Immune profiling of the macroenvironment in colorectal cancer unveils systemic dysfunction and plasticity of immune cells DOI Creative Commons
Haoxian Ke,

Peisi Li,

Z. Y. Li

и другие.

Clinical and Translational Medicine, Год журнала: 2025, Номер 15(2)

Опубликована: Фев. 1, 2025

Abstract Background Tumour immune macroenvironment is comprised of tumour and surrounding organs responding to tumourigenesis immunotherapy. The lack comprehensive analytical methods hinders its application for prediction survival treatment response in colorectal cancer (CRC) patients. Methods Cytometry by time‐of‐flight (CyTOF) RNA‐seq was applied characterise cell heterogeneity a discovery cohort including tumour, blood intestinal architecture comprising epithelium, lamina propria, submucosa, muscularis propria normal bowel tumour–adjacent tissues. Immunoprofiling also validated validation using single‐cell RNA sequencing, spatial transcription, CyTOF multiplex immunofluorescent staining. Results Based on phenotype we identify distinct immunotypes the CRC blood, different architecture, showing disturbed compositions, increasing expression immunosuppressive markers cell–cell interactions contributing regulation. Furthermore, evaluate influencing factors tertiary lymphoid structures (TLSs), consensus molecular subtypes (CMSs) checkpoint inhibitors (ICIs). TLS presence fuels anti‐tumour immunity promoting CD8 + T infiltration altering activation or suppression systematically. correlates with patient survival, intrinsic CMS therapeutic efficacy ICI. PD‐1 CD69 expressed effector memory cells from can predict macroenvironment, serving as potential biomarkers stratifying patients into Conclusions Our findings provide insights highlighting tumourigenesis. study illustrates utility predicting immunotherapy response. Key points Distinct are identified macroenvironment. exert influence CD8+ Tem

Язык: Английский

Immune profiling of the macroenvironment in colorectal cancer unveils systemic dysfunction and plasticity of immune cells DOI Creative Commons
Haoxian Ke,

Peisi Li,

Z. Y. Li

и другие.

Clinical and Translational Medicine, Год журнала: 2025, Номер 15(2)

Опубликована: Фев. 1, 2025

Abstract Background Tumour immune macroenvironment is comprised of tumour and surrounding organs responding to tumourigenesis immunotherapy. The lack comprehensive analytical methods hinders its application for prediction survival treatment response in colorectal cancer (CRC) patients. Methods Cytometry by time‐of‐flight (CyTOF) RNA‐seq was applied characterise cell heterogeneity a discovery cohort including tumour, blood intestinal architecture comprising epithelium, lamina propria, submucosa, muscularis propria normal bowel tumour–adjacent tissues. Immunoprofiling also validated validation using single‐cell RNA sequencing, spatial transcription, CyTOF multiplex immunofluorescent staining. Results Based on phenotype we identify distinct immunotypes the CRC blood, different architecture, showing disturbed compositions, increasing expression immunosuppressive markers cell–cell interactions contributing regulation. Furthermore, evaluate influencing factors tertiary lymphoid structures (TLSs), consensus molecular subtypes (CMSs) checkpoint inhibitors (ICIs). TLS presence fuels anti‐tumour immunity promoting CD8 + T infiltration altering activation or suppression systematically. correlates with patient survival, intrinsic CMS therapeutic efficacy ICI. PD‐1 CD69 expressed effector memory cells from can predict macroenvironment, serving as potential biomarkers stratifying patients into Conclusions Our findings provide insights highlighting tumourigenesis. study illustrates utility predicting immunotherapy response. Key points Distinct are identified macroenvironment. exert influence CD8+ Tem

Язык: Английский

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