A general route to retooling hydrolytic enzymes toward plastic degradation

Cell Reports Physical Science, Год журнала: 2024, Номер 5(2), С. 101783 - 101783

Опубликована: Янв. 29, 2024

The accumulation of plastic waste in the environment is an ecological disaster that requires a plurality approaches to tackle. There therefore ever-pressing need close loop on production both conventional and bioderived plastics. In response, we propose generalizable biocatalysis engineering strategy enhance use enzymes depolymerize broad class To demonstrate this approach, have solubilized stabilized commonly available lipase B from Candida antarctica ionic liquids. doing so, unlock ability enzyme hydrolytically post-consumer poly(lactic acid) plastic, demonstrating full degradation within 24 h conversion monomer 40 at 90°C. This facile scalable modification allows for elevated operation temperatures combination with superior solvent capabilities liquids, providing blueprint enhancing capability any hydrolytic recycling.

Язык: Английский

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Biocatalysis in Water or in Non-Conventional Media? Adding the CO2 Production for the Debate

Molecules, Год журнала: 2023, Номер 28(18), С. 6452 - 6452

Опубликована: Сен. 6, 2023

Biocatalysis can be applied in aqueous media and different non-aqueous solutions (non-conventional media). Water is a safe solvent, yet many synthesis-wise interesting substrates cannot dissolved solutions, thus low concentrations are often applied. Conversely, non-conventional may enable higher substrate loadings but at the cost of using (fossil-based) organic solvents. This paper determines CO2 production-expressed as kg CO2·kg product-1-of generic biotransformations water media, assessing both upstream downstream. The key to reaching diminished environmental footprint type wastewater treatment implemented. If used chemicals conventional (mild) treatment, production limited. other (pre)treatments for needed eliminate hazardous solvents, impacts expected (based on production). biocatalysis more sustainable during unit-the biocatalytic step-than systems. However, processes with need incorporate extractive solvents downstream processing. Both strategies result comparable if recycled least 1-2 times. Under these conditions, industrial biotransformation 100 g L-1 loading would produce 15-25 product-1 regardless media.

Язык: Английский

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Comparing a polynomial DOE model and an ANN model for enhanced geranyl cinnamate biosynthesis with Novozym® 435 lipase

Biocatalysis and Agricultural Biotechnology, Год журнала: 2024, Номер 58, С. 103240 - 103240

Опубликована: Май 17, 2024

Язык: Английский

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Biocatalytic approach for the synthesis of chiral alcohols for the development of pharmaceutical intermediates and other industrial applications: A review

Enzyme and Microbial Technology, Год журнала: 2024, Номер 180, С. 110483 - 110483

Опубликована: Июль 17, 2024

Язык: Английский

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Selective Biocatalytic Defunctionalization of Raw Materials

ChemSusChem, Год журнала: 2022, Номер 15(9)

Опубликована: Апрель 7, 2022

Biobased raw materials, such as carbohydrates, amino acids, nucleotides, or lipids contain valuable functional groups with oxygen and nitrogen atoms. An abundance of many the same type, primary secondary hydroxy in however, limits synthetic usefulness if similar reactivities cannot be differentiated. Therefore, selective defunctionalization highly functionalized biobased starting materials to differentially compounds can provide a sustainable access chiral synthons, even case products fewer groups. Selective reactions, without affecting other are fundamental interest for biocatalytic reactions. Controlled defunctionalizations attractive obtaining platform chemicals building blocks. The removal groups, an important feature natural metabolic pathways, also utilized systemic strategy metabolite synthesis.

Язык: Английский

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Responsive microgels and microgel assemblies in biocatalytic applications

Journal of Polymer Science, Год журнала: 2023, Номер 61(16), С. 1730 - 1748

Опубликована: Апрель 25, 2023

Abstract Microgels are soft particles that offer excellent colloidal stability, large surface area, and fluid‐like transport characteristics, making them ideal for enzyme immobilization reaction regulation. In recent years, the use of microgels in processes has rapidly increased reviewed. Here this review, we have provided a comprehensive overview strategies involved microgel synthesis, practical aspects microgel–enzyme conjugate formation, effects on stabilization activities. end, discussed important biocatalytic applications enzyme. The aim review is to provide detailed understanding enzyme–microgel conjugates their potential applications, which can aid design new microgels, responsive platforms, biochemical applications.

Язык: Английский

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The joint effort of enzyme technology and flow chemistry to bring biocatalytic processes to the next level of sustainability, efficiency and productivity

Journal of Flow Chemistry, Год журнала: 2023, Номер 14(1), С. 85 - 96

Опубликована: Ноя. 7, 2023

Abstract The number of biocatalyzed reactions at industrial level is growing rapidly together with our understanding on how we can maximize the enzyme efficiency, stability and productivity. While biocatalysis nowadays recognized as a greener way to operate in chemistry, its combination continuous processes has lately come up powerful tool enhance process selectivity, productivity sustainability. This perspective aims describing recent advances this technology future developments leading smart, efficient strategies for optimization large-scale production. Graphical

Язык: Английский

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Moving towards the Application of Biocatalysis in Food Waste Biorefinery

Fermentation, Год журнала: 2023, Номер 9(1), С. 73 - 73

Опубликована: Янв. 16, 2023

Waste valorization is an important strategy to reduce environmental pollution and dependency on petroleum-based fuels. In this regard, utilization of food waste as a versatile low-cost resource important. Several advanced catalytic methods for the have been widely investigated production liquid biofuels. Along line, chemical catalysts explored synthesis Chemo-catalysis mainly metal based, which requires harsh process conditions. Alternatively, biocatalysts are currently being result several advantages such mild reaction conditions, recyclability, selectivity biodegradability. work, recent biocatalytic technologies preparation biofuels through discussed thoroughly. Lipases employed biodiesel upgradation bio-oil, whereas methane mono-oxygenases could be methanol via oxidation generated from wastes. Industrial ethanol using bioconversion success story. To date, there has no specific report use propanol enzymes. The ABE (Acetone–Butanol–Ethanol) (using suitable microorganisms) used butanol preparation, where vacuum stripping system integrated remove broth circumvent inhibition. hydrocarbon fuels fatty acids triglycerides can carried out enzymes, carboxylic acid reductase photodecarboxylase (an algal photoenzyme). Both not yet applied in direct Furthermore, limitations reported methods, societal economic aspects fresh perspective subject, along with examples, described.

Язык: Английский

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Green solvents and biocatalysis: A bigger picture

EFB Bioeconomy Journal, Год журнала: 2023, Номер 3, С. 100056 - 100056

Опубликована: Июнь 7, 2023

Biocatalysis has matured to be used in an ample array of reaction media, from pure aqueous solutions (water-free) non-conventional media. In most the applications solvents are needed as media and/or during downstream unit extract and purify product. Aligning processes with Green Chemistry principles, some green have been introduced. This article discusses critically environmental impact that may a biocatalytic reaction, raw material extraction solvent synthesis its use biocatalysis ultimate disposal. Some observed impacts – quantified kg CO2·kg product−1 surely unavoidable, they depend on or used. However, intensification (e.g. using higher substrate loadings) investing efforts recycling clear final ecological footprint enzymatic process. Instead qualitatively stating used, experiments these directions should performed provide conditions minimized metrics for sustainable chemistry.

Язык: Английский

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Advancing PLP Biosynthesis: Enhanced Stability and Activity of EcPdxK via LXTE‐600 Immobilization

Biotechnology and Applied Biochemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 3, 2025

ABSTRACT Pyridoxal 5ʹ‐phosphate (PLP) plays an essential role in a multitude of cellular processes due to its function as critical coenzyme. This study introduces significant advancement PLP biosynthesis by enhancing the stability and activity Escherichia coli –derived pyridoxal kinase ( Ec PdxK) through immobilization on innovative epoxy resin, LXTE‐600. Our approach involved systematic optimization enzyme loading, coupling duration, temperature, which resulted improved efficiency high loading capacity 80 mg/g. The characterization immobilized PdxK@LXTE‐600 was conducted using Fourier transform infrared spectroscopy (FTIR) confocal laser scanning microscopy (CLSM), confirming successful immobilization. process notably enhanced enzyme's performance, increasing tolerance pH temperature fluctuations, thereby improving thermal stability. retained over 80% initial after 4 weeks storage at 4°C could be reused up eight cycles while maintaining more than 70% activity. These findings not only demonstrate efficacy LXTE‐600‐based method but also suggest promising industrial applications for sustainable production PLP, potentially revolutionizing approaches biotechnological pharmaceutical sectors.

Язык: Английский

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