International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(23), С. 16861 - 16861
Опубликована: Ноя. 28, 2023
Modern
biocatalysis
requires
fast,
sensitive,
and
efficient
high-throughput
screening
methods
to
screen
enzyme
libraries
in
order
seek
out
novel
biocatalysts
or
enhanced
variants
for
the
production
of
chemicals.
For
instance,
synthesis
bio-based
furan
compounds
like
2,5-diformylfuran
(DFF)
from
5-hydroxymethylfurfural
(HMF)
via
aerobic
oxidation
is
a
crucial
process
industrial
chemistry.
Laccases,
known
their
mild
operating
conditions,
independence
cofactors,
versatility
with
various
substrates,
thanks
use
chemical
mediators,
are
appealing
candidates
catalyzing
HMF
oxidation.
Herein,
Schiff-based
polymers
based
on
coupling
DFF
1,4-phenylenediamine
(PPD)
have
been
used
set-up
colorimetric
assay
detecting
presence
different
reaction
mixtures.
This
method
may
be
employed
fast
enzymes
(Z'
values
ranging
0.68
0.72).
The
sensitivity
has
proved,
detection
(8.4
μM)
quantification
(25.5
limits
calculated.
Notably,
displayed
selectivity
enabled
measurement
kinetics
using
three
distinct
laccase-mediator
systems.
ACS Catalysis,
Год журнала:
2022,
Номер
12(16), С. 10080 - 10114
Опубликована: Авг. 4, 2022
Biobased
furans,
such
as
furfural,
5-hydroxymethylfurfural,
and
2,5-furandicarboxylic
acid,
are
recognized
the
top-value
platform
chemicals
in
valorization
of
biomass
to
chemicals,
fuels,
materials.
Biocatalysis
has
emerged
a
promising
technique
chemical
pharmaceutical
industries
because
exquisite
selectivity,
mild
reaction
conditions,
environmental
friendliness,
etc.
In
this
comprehensive
review,
we
summarize
recent
advances
(chemo)biocatalytic
conversion
biobased
based
on
types
including
oxidation,
reduction,
C–C
C–N
bonds
formation,
esterification,
amidation.
Particularly,
biocatalysts
their
catalytic
mechanisms/pathways
critically
discussed
allow
rational
design
construction
efficient
enzymes/multienzyme
systems/chemobiocatalytic
systems.
Besides,
various
biocatalyst
engineering
strategies
highlighted
intensify
processes.
Future
research
directions
proposed
expand
space
furans
well
improve
commercial
acceptance
biocatalytic
Green Chemistry,
Год журнала:
2023,
Номер
25(15), С. 5836 - 5857
Опубликована: Янв. 1, 2023
This
critical
review
covers
advances
in
the
preparation
of
important
polyester
monomers
terephthalic
acid
(TPA),
2,5-furandicarboxylic
(FDCA),
and
ethylene
glycol
(EG),
with
particular
focus
on
biocatalytic
approaches.
5-hydroxymethylfurfural
(HMF)
is
produced
upon
dehydration
of
C6
sugars
in
biorefineries.
As
the
product,
it
remains
either
aqueous
solutions,
or
situ
extracted
to
an
organic
medium
(biphasic
system).
For
subsequent
oxidation
HMF
2,5-furandicarboxylic
acid
(FDCA),
'media-agnostic'
catalysts
that
can
be
efficiently
used
different
conditions,
from
biphasic,
and
(microaqueous)
media,
are
interest.
Here,
concept
a
one-pot
biocatalytic
cascade
for
production
FDCA
was
reported,
using
galactose
oxidase
(GalOx)
formation
2,5-diformylfuran
(DFF),
followed
by
lipase-mediated
peracid
DFF
FDCA.
GalOx
maintained
its
catalytic
activity
exposure
range
solvents
with
only
1
%
(v/v)
water.
The
(DFF)
successfully
established
ethyl
acetate-based
biphasic
microaqueous
systems.
To
validate
concept,
reaction
conducted
at
5
water,
integrated
where
subsequently
oxidized
catalyzed
Candida
antarctica
lipase
B.
EFB Bioeconomy Journal,
Год журнала:
2023,
Номер
3, С. 100050 - 100050
Опубликована: Март 10, 2023
The
production
of
2,5-furandicarboxylic
acid
(FDCA)
as
a
biobased
commodity
chemical
has
gained
great
importance
over
the
last
years.
possibility
replacing
conventional
polyethylene
terephthalate
(PET)-based
plastics
by
furanoate
(PEF)
is
accelerating
applied
research
in
direction
novel
and
highly
productive
synthesis
routes
to
FDCA.
This
paper
explores
patent
activities
related
FDCA
production,
with
particular
emphasis
on
potential
role
that
enzymatic
catalytic
methods
may
have.
An
increasing
number
applications
(and
granted
ones)
have
been
disclosed
decade.
innovation
set
development
multi-step
processes,
involving
multi-enzymatic
chemoenzymatic
cascades,
or
using
whole-cells,
either
resting
(non-growing)
biocatalysts
living
organisms
fermentative
processes.
Moreover,
other
innovative
paths
use
substrates
different
from
fructose
5-hydroxymethylfurfural
(HMF),
such
gluconic
acid,
furfural.
Overall,
opportunities
for
exist,
albeit
current
metrics
biotechnological
remain
mostly
at
proof-of-concept
level.
Large
efforts
reach
industrial
targets
are
needed,
which
be
stimulated
less
severe
processing
conditions
expected
biotechnology,
leading
energy
savings,
by-product
formation,
valorization
crude
effluents.
Biotechnology for Biofuels and Bioproducts,
Год журнала:
2023,
Номер
16(1)
Опубликована: Ноя. 1, 2023
Abstract
Background
As
a
cost-effective
and
eco-friendly
approach,
biocatalysis
has
great
potential
for
the
transformation
of
5-hydroxymethylfurfural
(HMF)
into
2,5-furandicarboxylic
acid
(FDCA).
However,
compatibility
each
enzyme
in
cascade
reaction
limits
efficiency
HMF
to
FDCA.
Results
Coupled
with
an
alcohol
oxidase
from
Colletotrichum
gloeosporioides
(
Cgl
AlcOx),
this
study
aims
bacterial
laccase
Bacillus
pumilus
Bp
Lac)
enzymatic
(FDCA)
biosynthesis
(HMF).
Lac
showed
100%
selectivity
oxidation
generated
5-hydroxymethyl-2-furancarboxylic
(HMFCA).
AlcOx
was
capable
oxidizing
HMFCA
2-formyl-5-furancarboxylic
(FFCA).
Both
could
oxidize
FFCA
At
5
mM
scale,
complete
97.5%
yield
FDCA
achieved
by
coupling
reaction.
The
productivity
5.3
mg/L/h.
Notably,
alleviate
inhibitory
effect
on
activity
boost
Moreover,
scaled
up
40
times
volume,
titer
reached
2.6
58.77%
at
168
h.
Conclusions
This
work
provides
candidate
novel
insight
better
design
production.
