Complexity reduction and opportunities in the design, integration and intensification of biocatalytic processes for metabolite synthesis

Frontiers in Bioengineering and Biotechnology, Год журнала: 2022, Номер 10

Опубликована: Июль 22, 2022

The biosynthesis of metabolites from available starting materials is becoming an ever important area due to the increasing demands within life science research area. Access making essential contributions analytical, diagnostic, therapeutic and different industrial applications. These molecules can be synthesized by enzymes biological systems under sustainable process conditions. facile synthetic access metabolite metabolite-like molecular space fundamental importance. knowledge biology, enzyme discovery production together with their biochemical structural properties offers excellent opportunities for using modular cell-free biocatalytic systems. This reduces complexity synthesizing whole-cell approaches or classical chemical synthesis. A biocatalysis approach provide a wealth optimized already identified new molecules.

Язык: Английский

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Route selection and reaction engineering for sustainable metabolite synthesis

Reaction Chemistry & Engineering, Год журнала: 2023, Номер 8(9), С. 2109 - 2118

Опубликована: Янв. 1, 2023

Great advances in tools and methodologies along the whole workflow are providing sustainable routes to a desired metabolite which can replace extractive manufacturing from endangered biological species or lengthy chemical fossil-based starting materials.

Язык: Английский

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Scalable Electrochemical Decarboxylative Olefination Driven by Alternating Polarity**

Angewandte Chemie, Год журнала: 2023, Номер 135(42)

Опубликована: Сен. 1, 2023

Abstract A mild, scalable (kg) metal‐free electrochemical decarboxylation of alkyl carboxylic acids to olefins is disclosed. Numerous applications are presented wherein this transformation can simplify alkene synthesis and provide alternative synthetic access valuable from simple acid feedstocks. This robust method relies on alternating polarity maintain the quality electrode surface local pH, providing a deeper understanding Hofer‐Moest process with unprecedented chemoselectivity.

Язык: Английский

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Industrial asymmetric biocatalysis

Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 431 - 463

Опубликована: Янв. 1, 2024

Язык: Английский

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Biomimetic Photoexcited Cobaloxime Catalysis in Organic Synthesis

Angewandte Chemie, Год журнала: 2024, Номер unknown

Опубликована: Май 22, 2024

Abstract Drawing inspiration from nature has long been a cornerstone of chemical innovation, with natural systems offering wealth untapped potential for discovery. In this minireview, we delve into the burgeoning field cobaloxime catalysis in organic synthesis, which mimics catalytic activity organometallic alkylcobalamine enzymes. Our focus lies on elucidating latest advancements area, as well delineating primary mechanistic pathways at play. By describing, and comparing these mechanisms, provide comprehensive overview current state‐of‐the‐art, while also shedding light key unresolved challenges that await further exploration.

Язык: Английский

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Enzyme Catalysis for Sustainable Value Creation Using Renewable Biobased Resources

Molecules, Год журнала: 2024, Номер 29(23), С. 5772 - 5772

Опубликована: Дек. 6, 2024

Enzyme catalysis was traditionally used by various human cultures to create value long before its basic concepts were uncovered. This achieved transforming the raw materials available from natural resources into useful products. Tremendous scientific and technological progress has been made globally in understanding what constitutes an enzyme; reactions enzymes can catalyze; how search, develop, apply, improve make desired The properties of as nature's preferred catalysts, such their high selectivity, diversity, adaptability, enable optimal function, whether single or multiple reactions. Excellent opportunities for resource-efficient manufacturing compounds are provided actions working reaction cascades pathways within same space, like molecular robots along a production line. plays increasingly prominent role industrial innovation responsible areas, green sustainable chemistry white biotechnology. Sources inspiration include current supply chain challenges, treasure enzymes, engineer tailor-made enzymes. Making best use power enzyme is essential changing products manufactured; renewable biobased replace fossil-based resources; improving safety, health, environmental aspects processes support cleaner more production.

Язык: Английский

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Synthesis of Metabolites and Metabolite-Like Compounds Using Biocatalytic Systems

Опубликована: Авг. 18, 2023

Methodologies for the synthesis and purification of metabolites, which have been developed following their discovery, analysis structural identification, involved in numerous life science milestones. The renewed focus on small molecule domain biological cells has also created an increasing awareness rising gap between metabolites identified prepared as pure compounds. Due to large number molecular diversity design engineering resource-efficient straightforward synthetic methodologies production attracted much interest. variety metabolic pathways provide a wonderful blueprint designing simplified routes desired metabolites. Therefore, biocatalytic systems become key enabling tools can then be utilized standards, enzyme substrates, inhibitors or products, discovery novel functions.

Язык: Английский

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Synthesis of Metabolites and Metabolite-like Compounds Using Biocatalytic Systems

Metabolites, Год журнала: 2023, Номер 13(10), С. 1097 - 1097

Опубликована: Окт. 19, 2023

Methodologies for the synthesis and purification of metabolites, which have been developed following their discovery, analysis, structural identification, involved in numerous life science milestones. The renewed focus on small molecule domain biological cells has also created an increasing awareness rising gap between metabolites identified prepared as pure compounds. design engineering resource-efficient straightforward synthetic methodologies production diverse metabolite-like compounds attracted much interest. variety metabolic pathways provides a wonderful blueprint designing simplified routes to desired metabolites. Therefore, biocatalytic systems become key enabling tools number can then be utilized standards, enzyme substrates, inhibitors, or other products, discovery novel functions.

Язык: Английский

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Herstellung von biobasiertem Ethylbenzol durch Kaskaden‐Biokatalyse mit einer speziell entwickelten Photodecarboxylase

Angewandte Chemie, Год журнала: 2023, Номер 136(8)

Опубликована: Ноя. 10, 2023

Abstract Die Herstellung von chemischen Grundstoffen wie Benzol, Toluol, Ethylbenzol und Xylol (BTEX) aus erneuerbaren Ressourcen ist der Schlüssel zu einer nachhaltigen Gesellschaft. Biokatalyse ermöglicht die Ein‐Topf‐Mehrschritt‐Transformation Bioressourcen unter milden Bedingungen, jedoch oft auf Biochemikalien beschränkt. Wir haben eine nicht‐natürliche Drei‐Enzym‐Kaskade für Ein‐Topf‐Umwandlung biobasiertem l ‐Phenylalanin in entwickelt. geschwindigkeitsbestimmende Photodecarboxylase als Schlüsselenzym wurde einem strukturbasierten semi‐rationalem Engineering unterzogen es Dreifachmutante CvFAP(Y466T/P460A/G462I) mit 6.3‐fach höheren Produktivität erhalten. Mit dieser verbesserten ein optimiertes sequentielles E. coli Zwei‐Zellsystem‐Verfahren zur Umwandlung 82 % Umsatz Kaskadenreaktion Fermentation integriert, um Ein‐Topf‐Bioproduktion Glycerin erreichen. Dies zeigt das Potenzial Kaskaden‐Biokatalyse Kombination Enzym‐Engineering Produktion biobasierten auf.

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