Development and applications of enzymatic peptide and protein ligation

Journal of Peptide Science, Год журнала: 2024, Номер unknown

Опубликована: Окт. 21, 2024

Chemical synthesis of complex peptides and proteins continues to play increasingly important roles in industry academia, where strategies for covalent ligation two or more peptide fragments produce longer convergent manners have become critical. In recent decades, efficient site‐selective mediated by exploiting the biocatalytic capacity nature's diverse toolkit (i.e., enzymes) been widely recognized as a powerful extension existing chemical strategies. this review, we present chronological overview development proteases, transpeptidases, transglutaminases, ubiquitin ligases. We survey different properties between reactions various enzymes, including selectivity efficiency reaction, “scar” left product, type amide bond formed (natural isopeptide), synthetic availability reactants, whether enzymes are orthogonal another. This review also describes how inherent specificity these can be exploited protein ligation.

Язык: Английский

Biomimetic folding strategies for chemical synthesis of disulfide‐bonded peptides and proteins

ChemBioChem, Год журнала: 2024, Номер unknown

Опубликована: Окт. 2, 2024

Disulfide-bonded peptides and proteins, including hormones, toxins, growth factors, others, are abundant in living organisms. These molecules play crucial physiological roles such as regulating cell organism growth, development, metabolism. They have also found widespread applications drugs or tool biomedical pharmaceutical research. However, the chemical synthesis of disulfide-bonded proteins is complicated by challenges associated with their folding. This review focuses on latest advancements peptide protein folding technologies. Particularly, it highlights biomimetic strategies that emulate naturally occurring oxidative processes nature. include chaperone-assisted folding, glycosylation-assisted organic-based methods. The anticipates future directions technology. Such research offers innovative approaches for complex otherwise difficult to fold.

Язык: Английский

One-step solid-phase synthesis of activated cell-penetrating peptides for cytosolic delivery of protein

Tetrahedron Letters, Год журнала: 2024, Номер unknown, С. 155385 - 155385

Опубликована: Ноя. 1, 2024

Язык: Английский