Construction of an electrochemical aptasensor based on a covalent organic framework for rapid and ultrasensitive detection of staphylococcus aureus

Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137224 - 137224

Опубликована: Янв. 1, 2025

Язык: Английский

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Recent Advances in Aptamer-Based Biosensors for Bacterial Detection

Biosensors, Год журнала: 2024, Номер 14(5), С. 210 - 210

Опубликована: Апрель 23, 2024

The rapid and sensitive detection of pathogenic bacteria is becoming increasingly important for the timely prevention contamination treatment infections. Biosensors based on nucleic acid aptamers, integrated with optical, electrochemical, mass-sensitive analytical techniques, have garnered intense interest because their versatility, cost-efficiency, ability to exhibit high affinity specificity in binding bacterial biomarkers, toxins, whole cells. This review highlights development structural characterization, chemical modifications enabling optimized recognition properties enhanced stability complex biological matrices. Furthermore, recent examples aptasensors cells, toxins are discussed. Finally, we explore barriers discuss perspectives application aptamer-based detection.

Язык: Английский

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Multiple bacteria recognition mechanisms and their applications

Coordination Chemistry Reviews, Год журнала: 2024, Номер 517, С. 216025 - 216025

Опубликована: Июнь 18, 2024

Язык: Английский

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Decoding aptamer-protein binding kinetics for continuous biosensing using single-molecule techniques

Science Advances, Год журнала: 2025, Номер 11(7)

Опубликована: Фев. 14, 2025

Continuous biosensing provides real-time information about biochemical processes and holds great potential for health monitoring. Aptamers have emerged as promising alternatives over traditional biorecognition elements. However, the underlying aptamer-target binding interactions are often poorly understood. Here, we present a technique that can decode aptamer-protein at single-molecule level. We demonstrate our assay is able to kinetics of aptamers despite their similar affinity. Guided by computational simulations validated with quartz crystal microbalance experiments, show quantitative insights generated this enabled rational understanding biosensor performance (i.e., sensitivity limit detection). This capability was demonstrated thrombin analyte structurally HD1, RE31, NU172 work decodes high temporal resolution, paving way design aptamer-based biosensors.

Язык: Английский

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N-Doped Porous Carbon Synergistic Freezing-Induced DNA with Catalyzed Hairpin Assembly Enables Electrochemical One-Pot Detection of Pathogen in Food Samples

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 7, 2025

A DNA electrochemical interface biosensor based on screen-printed carbon electrodes (SPCEs) holds promise for point-of-care testing (POCT) detection of pathogens in food safety. Nevertheless, SPCE commonly has a rough surface and suffers from relatively low electron transfer rate, disorder capture probes (CPs), the steric hindrance effect target nucleic acid binding. These issues lead to sensitivity. Herein, simple rapid N-doped porous (NPC)-modified freezing-directed combined with catalyzed hairpin assembly (CHA) was constructed one-pot samples without time-consuming growth cultures. The by modified NPC enhanced properties, CP designed CHA stably fixed electrode high hybridization efficiency. Moreover, signals amplified enable selective sensitive washing steps. This method is wide linear range 101 107 CFU/mL limit 5 Escherichia coli shows specificity against other coexisting pathogens. whole complex performed only within 60 min sample-to-answer, which great potential POCT

Язык: Английский

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Ultrasensitive Detection of Extracellular Vesicles Based on Metal–Organic Framework DNA Biobarcodes Triggered G-Quadruplex Coupled with Rolling Circle Amplification Assay

ACS Sensors, Год журнала: 2025, Номер unknown

Опубликована: Март 6, 2025

Extracellular vesicles (EVs), as liquid biopsy markers for accurate tumor diagnosis, are considered to hold great promise. However, effectively isolating and sensitively detecting EVs with convenience still face challenges. Herein, we propose a highly sensitive specific platform EV detection by integrating metal-organic framework (MOF)-based DNA biobarcodes strategy rolling circle amplification (RCA)/G-quadruplex system. In this study, first, Zr-MOFs act signal converters comodification barcodes antibodies, converting amplifying the abundance of into barcodes. Second, released can trigger RCA, followed G-quadruplex formation further amplify signal. Consequently, approach significantly enhances sensitivity biomarker detection, achieving low limit 100 mL-1. Furthermore, offers high sensitivity, specificity, accuracy, simplicity, highlighting its potential clinical applications in noninvasive detection.

Язык: Английский

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Entropy spontaneous ratiometric electrochemical aptasensor based on polymerization and AuNPs signal amplification for Acetamiprid residue analysis

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 26, 2025

Acetamiprid (ACE), a next-generation chlorinated neonicotinoid insecticide, has been extensively employed for pest control. However, its excessive residues in food and the environment have raised significant concerns regarding human health. To address need simple, accurate, efficient ACE detection method, this study developed ratiometric electrochemical aptasensor utilizing dual signal amplification strategy involving atom transfer radical polymerization (ATRP) gold nanoparticles (AuNPs). Methylene blue (MB) served as internal reference signal, which was attached to aptamer-DNA₁ (dsDNA) through electrostatic adsorption intercalation. Ferrocenyl methyl methacrylate (FMMA) polymerized into long chains via ATRP, generating significantly amplified electrical compared that of monomeric FMMA. AuNPs, known their bioconjugability, were linked DNA₂ (AuNPs-DNA₂), enhancing loading capacity expanding ATRP reaction sites. In presence ACE, MB decreased while FMMA polymer increased, achieving with limit (LOD) 19.26 pg/mL. This not only enhances selectivity but also mitigates influence background currents, offering novel effective approach real-world sample analysis.

Язык: Английский

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Exonuclease III-assisted CRISPR/Cas12a electrochemical biosensor for detection of transcription factor binding catalytic hairpin assembly signal amplification technology

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113528 - 113528

Опубликована: Апрель 1, 2025

Язык: Английский

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A Hybridization Chain Reaction-Based Electrochemical Biosensor for Highly Sensitive and Selective Detection of miR-378

Journal of Analytical Chemistry, Год журнала: 2024, Номер 79(9), С. 1329 - 1337

Опубликована: Сен. 1, 2024

Язык: Английский

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