The Celiac‐Disease Superantigen Oligomerizes and Increases Permeability in an Enterocyte Cell Model
Angewandte Chemie International Edition,
Год журнала:
2024,
Номер
63(21)
Опубликована: Март 18, 2024
Celiac
disease
(CeD)
is
an
autoimmune
disorder
triggered
by
gluten
proteins,
affecting
approximately
1
%
of
the
global
population.
The
33-mer
deamidated
gliadin
peptide
(DGP)
a
metabolically
modified
wheat-gluten
superantigen
for
CeD.
Here,
we
demonstrate
that
DGP
spontaneously
assembles
into
oligomers
with
diameter
24
nm.
present
two
main
secondary
structural
motifs-a
major
polyproline
II
helix
and
minor
β-sheet
structure.
Importantly,
in
presence
oligomers,
there
statistically
significant
increase
permeability
gut
epithelial
cell
model
Caco-2,
accompanied
redistribution
zonula
occludens-1,
master
tight
junction
protein.
These
findings
provide
novel
molecular
supramolecular
insights
impact
CeD
highlight
relevance
oligomerization.
Язык: Английский
Thin‐Plate Superstructures of the Immunogenic 33‐mer Gliadin Peptide
ChemBioChem,
Год журнала:
2022,
Номер
23(22)
Опубликована: Сен. 26, 2022
Gluten
related-disorders
have
a
prevalence
of
1-5
%
worldwide
triggered
by
the
ingestion
gluten
proteins
in
wheat,
rye,
barley,
and
some
oats.
In
wheat
gluten,
most
studied
protein
is
gliadin,
whose
immunodominant
33-mer
amino
acid
fragment
remains
after
digestive
proteolysis
accumulates
gut
mucosa.
Here,
we
report
formation
thin-plate
superstructures
using
intrinsic
tyrosine
(Tyr)
steady-state
fluorescence
anisotropy
cryo-TEM
combination
with
water
tension
measurements.
Furthermore,
showed
that
decay
measurements
fluorophore
Tyr
provided
information
on
early
stages
structures.
Finally,
conformational
analysis
residues
minimalist
models
molecular
dynamic
simulations
(MD)
demonstrated
changes
rotamer
states
depend
oligomerization
stage.
Our
findings
further
advance
understanding
gliadin
peptide
their
relation
to
health
disease.
Язык: Английский
The Versatile Photo-Thermal Behaviour of a 2-Hydroxyazobenzene
Molecules,
Год журнала:
2023,
Номер
28(3), С. 1183 - 1183
Опубликована: Янв. 25, 2023
Photochromic
compounds
are
employed
in
implementing
neuron
surrogates.
They
will
boost
the
development
of
neuromorphic
engineering
wetware.
In
this
work,
photochromic
behaviours
(E)-3,4,6-trichloro-2-(p-diazenil)-phenol
(t-DZH)
and
its
conjugated
phenoxide
base
(t-DZ)
have
been
investigated
experimentally
three
different
media:
(1)
pure
acetonitrile,
(2)
water
acetonitrile
mixed
a
1/1
volume
ratio,
(3)
an
aqueous
micellar
solution
3-(N,N-Dimethylmyristylammonio)propanesulfonate
(SB3-14).
The
analysis
spectral
kinetic
features
t-DZH
t-DZ
has
supported
by
quantum-mechanical
DFT
calculations,
maximum
entropy
method,
determination
their
colourability
(C).
versatility
makes
them
promising
molecular
probes
micro-environments
potential
ingredients
photochemical
oscillators
required
for
pacemaker
neurons
capable
communicating
through
optical
signals
Язык: Английский
Das Zöliakie‐Superantigen Oligomerisiert und Erhöht die Permeabilität in einem Enterozyten‐Zellmodell
Angewandte Chemie,
Год журнала:
2024,
Номер
136(21)
Опубликована: Март 18, 2024
Abstract
Zöliakie
(CeD)
ist
eine
Autoimmunerkrankung,
die
durch
Glutenproteine
ausgelöst
wird
und
von
der
etwa
1
%
Weltbevölkerung
betroffen
ist.
Das
33‐mer
deamidierte
Gliadinpeptid
(DGP)
ein
metabolisch
modifiziertes
Weizengluten‐Superantigen
für
CeD.
Hier
zeigen
wir,
dass
das
DGP
spontan
zu
Oligomeren
mit
einem
Durchmesser
24
nm
aggregiert.
Die
33‐mer‐DGP‐Oligomere
weisen
zwei
sekundäre
Hauptstrukturmotive
auf:
Eine
große
Polyprolin‐II‐Helix
kleine
β‐Faltblattstruktur.
Wichtig
ist,
in
Anwesenheit
33‐mer‐DGP‐Oligomeren
statistisch
signifikante
Erhöhung
Permeabilität
im
Caco‐2
Darmepithelzellmodell
auftritt,
begleitet
einer
Umverteilung
Zonula
occludens‐1,
Haupt‐Tight‐Junction‐Protein.
Diese
Ergebnisse
bieten
neue
molekulare
supramolekulare
Einblicke
Auswirkungen
bei
CeD
unterstreichen
Bedeutung
Gliadin‐Peptid‐Oligomerisierung.