Label‐Free Multiphoton Imaging Reveals Volumetric Shifts Across Development in Sensory‐Related Brain Regions of a Miniature Transparent Vertebrate
The Journal of Comparative Neurology,
Год журнала:
2025,
Номер
533(4)
Опубликована: Апрель 1, 2025
ABSTRACT
Animals
integrate
information
from
different
sensory
modalities
as
they
mature
and
perform
increasingly
complex
behaviors.
This
may
parallel
differential
investment
in
specific
brain
regions
depending
on
the
changing
demands
of
inputs.
To
investigate
developmental
changes
volume
canonical
regions,
we
used
third
harmonic
generation
imaging
for
morphometric
analysis
forebrain
midbrain
larval
through
juvenile
adult
stages
Danionella
dracula
,
a
transparent,
miniature
teleost
fish
whose
is
optically
accessible
throughout
its
lifespan.
Relative
to
whole‐brain
volume,
increased
or
telencephalon,
higher
order
integration
center,
shows
most
dramatic
increases
between
30–60
days
postfertilization
(dpf)
again
at
90
dpf
animals
reach
adulthood.
The
torus
longitudinalis
(TL),
visuomotor
also
significantly
60
dpf.
In
contrast,
optic
tectum
(TeO),
retinal‐recipient
target,
progressively
decreases
30
dpf,
whereas
relatively
consistent
across
all
semicircularis
(TS),
secondary
auditory
mechanosensory
lateral
line
olfactory
bulb
(OB),
direct
target
epithelium.
sum,
higher‐order
centers
(telencephalon,
TL)
occurs
juveniles
adulthood
(60–90
dpf)
exhibit
more
cognitive
tasks,
modality‐dominant
earlier
(TeO)
development
(TS,
OB).
Complete
optical
access
’s
lifespan
provides
unique
opportunity
how
structure
over
correlates
with
connectivity,
microcircuitry,
behavior.
Язык: Английский
Danionella fishes
Nature Methods,
Год журнала:
2024,
Номер
21(10), С. 1767 - 1769
Опубликована: Окт. 1, 2024
Язык: Английский
Label-free multiphoton imaging reveals volumetric shifts across development in sensory-related brain regions of a miniature transparent vertebrate
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 22, 2024
ABSTRACT
Animals
integrate
information
from
different
sensory
modalities
as
they
mature
and
perform
increasingly
complex
behaviors.
This
may
parallel
differential
investment
in
specific
brain
regions
depending
on
the
demands
of
changing
inputs.
To
investigate
developmental
changes
volume
canonical
integration
regions,
we
used
third
harmonic
generation
imaging
for
morphometric
analysis
forebrain
midbrain
5
to
90
days
post
fertilization
(dpf)
Danionella
dracula
,
a
transparent,
miniature
teleost
fish
whose
is
optically
accessible
throughout
its
lifespan.
Relative
whole
volume,
increased
or
telencephalon,
higher
order
center,
torus
longitudinalis
(TL),
visuomotor
relatively
consistent
30
dpf,
until
it
increases
at
60
followed
by
another
increase
animals
reach
adulthood.
In
contrast,
optic
tectum
(TeO),
retinal-recipient
target,
progressively
decreases
30-90
whereas
across
all
stages
semicircularis
(TS),
secondary
auditory
mechanosensory
lateral
line
olfactory
bulb
(OB),
direct
target
epithelium.
sum,
centers
(telencephalon,
TL)
occurs
juveniles
adulthood
exhibit
more
cognitive
tasks,
modality-dominant
earlier
(TeO)
development
(TS,
OB).
Complete
optical
access
’s
lifespan
provides
unique
opportunity
how
structure
over
correlates
with
connectivity,
microcircuitry,
behavior.
Язык: Английский