Patterns of Insect Distribution in Fruit Trees of South Romania and Their Role as Bacterial Vectors DOI Creative Commons

Dana S. Copoiu,

Paris Lavín, Corina Iţcuş

и другие.

Diversity, Год журнала: 2025, Номер 17(4), С. 295 - 295

Опубликована: Апрель 20, 2025

This study is the first investigation of tree–insect–bacteria interactions in southern Romania, documenting distribution 19 insect species across various fruit trees and their insect-associated bacterial diversity. Insect were identified through DNA barcoding, while communities Anthomyia, Botanophila, Drosophila, Scaptomyza insects analyzed via 16S rRNA gene sequencing. diversity varied apple, cherry, plum, peach, quince trees, with most showing tree-specific distribution, except for Drosophila melanogaster, which was found on all tree species. Its presence primarily influenced by development stages rather than temperature changes. comprised 51 genera four phyla, predominantly Pseudomonadota Bacillota, that species, suggesting potential role these flies as vectors. Several pathogenic biomarkers within microbiomes, involvement disease transmission, particularly affecting apple cherry trees. also provides report seven being microbiome characterization dipteran associated regional

Язык: Английский

Culturable bacteria associated with Anastrepha fraterculus sp. 1: in search of nitrogen‐fixing symbionts with biotechnological potential DOI Open Access

Julieta Salgueiro,

Ana Laura Nussenbaum, María Inés Marchesini

и другие.

Insect Science, Год журнала: 2025, Номер unknown

Опубликована: Фев. 24, 2025

Anastrepha fraterculus is a significant fruit fly pest in Argentina and other South American countries. Previous studies showed the key role of gut bacteria protection nutrient assimilation flies, particularly importance biological fixation nitrogen (diazotrophy). The presence diazotrophic A. sp. 1 has been demonstrated through molecular, culture-independent methods. This study aimed to characterize composition diversity culturable males from different origins, explore their metabolic roles, focusing on bacteria. Three male groups were studied: wild-caught (WW), lab-reared wild larvae (WL), lab-colony raised (LL). Gut collected characterized via 16S rRNA gene sequencing, with potential diazotrophs screened using selective media (SIL NFb). Phylogenetic analysis mapped across bacterial collection, while biochemical profiling ARDRA (Amplified rDNA Restriction Analysis) used quickly differentiate PCR testing for nifH gene, associated fixation, was also performed. Bacterial highest WW, followed by WL, lowest LL. In LL Enterobacter most frequent genus, Klebsiella dominated WW. Among 20 SIL+ isolates identified, 10 came 9 One these (Enterobacter sp.) tested as supplement adult diet, without showing beneficial effect pheromone calling behavior. NFb+; two had gene. effective rapid diazotroph discrimination. These findings highlight symbiotic eco-friendly management strategies like sterile insect technique (SIT). By bacteria, protein requirements artificial diets could be reduced, cutting costs improving affordability SIT programs.

Язык: Английский

Процитировано

1
Studying the Interaction between <i>Chromobacterium</i> <i>anophelis</i> and the Entomopathogenic Fungus <i>Metarhizium</i> <i>pingshaense</i> in <i>Aedes</i> <i>aegypti</i>, the Dengue Vector in Burkina Faso DOI Open Access
Etienne Bilgo,

Djibril K. Traoré,

Jacques Edounou Gnambani

и другие.

Advances in Bioscience and Biotechnology, Год журнала: 2025, Номер 16(01), С. 1 - 12

Опубликована: Янв. 1, 2025

Процитировано

0
Ontogenetic Analysis of Chelonus formosanus and Diversity of Its Internal Microbiota DOI Creative Commons
Jingjing Jia,

Qing Feng,

Wenhua Huang

и другие.

Insects, Год журнала: 2025, Номер 16(2), С. 180 - 180

Опубликована: Фев. 8, 2025

Chelonus formosanus is a parasitic wasp capable of parasitizing various Noctuidae pests, including the highly invasive Spodoptera frugiperda, and it demonstrates strong pest control potential. Both egg larval stages primarily occur within host organism, total developmental time from to adult approximately 19.62 days. To investigate microbial communities at different stages, we performed 16S rDNA sequencing (V1–V9 region) using PacBio identified 404 bacterial species belonging 61 classes, 116 orders, 182 families, 308 genera across larval, pupal, female, male stages. Bacterial diversity richness varied with Enterobacter Enterococcus dominating in larvae pupae Pseudomonas emerging as dominant female adults. In contrast, adults predominantly resided Ralstonia Achromobacter. The predicted functions bacteria C. are marked by high abundance metabolic pathways. This study provides comprehensive understanding morphology contributes practical pests. Additionally, our findings preliminarily characterized community laying groundwork for its functional study.

Язык: Английский

Процитировано

0
Parasite-Induced Replacement of Host Microbiota: Impact of Xenos gadagkari Parasitization on the Microbiota of Polistes wattii DOI Creative Commons
Deepak Nain, Anjali Rana, Rhitoban Raychoudhury

и другие.

Microbial Ecology, Год журнала: 2025, Номер 88(1)

Опубликована: Март 27, 2025

Язык: Английский

Процитировано

0
Patterns of Insect Distribution in Fruit Trees of South Romania and Their Role as Bacterial Vectors DOI Creative Commons

Dana S. Copoiu,

Paris Lavín, Corina Iţcuş

и другие.

Diversity, Год журнала: 2025, Номер 17(4), С. 295 - 295

Опубликована: Апрель 20, 2025

This study is the first investigation of tree–insect–bacteria interactions in southern Romania, documenting distribution 19 insect species across various fruit trees and their insect-associated bacterial diversity. Insect were identified through DNA barcoding, while communities Anthomyia, Botanophila, Drosophila, Scaptomyza insects analyzed via 16S rRNA gene sequencing. diversity varied apple, cherry, plum, peach, quince trees, with most showing tree-specific distribution, except for Drosophila melanogaster, which was found on all tree species. Its presence primarily influenced by development stages rather than temperature changes. comprised 51 genera four phyla, predominantly Pseudomonadota Bacillota, that species, suggesting potential role these flies as vectors. Several pathogenic biomarkers within microbiomes, involvement disease transmission, particularly affecting apple cherry trees. also provides report seven being microbiome characterization dipteran associated regional

Язык: Английский

Процитировано

0