In Vitro Models of Cardiovascular Disease: Embryoid Bodies, Organoids and Everything in Between DOI Creative Commons
Theodora M. Stougiannou, Konstantinos Christodoulou, Dimos Karangelis

и другие.

Biomedicines, Год журнала: 2024, Номер 12(12), С. 2714 - 2714

Опубликована: Ноя. 27, 2024

Cardiovascular disease comprises a group of disorders affecting or originating within tissues and organs the cardiovascular system; most, if not all, will eventually result in cardiomyocyte dysfunction death, negatively impacting cardiac function. Effective models are thus important for understanding crucial aspects progression, while recent advancements stem cell biology have allowed use populations to derive such models. These include three-dimensional (3D) as cell-based embryos (SCME) well organoids, many which frequently derived from embryoid bodies (EB). Not only can they recapitulate 3D form function, but developmental programs governing self-organization into more complex well. Many different organoids SCME constructs been generated years recreate tissue that give rise its cellular composition unique morphology. It is purpose this narrative literature review describe summarize recently organoid their recapitulation genetic acquired disease. Owing examined, focus on injury associated with embryonic/fetal tissues.

Язык: Английский

Excitatory Cortical Neurons from CDKL5 Deficiency Disorder Patient-Derived Organoids Show Early Hyperexcitability Not Identified in Neurogenin2 Induced Neurons DOI Open Access

Madison R. Glass,

Dosh Whye, Nickesha C. Anderson

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 11, 2024

CDKL5 deficiency disorder (CDD) is a rare developmental and epileptic encephalopathy resulting from variants in cyclin-dependent kinase-like 5 (CDKL5) that lead to impaired kinase activity or loss of function. CDD one the most common genetic etiologies identified epilepsy cohorts. To study how impact human neuronal activity, gene expression morphology, patient-derived induced pluripotent stem cells their isogenic controls were differentiated into excitatory neurons using either an NGN2 induction protocol guided cortical organoid differentiation. Patient-derived both differentiation paradigms had decreased phosphorylated EB2, known molecular target CDKL5. Induced showed no detectable differences between cases network multielectrode array, MAP2+ neurite length, only two genes differentially expressed. However, increased synchrony weighted mean firing rate on array within first month maturation. lower HS3ST1, which may change extracellular matrix around synapse contribute hyperexcitability. Similar neurons, there length across patient-control cell lines. have poor specification while derived expressed markers, suggesting changes excitability are specific neurons. Examining mechanisms early hyperexcitability promising avenue for identification therapeutics.

Язык: Английский

Процитировано

1
In Vitro Models of Cardiovascular Disease: Embryoid Bodies, Organoids and Everything in Between DOI Creative Commons
Theodora M. Stougiannou, Konstantinos Christodoulou, Dimos Karangelis

и другие.

Biomedicines, Год журнала: 2024, Номер 12(12), С. 2714 - 2714

Опубликована: Ноя. 27, 2024

Cardiovascular disease comprises a group of disorders affecting or originating within tissues and organs the cardiovascular system; most, if not all, will eventually result in cardiomyocyte dysfunction death, negatively impacting cardiac function. Effective models are thus important for understanding crucial aspects progression, while recent advancements stem cell biology have allowed use populations to derive such models. These include three-dimensional (3D) as cell-based embryos (SCME) well organoids, many which frequently derived from embryoid bodies (EB). Not only can they recapitulate 3D form function, but developmental programs governing self-organization into more complex well. Many different organoids SCME constructs been generated years recreate tissue that give rise its cellular composition unique morphology. It is purpose this narrative literature review describe summarize recently organoid their recapitulation genetic acquired disease. Owing examined, focus on injury associated with embryonic/fetal tissues.

Язык: Английский

Процитировано

0