippocampus using histopathology, network pharmacology, Western blot analysis, small interfering RNA (siRNA) knockdown experiments. Results: 1,8-CH significantly alleviated depression-like behaviors mice. induced hippocampus, evidenced elevated MDA levels, decreased damage. DHE staining revealed ROS accumulation. Treatment with reducing MDA, restoring lowering while improving structure. Network pharmacology identified PI3K/Akt/Nrf2 pathway as key mediator 1,8-CH’s neuroprotection, which was supported results, demonstrating PI3K/Akt activation potential enhancement Nrf2 nuclear translocation. Furthermore, corticosterone-induced PC12 cells, abolished inhibition siRNA knockdown, confirming Nrf2’s role. Conclusions: These findings suggest that alleviates CUMS-induced depression via pathway, highlighting its health supplement for managing depression.

Язык: Английский

Процитировано

0