New insights into interspecies relationships, chromosomal evolution, and hybrid identification in the Lycoris Herb.

BMC Plant Biology, Год журнала: 2025, Номер 25(1)

Опубликована: Янв. 21, 2025

Frequent interspecific hybridization, unclear genetic backgrounds, and ambiguous evolutionary relationships within the genus Lycoris pose significant challenges to identification classification of hybrids, thereby impacting application development Lycoris. This study utilizes karyotype structure, genome size, fluorescent in situ hybridization (FISH) technology explore chromosomal evolution hybrid employing three approaches at cytogenetic level. The findings indicate that species with a smaller basic chromosome number exhibit less asymmetry than those larger number, suggesting different numbers may have followed pathways. aurea has more symmetrical karyotype, which be plesiomorphic state, reflecting an transition from symmetry chromosomes. Systematic clustering 18 is consistent classification, primarily dividing into two groups: M + T A type as one group, another group. average nuclear size (C-value) 22.99 Gb, smallest being L. wulingensis (17.10 Gb) largest squamigera (33.06 Gb). Chromosome length positively correlated C-value, haploid (Cx-value) decreases increase (x). FISH technique can quickly identify authenticate artificial thus inferring parentage natural hybrids. reveals background species, identifies authenticity infers possible offering technical insights for identification, genomic projects

Язык: Английский

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Development of tetraploids in tissue culture: modern techniques and biotechnological innovations

Plant Cell Tissue and Organ Culture (PCTOC), Год журнала: 2025, Номер 160(2)

Опубликована: Фев. 1, 2025

Язык: Английский

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A multidisciplinary and integrative review of the structural genome and epigenome of Capsicum L. species

Planta, Год журнала: 2025, Номер 261(4)

Опубликована: Март 9, 2025

Язык: Английский

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Expanding the toolkit for ploidy manipulation in Chlamydomonas reinhardtii

New Phytologist, Год журнала: 2025, Номер unknown

Опубликована: Март 21, 2025

Whole-genome duplications, widely observed in plant lineages, have significant evolutionary and ecological impacts. Yet, our current understanding of the direct implications ploidy shifts on short- long-term evolution remains fragmentary, necessitating further investigations across multiple levels. Chlamydomonas reinhardtii is a valuable model organism with profound potential to study impact increase longer term laboratory environment. This partly due ability level. We developed strategy engineer C. using noninterfering, antibiotic, selectable markers. approach allows us induce higher levels applicable field isolates, which expands beyond specific auxotroph strains broadens genetic diversity parental haploid that can be crossed. implement flow cytometry for precise measurement genome size different ploidy. demonstrate creation diploids, triploids, tetraploids by engineering North American broadening application synthetic biology principles reinhardtii. However, newly formed triploids show signs rapid aneuploidization. Our greatly facilitates polyploidy, both fundamental applied settings.

Язык: Английский

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MuPETFlow: multiple ploidy estimation tool from flow cytometry data

BMC Genomics, Год журнала: 2025, Номер 26(1)

Опубликована: Март 26, 2025

Ploidy, representing the number of homologous chromosome sets, can be estimated from flow cytometry data acquired on cells stained with a fluorescent DNA dye. This estimation relies combination tools that often require scripting, individual sample curation, and additional analyses. To automate ploidy for multiple files, we developed MuPETFlow—a Shiny graphical user interface tool. MuPETFlow allows users to visualize cell fluorescence histograms, detect peaks corresponding different cycle phases, perform linear regression using standards, make or genome size predictions, export results as figures table files. The tool was benchmarked known datasets yeast plant species, yielding consistent results. MuPETFlow's detection performance were also compared those other tools. stands out only offering in-app detection, peak multi-sample visualization, automation capabilities. These features significantly accelerate analysis, making it especially valuable projects involving large datasets.

Язык: Английский

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Reductional dysploidy and genome size diversity in Pooideae, the largest subfamily of grasses (Poaceae)

Plant Systematics and Evolution, Год журнала: 2025, Номер 311(3)

Опубликована: Май 9, 2025

Abstract The nuclear genome sizes of 59 species from 33 genera the Poaceae subfamily Pooideae were investigated by flow cytometry. This is characterized a wide range holoploid (2C values) and monoploid (1Cx mean chromosome sizes, including both highest some lowest values entire grass family. For example, tribe Brachypodieae has smallest genomes chromosomes, followed majority Stipeae individual representatives tribes Ampelodesmeae, Duthieeae Meliceae, which belong to phylogenetically ‘early-diverging’ lineages. Comparatively large found in Lygeeae Meliceae. ‘core Pooideae’ had largest subfamily, with greatest variation Aveneae, Festuceae Poeae. Bromeae especially Triticeae, includes wheat related crops, larger minimum compared other tribes. It appears that occurrence exclusively rather (> 3.4 pg/1Cx) chromosomes (MC ≥ 0.5 pg) restricted Triticeae. origin x = 7 12 lineages was apparently not an increase size, whereas fusion caused size. evolutionary aspects base number are discussed, new numbers presented, first polyploid (2 n 4 20) model plant Brachypodium distachyon s.s.

Язык: Английский

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Harnessing Genomics for Breeding Lantana camara L.: Genotyping and Ploidy Testing of Clonal Lines Through ddRADseq Applications

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(10), С. 4898 - 4898

Опубликована: Май 20, 2025

Lantana camara L. is sold worldwide for ornamental purposes, although it also characterized by high invasiveness potential. Genetic and molecular data available are still poor, breeding performed through conventional methods. This study focused on a genotyping analysis the ddRADseq method an experimental collection of lantana clonal lines to evaluate potential techniques in performing marker-assisted breeding, favour variety registration guaranteeing plant protection species. Although genetic uniformity was observed population, unique profile assigned every line, indicating effectiveness approach used. Interestingly, low degrees heterozygosity were observed. In addition, possibility inferring ploidy levels SNP profiles assessed since would avoid necessity previous biological knowledge use fresh materials. Ploidy interest obtain less invasive triploids. Flow cytometry chromosome counting used inference assessment. An nQuack framework provided correct results majority lines, confirming its effectiveness. These findings encourage adoption systems help breed minor species such as camara.

Язык: Английский

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Karyotype Analysis, Genomic and Fluorescence In Situ Hybridization (GISH and FISH) Reveal the Ploidy and Parental Origin of Chromosomes in Paeonia Itoh Hybrids

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(19), С. 11406 - 11406

Опубликована: Сен. 27, 2022

Itoh hybrids are intersectional in Paeonia L. with sect. Moutan and as paternal maternal parents, respectively. Therefore, these have herbaceous stems improved ornamental value introduced by the parent. Although both of their parents diploids, triploids. Moreover, parental origin chromosomes has not been extensively studied. This study systematically analyzed genome size, ploidy, karyotype compared them taxa. monoploid size was different, it significantly different from that parents. However, varieties two taxa wild species, probably due to rearrangements caused artificial selection. Further analysis, correlation hierarchical clustering could identify hybrids. Verification through genomic fluorescence situ hybridization (GISH FISH) suggested for three sets hybrids, were parent, one One first other a cultivated variety. GISH label peonies Moutan, huge complex genomes species. Meanwhile, 5S rDNA-based FISH applied Paeonia, which may be used ploidy assessment. work give insights into utilization hybrid resources.

Язык: Английский

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Mastering DNA Content Estimation by Flow Cytometry as an Efficient Tool for Plant Breeding and Biodiversity Research

Methods and Protocols, Год журнала: 2023, Номер 6(1), С. 18 - 18

Опубликована: Фев. 12, 2023

Flow cytometry gives a unique opportunity to analyze thousands of individual cells for multiple parameters in course minutes. The most commonly used flow application plant biology is estimation nuclear DNA content. This becomes an indispensable tool different areas research, including breeding, taxonomy, development, evolutionary biology, populational studies and others. content analysis can provide insight into natural ploidy changes that reflect processes, such as interspecific hybridization polyploidization. It also widely processing samples with biotechnologically induced changes, instance, plants produced by doubled haploid technology. Absolute genome size data cytometric serve useful taxon-specific markers since vary between taxa. often allows the distinguishing species within genus or even subspecies. Introducing method lab extremely appealing, but new users face significant challenge learning instrument management, quality sample preparation processing. Not only complex method, have features make demanding research subject. Without proper training, researchers risk damaging expensive publishing poor data, artifacts unreproducible results. We bring together information from our experience, key papers online resources step protocols give starting point exploring abundant literature.

Язык: Английский

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Endoreduplication changes in five in-vitro-grown crops upon treatment with silver nanoparticles

Plant Cell Tissue and Organ Culture (PCTOC), Год журнала: 2023, Номер 155(2), С. 455 - 466

Опубликована: Июль 8, 2023

Abstract Silver nanoparticles (AgNPs) are now often utilized in agriculture and horticulture. One of the common applications is to use AgNPs as antimicrobial agents tissue cultures instead using standard sterilization procedures. However, addition beneficial effects they have also cyto- genotoxic ones, inducing DNA damage changing cell cycle dynamics. The aim this study was determine effect at different concentrations (50–100 ppm) on endoreduplication, content, growth seedlings five crops: rapeseed, white mustard, sugar beet, red clover, alfalfa, cultivated vitro. Flow cytometry used establish genome size synthesis patterns roots, hypocotyls, leaves first-leaf-pair seedlings. AgNP-treatment did not influence germination or size, but increased root length endoreduplication intensity. increases were especially pronounced species/organs with high polysomaty. We suggest that enhanced endopolyploidization a defense mechanism against stress induced by causing mitotic division disruption.

Язык: Английский

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