Recent Developments and Challenges in the Enzymatic Formation of Nitrogen–Nitrogen Bonds
ACS Catalysis,
Год журнала:
2024,
Номер
15(1), С. 310 - 342
Опубликована: Дек. 17, 2024
The
biological
formation
of
nitrogen–nitrogen
(N–N)
bonds
represents
intriguing
reactions
that
have
attracted
much
attention
in
the
past
decade.
This
interest
has
led
to
an
increasing
number
N–N
bond-containing
natural
products
(NPs)
and
related
enzymes
catalyze
their
(referred
this
review
as
NNzymes)
being
elucidated
studied
greater
detail.
While
more
detailed
information
on
biosynthesis
NPs,
which
only
become
available
recent
years,
provides
unprecedented
source
biosynthetic
enzymes,
potential
for
biocatalytic
applications
been
minimally
explored.
With
review,
we
aim
not
provide
a
comprehensive
overview
both
characterized
NNzymes
hypothetical
biocatalysts
with
putative
bond
forming
activity,
but
also
highlight
from
perspective.
We
present
compare
conventional
synthetic
approaches
linear
cyclic
hydrazines,
hydrazides,
diazo-
nitroso-groups,
triazenes,
triazoles
allow
comparison
enzymatic
routes
via
these
functional
groups.
Moreover,
pathways
well
diversity
reaction
mechanisms
are
presented
according
direct
groups
currently
accessible
enzymes.
Язык: Английский
Production of Phenyldiazene Derivatives Using the Biosynthetic Pathway of an Aromatic Diazo Group‐Containing Natural Product from an Actinomycete
ChemBioChem,
Год журнала:
2024,
Номер
26(1)
Опубликована: Окт. 18, 2024
Abstract
The
diazo
group
is
an
important
functional
in
organic
synthesis
because
it
confers
high
reactivity
to
the
compounds
and
has
been
applied
various
chemical
reactions,
such
as
Sandmeyer
reaction,
Wolff
rearrangement,
cyclopropanation,
C−N
bond
formation
with
active
methylene
compounds.
Previously,
we
revealed
that
3‐diazoavenalumic
acid
(3‐DAA),
which
potentially
produced
by
several
actinomycete
species
contains
aromatic
group,
a
biosynthetic
intermediate
of
avenalumic
acid.
In
this
study,
aimed
construct
production
system
for
phenyldiazene
derivatives
adding
culture
3‐DAA‐producing
recombinant
actinomycete.
First,
acetoacetanilide
its
derivatives,
have
are
raw
materials
arylide
yellow
dyes,
were
individually
added
When
their
metabolites
analyzed,
each
expected
compound
phenyldiazenyl
moiety
was
detected
extract.
Moreover,
established
one‐pot
vitro
enzymatic
same
using
highly
reactive
diazotase,
CmaA6.
These
results
showed
natural
products
attractive
tool
expanding
structural
diversity
both
vivo
.
Язык: Английский