Evaluation of the Binding Affinity of Nitrosylcobalamin to Intrinsic Factor as a Predictive Model for Cobalamin Binding Protein Interactions: A Comparative Study with Hydroxocobalamin DOI Creative Commons

Annette M. Sysel,

Joseph A. Bauer

Frontiers in Bioscience-Elite, Год журнала: 2025, Номер 17(1)

Опубликована: Март 18, 2025

Background: Intrinsic factor (IF) is a glycoprotein crucial for cobalamin (vitamin B12) absorption in the human body. This study aimed to evaluate binding affinity of nitrosylcobalamin (NO-Cbl), analog, recombinant IF derived from plants, using hydroxocobalamin (OH-Cbl) as comparative standard. Methods: Surface plasmon resonance (SPR) was employed assess kinetic parameters NO-Cbl and OH-Cbl interactions with plant- across various concentrations. Results: SPR analysis demonstrated that exhibited high affinities IF, equilibrium dissociation constant (KD) values picomolar range. showed slightly stronger (KD = 4.79 × 10-11 M) than 8.58 M). Despite both being bound differences stability were observed, particularly at higher Conclusion: Variations between may be attributed saturation sites or recognition issues specific plant-derived IF. underscores potential targeted therapeutic agent capable leveraging natural uptake pathways. These results also highlight suitability model predicting biological activity analogs despite nuanced native

Язык: Английский

Proteins and DNA Sequences Interacting with Tanshinones and Tanshinone Derivatives DOI Open Access
Piotr Szymczyk, M. Majewska, Jadwiga Nowak

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(2), С. 848 - 848

Опубликована: Янв. 20, 2025

Tanshinones, biologically active diterpene compounds derived from Salvia miltiorrhiza, interact with specific proteins and DNA sequences, influencing signaling pathways in animals humans. This study highlights tanshinone–protein interactions observed at concentrations achievable vivo, ensuring greater physiological relevance compared to vitro studies that often employ supraphysiological ligand levels. Experimental data suggest while tanshinones multiple proteomic targets, only a few enzymes are significantly affected relevant concentrations. apparent paradox may be resolved by tanshinones’ ability bind influence involved gene expression or mRNA stability, such as RNA polymerase II human antigen R protein. These trigger secondary, widespread changes expression, leading complex alterations. Although the current understanding of remains incomplete, this provides foundation for deciphering molecular mechanisms underlying therapeutic effects S. miltiorrhiza diterpenes. Additionally, numerous tanshinone derivatives have been developed enhance pharmacokinetic properties biological activity. However, their safety profiles remain poorly characterized, limiting comprehensive insights into medicinal potential. Further investigation is essential fully elucidate toxicological both native modified tanshinones.

Язык: Английский

Процитировано

0
A Photonic Crystal Fibre Sensor for the Detection of Biochemical Analytes With Superior Sensitivity DOI Creative Commons

S. M. Azmain Awsaf,

Fariba Tabassum Dola,

Jubair Mahamud Apon

и другие.

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 3, 2025

Abstract In this work we present a numerical study and experimental realization of new, simple highly sensitive surface plasmon resonance (SPR) biosensor with photonic crystal fiber (PCF). We have improved the performance our sensor by strategic patterning circular air holes within fiber. All characteristics were evaluated utilizing finite element method (FEM) based software COMSOL Multiphysics. A plasmonic material, gold (Au) layer, surrounded Finally, after optimizing all parameters fiber, achieved maximum amplitude sensitivity (AS) 2202.64 RIU− 1 wavelength (WS) 140,500 nm/RIU resolution 7.12 × 10− 7 for 4.54 4 amplitude. Furthermore, figure merit (FOM) is 2341.67. Fabrication tolerance limits are ± 5% layer variation 2.5% holes, overall biochemical analyte sensing range between refractive indices 1.31 1.40. expect SPR PCF to better utility in detection unknown analytes, medical diagnosis.

Язык: Английский

Процитировано

0
Flaxseed-derived peptide, Ile-Pro-Pro-Phe (IPPF), ameliorates hepatic cholesterol metabolism to treat metabolic dysfunction-associated steatotic liver disease by promoting cholesterol conversion and excretion DOI
Xingyu Yuan, Yifeng Hou, Nan Qin

и другие.

Food & Function, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

IPPF ameliorates hepatic cholesterol metabolism to treat MASLD.

Язык: Английский

Процитировано

0
Evaluation of the Binding Affinity of Nitrosylcobalamin to Intrinsic Factor as a Predictive Model for Cobalamin Binding Protein Interactions: A Comparative Study with Hydroxocobalamin DOI Creative Commons

Annette M. Sysel,

Joseph A. Bauer

Frontiers in Bioscience-Elite, Год журнала: 2025, Номер 17(1)

Опубликована: Март 18, 2025

Background: Intrinsic factor (IF) is a glycoprotein crucial for cobalamin (vitamin B12) absorption in the human body. This study aimed to evaluate binding affinity of nitrosylcobalamin (NO-Cbl), analog, recombinant IF derived from plants, using hydroxocobalamin (OH-Cbl) as comparative standard. Methods: Surface plasmon resonance (SPR) was employed assess kinetic parameters NO-Cbl and OH-Cbl interactions with plant- across various concentrations. Results: SPR analysis demonstrated that exhibited high affinities IF, equilibrium dissociation constant (KD) values picomolar range. showed slightly stronger (KD = 4.79 × 10-11 M) than 8.58 M). Despite both being bound differences stability were observed, particularly at higher Conclusion: Variations between may be attributed saturation sites or recognition issues specific plant-derived IF. underscores potential targeted therapeutic agent capable leveraging natural uptake pathways. These results also highlight suitability model predicting biological activity analogs despite nuanced native

Язык: Английский

Процитировано

0