Engineering a vanillate-producing strain of Pseudomonas sp. NGC7 corresponding to aromatic compounds derived from the continuous catalytic alkaline oxidation of sulfite lignin DOI Creative Commons

M. KAMADA,

Chieko Yasuta,

Yudai Higuchi

и другие.

Microbial Cell Factories, Год журнала: 2024, Номер 23(1)

Опубликована: Ноя. 19, 2024

Lignin is a promising resource for obtaining aromatic materials, however, its heterogeneous structure poses challenge effective utilization. One approach to produce homogeneous materials from lignin involves the application of microbial catabolism, which gaining attention. This current study focused on constructing catabolic pathway in Pseudomonas sp. NGC7 vanillate (VA) compounds derived chemical depolymerization sulfite lignin. Alkaline oxidation was performed using hydroxide nanorod copper foam [Cu(OH)2/CF]-equipped flow reactor. The reactor operated continuously 50 h without clogging and it yielded stream containing acetovanillone (AV), vanillin (VN), VA as major monomers. optimized maximize production monomers this process. possesses four gene sets O-demethylase, comprising oxygenase component (vanA) oxidoreductase (vanB). Among these, vanA4B4 set identified key contributor catabolism. To facilitate conversion AV VA, AV-converting enzyme genes Sphingobium lignivorans SYK-6 were introduced. ΔvanA4B4 strain, harboring these genes, produced with 91 mol%. Further disruption vanA1B1, vanA2B2, vanA3B3, reductase gene, addition vanA4B4, introduction 5-carboxyvanillate decarboxylase S. utilize 5-carboxyvanillin achieved yield 103 Developing methods overcome heterogeneity essential use raw material. Consolidating continuous alkaline Cu(OH)2/CF-packed biological funneling an engineered synthesis. higher adaptability various generated natural ability grow p-hydroxyphenyl, guaiacyl, syringyl underscores potential bacterial chassis wide range lignin-derived compounds.

Язык: Английский

Guidelines for Identifying the Structure of Heavy Phenolics in Lignin Depolymerization by Using High‐Resolution Tandem Mass Spectrometry DOI
Cunhao Cui, Linyu Zhu, Zaifa Shi

и другие.

ChemSusChem, Год журнала: 2024, Номер unknown

Опубликована: Окт. 10, 2024

The efficient conversion of lignin contributes to reducing human reliance on fossil energy. As a complicated biopolymer, studies the mechanism depolymerization is limited by inadequate structural identification high molecular weight (MW) products like heavy phenolics. Up now, no individual method can generate both MW and information in operando conditions. promising approach, tandem mass spectrometry (MS/MS) techniques provide via dissociation target ions. In this study, MS/MS technique was performed offline in-situ mode during depolymerization. fundamental guidelines based principles for typical inter-unit linkages β-O-4, 5-5, β-β, β-5, β-1 were well established. Based that, major phenolic dimers are successfully identified, including chemical formula types linkages. More significantly, real-time monitoring evolution achieved applying analysis results show different pathways isomers with same formula, confirming that changes common obvious. Overall, study develops an advanced strategy full-view depolymerization, achieving static composition structure, dynamic structures.

Язык: Английский

Процитировано

2
Online Mass Spectrometric Characterization of Oligomeric Products in High-Pressure Liquid-Phase Lignin Depolymerization Reactions DOI Creative Commons
Zhongyue Zhou, Cunhao Cui, Linyu Zhu

и другие.

ACS Measurement Science Au, Год журнала: 2024, Номер 5(1), С. 9 - 18

Опубликована: Ноя. 18, 2024

Lignin depolymerization involves complex reactions that occur in heterogeneous environments, leading to the formation of a wide range products with diverse molecular structures. The complexity these arises from different bond strengths and locations within lignin polymer, which makes it difficult fully understand reaction pathways. Conventional analytical techniques often fall short providing clear comprehensive picture mechanism. This highlights need for more advanced methods can offer real-time, situ analysis probe product evolutions unravel detailed mechanisms depolymerization. Herein, we present concise perspective recent developments online mass spectrometry, particularly its applications probing heavy oligomeric formed during lignindepolymerization. After introducing current technologies challenges, focus on development spectrometric method, especially those combined batch flow-through reactors, real-time characterization products. Several key case studies are highlighted. Finally, discuss potential opportunities remaining challenges this field.

Язык: Английский

Процитировано

1
Engineering a vanillate-producing strain of Pseudomonas sp. NGC7 corresponding to aromatic compounds derived from the continuous catalytic alkaline oxidation of sulfite lignin DOI Creative Commons

M. KAMADA,

Chieko Yasuta,

Yudai Higuchi

и другие.

Microbial Cell Factories, Год журнала: 2024, Номер 23(1)

Опубликована: Ноя. 19, 2024

Lignin is a promising resource for obtaining aromatic materials, however, its heterogeneous structure poses challenge effective utilization. One approach to produce homogeneous materials from lignin involves the application of microbial catabolism, which gaining attention. This current study focused on constructing catabolic pathway in Pseudomonas sp. NGC7 vanillate (VA) compounds derived chemical depolymerization sulfite lignin. Alkaline oxidation was performed using hydroxide nanorod copper foam [Cu(OH)2/CF]-equipped flow reactor. The reactor operated continuously 50 h without clogging and it yielded stream containing acetovanillone (AV), vanillin (VN), VA as major monomers. optimized maximize production monomers this process. possesses four gene sets O-demethylase, comprising oxygenase component (vanA) oxidoreductase (vanB). Among these, vanA4B4 set identified key contributor catabolism. To facilitate conversion AV VA, AV-converting enzyme genes Sphingobium lignivorans SYK-6 were introduced. ΔvanA4B4 strain, harboring these genes, produced with 91 mol%. Further disruption vanA1B1, vanA2B2, vanA3B3, reductase gene, addition vanA4B4, introduction 5-carboxyvanillate decarboxylase S. utilize 5-carboxyvanillin achieved yield 103 Developing methods overcome heterogeneity essential use raw material. Consolidating continuous alkaline Cu(OH)2/CF-packed biological funneling an engineered synthesis. higher adaptability various generated natural ability grow p-hydroxyphenyl, guaiacyl, syringyl underscores potential bacterial chassis wide range lignin-derived compounds.

Язык: Английский

Процитировано

0